25 February 2026, Volume 45 Issue 1
    

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  • LI Xi, LUO Qingchun, LU Yanping, SU Jian, ZHAO Dong, ZHENG Jia
    Abstract ( ) Download PDF ( ) HTML ( )   Knowledge map   Save
    As one of the world's six major distilled spirits, Baijiu is widely appreciated for its unique aroma and complex taste. With societal development, people's comprehensive pursuit of health has laid the foundation for the Baijiu industry to move towards high quality and high comfort. To establish a comprehensive and scientific evaluation system for Baijiu drinking comfort and promote the systematic scientific development of Baijiu research, the research and evaluation methods of drinking comfort of Baijiu, including scale assessments, biomarker detection, animal model studies, and intelligent sensory analysis were summarized based on recent domestic and international related studies. On this basis, the potential factors influencing the Baijiu drinking comfort were outlined, with a focus on the impact of rich trace components in Baijiu and the brewing process. More in-depth interdisciplinary research was needed in Baijiu drinking comfort studies to explore the effects of Baijiu components on physiological responses and consider enhancing the drinking experience through adjustment Baijiu component.
  • CHENG Wei, ZHU Qingyun, PAN tianquan, MA Yi, WEI Chunhui, JIANG Chao, XUE Xijia, WU Zaijie, LI Ruilong, LAN Wei
    China Brewing. 2026, 45(1): 7-12. https://doi.org/10.11882/j.issn.0254-5071.2026.01.002
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    The open brewing environment leads to the low production efficiency and poor batch stability in the brewing of Baijiu. Bacillus, as the main functional bacterial genus in the fermented grains, has a significant impact on the microbial community and flavor characteristics of fermented grains. Taking Bacillus as the fortifying strain or constructing its synthetic microbial community with Bacillus as the core, and then applying in the brewing of Baijiu could be used as an important way to improve the quality of Baijiu. In this paper, the synthetic microbial community and its construction methods were introduced, the functions of tetramethylpyrazine (TTMP)-producting Bacillus and the construction methods of synthetic microbial community were elaborated. Effect of enhanced application of Bacillus and its gene-edited strains on the structure of the original microbial community, metabolic system and flavor during the brewing process of Baijiu were reviewed, with the aim of providing references for Baijiu brewing and the improving its flavor quality.
  • TANG Jiahuan, YUE Song, ZHANG Qing, PAN Qianglin
    China Brewing. 2026, 45(1): 13-20. https://doi.org/10.11882/j.issn.0254-5071.2026.01.003
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    As three typical types of Baijiu, the strong-flavor (Nongxiangxing) Baijiu, sauce-flavor (Jiangxiangxing) Baijiu, and Daqu light-flavor (Qingxiang- xing) Baijiu were all distilled spirits that produced in open-environment using grains as raw materials and Daqu as saccharification and fermentation agent. The open production mode makes the effect of environmental microorganisms on the three typical types of Baijiu non-negligible. With the development of technologies such as amplicon sequencing and metagenomics, the impact of environmental microorganisms on the micro-ecology of Baijiu brewing has gradually been analyzed. The analysis methods of environmental microbial communities, community structure analysis and influencing factors of three typical types of Baijiu were summarized, traceability analysis of Baijiu brewing microorganisms was carried out, and functional characteristics of brewing microorganisms was outlined, aiming to provide reference for analyzing environmental microbial community structure, exploring the effect of environmental microorganisms on Baijiu fermentation process, and shaping an environmental micro-ecology conducive to Baijiu fermentation.
  • LI Xianzhi, MAO Qiongli, NING Zhenzhen, YAN Ling, LIU Yang, SHI Hao, ZHU Yan, HU Yang, LI Qiang, YANG Qiang
    China Brewing. 2026, 45(1): 21-27. https://doi.org/10.11882/j.issn.0254-5071.2026.01.004
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    Sorghum, as a primary raw material for Baijiu brewing, its nutritional components and brewing properties directly affect the yield and quality of Baijiu. To clarify the chemical composition of sorghum and its correlation with Baijiu quality, the chemical composition of sorghum, including starch, protein, amino acids, fats, tannins, polyphenols, inorganic elements, aromatic compounds, and cyanides was mainly reviewed. The detection methods of chemical composition for sorghums were categorized into two major categories: chemical analysis and instrumental analysis method, and the effect of chemical components of sorghum on liquor yield, flavor, quality and safety of Baijiu were deeply discussed. The aim of this study was to provide a technical foundation for the analysis, monitoring, and application of sorghum's chemical components, as well as to offer valuable references for the selection and optimization of raw materials in the Baijiu industry, the selection of accurate and efficient sorghum component detection techniques, and the assurance of Baijiu quality.
  • CHEN Yanxin, HE Zeqi, WEN Linfeng, CAO Yong
    China Brewing. 2026, 45(1): 28-35. https://doi.org/10.11882/j.issn.0254-5071.2026.01.005
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    Chinese Huangjiu is a unique fermented alcoholic drink in China, it is recognized as one of the oldest alcoholic drink in the world and is deeply loved by consumers. The taste of Huangjiu is the primary factor for consumers to perceive its quality, and the taste characteristics are closely related to the functional components in Huangjiu. However, existing research on the taste of Huangjiu had certain limitations and lacks systematic elaboration. Therefore, in this paper, the research status of taste substances in Huangjiu was summarized, the perception, formation and analysis techniques of taste substances in Huangjiu were systematically reviewed, and the influencing factors of taste substances were discussed, aiming to further understand the taste substances and characteristics of Huangjiu, and provide a certain scientific basis for the flavor quality and quality control of Huangjiu.
  • ZHAO Yawen, ZHANG Jiayu, LIU Suyun, YANGZHOU Heying, FAN Chunmeng, WANG Guoming, ZHOU Yuhan, LIN Liangcai
    China Brewing. 2026, 45(1): 36-46. https://doi.org/10.11882/j.issn.0254-5071.2026.01.006
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    In order to select and breed superior yeast and lactic acid bacterium, the fermentation capacity, tolerance and flavor metabolism performance of 6 yeast strains and 11 lactic acid bacteria from medium-low temperature Daqu were evaluated using single-strain solid-state fermentation and simulated Baijiu fermentation systems. The selected superior strains were applied to the brewing of light-flavor (Qingxiangxing) Baijiu, by optimizing its fermentation process, and the effect of functional strains on the brewing flavor and microbial community of light-flavor Baijiu were investigated. The results showed that Saccharomyces cerevisiae Y6 and Pediococcus acidilactici Pea30 were screened and applied in the production of light-flavor Baijiu. Under the conditions of inoculation ratio of Saccharomyces cerevisiae Y6 to P. acidilactici Pea30 1:5 (yeast inoculum 1.0×107 CFU/ml, P. acidilactici inoculum 5.0×107 CFU/ml), loading temperature 26 ℃, and fermentation time 11 d, the contents of ethyl acetate and ethyl lactate in the fermented grains were 621.3 mg/L and 102 mg/L, respectively, which significantly increased by 71.9% and 13.5% compared with the control (P<0.05), respectively. A total of 50 volatile flavor components were detected in the base liquor of light-flavor Baijiu with enhanced functional strains. Among them, the contents of ethyl acetate, ethyl lactate and phenylethanol were 596 mg/L, 118 mg/L and 90 mg/L, respectively, which were increased by 71.9%, 13.5% and 21.0%, respectively compared with the control. The contents of isobutanol and n-propanol were 99 mg/L and 39 mg/L, respectively, which were 14% and 25% lower than those of the control. The results of the co-occurrence network analysis of microbial communities shown that after the application of functional strains, the structure of the microbial community was reorganized, and the stability of the brewing microecology was enhanced.
  • WANG Xi, HE Huan, MA Chunyan, WANG Ye, LUO Wei, GAO Zhanzheng, ZHANG Dan
    China Brewing. 2026, 45(1): 47-55. https://doi.org/10.11882/j.issn.0254-5071.2026.01.007
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    The physicochemical indexes, volatile flavor components and microbial community diversity of different grades of medium-high temperature Daqu (special grade Daqu, excellent grade Daqu and common grade Daqu) were detected by conventional detection methods, GC-MS and high- throughput sequencing technology, respectively, and the correlation among the three was analyzed. The results showed that the physicochemical indexes of different Daqu samples were different. The saccharification power (865.10 U), esterification power (617.61 U), fermentation power (0.27 U) and liquefaction power (1.78 U) of special grade Daqu were the highest, the moisture content (9.01%) of excellent grade Daqu was higher, and the acidity (1.06 mmol/10 g) of common grade Daqu was higher. A total of 6 dominant bacterial genera and 5 dominant fungal genera were annotated from Daqu samples. The microbial community structure of different Daqu samples was different. Based on the linear discriminant analysis (LDA) value>3.5 and P<0.05, a total of 5 different bacterial genera and 3 different fungal genera were screened. A total of 47 volatile flavor compounds were detected from Daqu samples, and there were differences in volatile flavor compounds among different Daqu samples. A total of 13 key differential volatile flavor substances were screened based on variable importance in the projection (VIP) value>1, P<0.05 and odor activity value (OAV)>1. The results of correlation analysis showed that the dominant microbial genera had a certain correlation with physicochemical indexes and volatile flavor components of Daqu.
  • NIE Zixuan, ZHANG Yang, HU Jialiang, WANG Jiaqi, WANG Jianguo, XUE Shuhua, LI Yunkui, YANG Huafeng, JIN Guojie
    China Brewing. 2026, 45(1): 56-64. https://doi.org/10.11882/j.issn.0254-5071.2026.01.008
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    Using the wine made from the main grape varieties cultivated in Yanqi, Heshuo and Bohu production areas as the research object, the differences of volatile aroma components were explored using gas chromatography-mass spectrometry (GC-MS) technology combined with sensory quantitative analysis. The results were analyzed by orthogonal partial least squares discriminant analysis (OPLS-DA), hierarchical cluster analysis (HCA), and principal component analysis (PCA). The results showed that a total of 43 volatile aroma compounds were identified in wine samples made from different grape varieties of different producing areas. Based on odor activity value (OAV), 27 key aroma compounds (OAV>1) were screened. HCA and PCA results indicated that there were significant varieties and regional differences in wine aroma composition. The wines from different production areas and varieties could be effectively distinguished by OPLS-DA. Hexyl acetate, phenethyl alcohol, 1-hexanol, hexanoic acid, and others were identified as important aroma components for distinguishing wines brewed by different grape varieties, while ethyl 9-decenoate, geraniol, and others could be used to differentiate aroma characteristics of the same grape variety from different producing areas. Sensory evaluation results showed that Cabernet Sauvignon and Syrah wines from Yanqi exhibited intense black and red berry aromas, while those from Heshuo displayed more prominent green notes. Marselan wines from Heshuo demonstrated stronger black and red berry aromas.
  • ZHANG Qing, HOU Qiangchuan, LI Qing, ZHANG Min, REN Guojun, WANG Yao, GUO Zhuang
    China Brewing. 2026, 45(1): 65-71. https://doi.org/10.11882/j.issn.0254-5071.2026.01.009
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    Bacterial flora structure of medium-high-temperature Daqu in the Hetao area of Bayannur, Inner Mongolia was analyzed by high-throughput sequencing technology, the comparative analysis of Alpha and Beta diversity was conducted with the bacterial flora of medium-high-temperature Daqu in Yingtan area of Jiangxi, the differential flora was analyzed by the linear discriminant analysis effect size (LEfSe), and the bacterial gene function was annotated. The results showed that the richness and diversity of bacterial flora in Daqu from the Hetao area were extremely significantly lower than those in the Yingtan area (P<0.01). Principal coordinates analysis (PCoA) could effectively distinguish medium-high-temperature Daqu from the two regions. The relative abundance of dominant bacterial genera (relative abundance>1.0%) such as Saccharopolyspora, Staphylococcus and Thermoactinomyces in the medium-high-temperature Daqu in the Hetao area was extremely significantly higher than that in the Yingtan area (P<0.01), while the relative abundance of Bacillus, Aquabacterium and Acinetobacter was extremely significantly lower than that in the Yingtan area (P<0.01). The result of LEfSe showed that the differential flora of medium-high-temperature Daqu from the two regions were Bacillus and Saccharopolyspora (LDA value>4.0, P<0.01). The expression level of metabolism, information storage and processing, as well as cellular process and signal transduction gene function in the medium-high-temperature Daqu in the Hetao area was extremely significantly lower than those in the Yingtan area (P<0.01). In conclusion, there were certain differences in the bacterial groups of medium-high-temperature Daqu between the two regions.
  • CHEN Weiwei, HE Zongfu, JIN Na, LI Yingzeng, HUANG Yonghua, ZHANG Qiuyan, GUO Jiying
    China Brewing. 2026, 45(1): 72-78. https://doi.org/10.11882/j.issn.0254-5071.2026.01.010
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    To explore the diversity of fungal communities and the differences in culturable yeasts from different sources of Rushan, the fungal community diversity of 5 Rushan samples collected from Dali Bai Autonomous Prefecture, Yunnan Province was analyzed by high-throughput sequencing technology, and the yeasts in the Rushan were isolated and identified, combined with traditional microbial culture separation and molecular biology techniques. The results showed that there were significant differences in the richness and diversity of fungal communities among the 5 Rushan samples (P<0.05). The dominant fungal genera (average relative abundance>1%) in the Rushan samples were Candida, Aspergillus, Debaryomyces, Dekkera, Rhodotorula, Clavispora and Kluyveromyces. A total of 23 yeast strains were isolated and identified from 5 Rushan samples and classified into 6 genera and 8 species, including 7 strains of Candida zeylanoides and 6 strains of Torulaspora delbrueckii, 1 strain of Kluyveromyces lactis, 1 strain of Candida parapsilosis, 1 strain of Pichia cactophila, 2 strains of Kluyveromyces marxianus, 2 strains of Rhodotorula mucilaginosa, and 3 strains of Clavispora lusitaniae. In conclusion, there were differences in the diversity of the fungal communities and the composition of culturable yeasts of Rushan from different sources.
  • LAI Haimei, WANG Yali, YANG Menglu, MEI Yuan, HUANG Yuli, ZENG Xueqing, ZHAO Nan
    China Brewing. 2026, 45(1): 79-86. https://doi.org/10.11882/j.issn.0254-5071.2026.01.011
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    To investigate the differences in physicochemical properties and flavor of Suancai in different salting pool depth during salting process, the physicochemical indexes, organic acids and volatile flavor compounds were determined by conventional detection methods, HPLC and GC-MS, the principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were carried out based on volatile flavor compounds, and differential volatile flavor compounds were screened based on the variable importance in projection (VIP) values. The results indicated that as fermentation progressed, the differences in sodium chloride, total acid, lactic acid and citric acid contents between the upper and lower layers of Suancai initially increased and then decreased. After six months of salting, the total acid and lactic acid contents in the upper layer were significantly higher than those in the lower layer (P<0.05), while the sodium chloride content in the lower layer was significantly higher than that in the upper layer (P<0.05). A total of 84 volatile compounds were detected in upper and lower layers of Suancai during salting process, among which alcohols, aldehydes/ketones, and esters were the major volatile components. The results of PCA and OPLS-DA indicated that the differences in volatile compounds between the upper and the lower layers of Suancai gradually decreased as fermentation progressed, with little significant difference after salting for six months. 17 differential volatile flavor compounds (VIP>1) were identified between the upper and the lower layers of Suancai, including 2-butenenitrile, 2-methylbutyl isothiocyanate, acetic acid, and lactic acid. The salting pool depth had a significant impact on the physicochemical properties and volatile flavors of salted Suancai. However, as the fermentation time increased, the differences gradually diminish.
  • ZENG Cheng, HE Yuqing, HU Birong
    China Brewing. 2026, 45(1): 87-95. https://doi.org/10.11882/j.issn.0254-5071.2026.01.012
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    To improve the flavor and quality of Enshi pickle, the superior lactic acid bacterium with strong acid-producing capacity was screened from local Enshi radish pickle and pickling water, and morphological observations and molecular biological identification were conducted. The screened strains were applied in pickle fermentation, and their fermentation performance was investigated. The results showed that a total of 4 superior lactic acid bacterial strains were screened, numbered MP4, MP30, MS12 and MS15, respectively. All of them had strong antioxidant activity and could tolerate 2.0 mmol/L H2O2 , 0.8% bile salts and a relatively low pH (2.5). The strain MP4 was identified as Lacticaseibacillus paracasei, the strain MP30 as Lactiplantibacillus plantarum, the strain MS12 and MS15 as Pediococcus acidilactici. The quality of pickle fermented by the four selected strains was better than that of natural fermentation. The total acid content of pickle fermented by strain MS15 was 5.74 g/kg, the elasticity was 0.53, and the sensory score was 89.92, showing the potential of single-strain fermentation of pickle. A total of 37 volatile flavor components were detected in the fermented pickles of the four experimental groups and the control group, including 9 alcohols, 6 aldehydes, 12 esters, 2 alkenes, 3 ketones, 4 acids and 1 amine. The contents of volatile flavor components in the pickle of the experimental groups ranged from 2.45 to 2.83 g/L, which were much higher than the naturally fermented radish pickle (1.84 g/L).
  • FENG Haosen, CHEN Weiqi, LI Xiangluan, GUO Meijun, FEI Yongtao, CAO Rongbing, ZHAO Wenhong, BAI Weidong
    China Brewing. 2026, 45(1): 96-101. https://doi.org/10.11882/j.issn.0254-5071.2026.01.013
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    In order to investigate the effect of rice protein on the quality of rice-flavor (Mixiangxing) Baijiu, using rice-flavor Baijiu brewed from rice as the control group and rice-flavor Baijiu brewed with rice protein addition as the experimental group, the physicochemical indicators of rice-flavor Baijiu were analyzed during the fermentation process by conventional detection methods. The volatile flavor substances were determined by headspace solid- phase microextraction and gas chromatography-mass spectrometry (HS-SPME-GC-MS) technology, and based on the detection results, orthogonal partial least squares discriminant analysis (OPLS-DA) was conducted. The significant differential volatile flavor compounds were screened by the variable importance in projection (VIP) value and the variance analysis of P value. The results showed that the total acid, total ester, and ethanol content in the rice-flavor Baijiu with rice protein addition generally exhibited an increasing trend during fermentation process. At the end of fermentation, the total acid, total ester, and ethanol content in the fermented mash were 5.96 g/L, 3.52 g/L, and 161.16 g/L, respectively. Compared to the control group, the fermented mash with rice protein addition showed an increase in esters compounds such as 1-pentanol, ethyl 9-hexadecenoate, ethyl tridecanoate, ethyl elaidate, and ethyl heptanoate. The total content of volatile flavor compounds (16.97 mg/kg) was higher than that of the control (13.45 mg/kg). The OPLS- DA model could effectively distinguish the samples from the two groups, and a total of 21 significant differential volatile flavor compounds (VIP>1, P<0.05) were screened. The results showed that the addition of rice protein could improve the flavor of rice-flavor Baijiu.
  • ZHU Chunyang, CHEN Ming, LI Feng, WANG Shuo, CUI Chengzhe, LI Baozhi, ZHENG Yu, WANG Chao, SONG Jia
    China Brewing. 2026, 45(1): 102-109. https://doi.org/10.11882/j.issn.0254-5071.2026.01.014
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    In order to improve the aroma character traits of the spice produced by the compound culture medium of ginseng and raspberry, an aroma- producing yeast of Wickerhamomyces anomalus SMR-WA08 isolated from light aroma-type Daqu was used to ferment a ginseng-raspberry composite medium in this study. The effects of initial pH, fermentation temperature, rotational speed, inoculum, seed age, medium volume, and fermentation time on the relative content of aroma compounds were investigated by single-factor experiments. The aroma-producing conditions of strain SMR-WA08 were optimized using response surface methodology. Non-targeted metabolomics was employed to analyze its aroma components and differential metabolites. The results showed that the optimal aroma-producing conditions were initial pH 5.5, fermentation temperature 26 ℃, rotation speed 110 r/min, inoculum 5%, culture age 30 h, medium volume 100 ml/250 ml, and fermentation time 4 d. Under these optimal conditions, the relative content of aroma compounds reached (184.55±2.84)%. Metabolomics analysis indicated significant metabolic differences between the experimental group (T group) and the blank control group (C group). Based on variable importance in the projection (VIP) value>1 and P<0.05, a total of 798 significant differential metabolites were identified, including 333 upregulated and 465 downregulated compounds. The research results shown that W. anomalus fermentation could effectively regulate the relevant metabolic pathways and metabolites in the ginseng-raspberry substrate, thereby enriching and improving the aroma quality.
  • CHEN Jianling, YUAN Yuexiang, SUN Xianfeng
    China Brewing. 2026, 45(1): 110-116. https://doi.org/10.11882/j.issn.0254-5071.2026.01.015
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    To investigate the way of resource utilization of distillery sludge and the evolution rules of bacterial community structure, an ultra-high temperature aerobic composting pilot experiment for 35 d was conducted using distillery sludge as main raw materials, the physicochemical indicators and bacterial community diversity of compost at different stages were determined, and their correlations were analyzed. The results showed that after composting, the fresh mass of the samples decreased by 50.70%, the total nitrogen and ammonium nitrogen content decreased to 3.93% and 6.71 g/kg, respectively, the nitrate nitrogen, humic acid content, humic acid and fulvic acid ratio, and seed germination index increased to 327.46 mg/kg, 59.24 g/kg, 2.73 and 108.90%, respectively, which indicated the effect of composting reduction and harmless treatment were significant in the pilot scale. During the composting process, the richness of bacterial community decreased, the diversity increased, and the bacterial community structure underwent significant evolution. At the phylum level, the dominant phylum gradually evolved from Proteobacteria to Firmicutes; At the genus level, the dominant bacterial genera gradually evolved from Psychobacter and Acinetobacter to Bacillus and Planifilum. Correlation analysis showed that Bacillus and Sporosarcina were significantly positively correlated with compost temperature (P<0.01), Planifilum and Saccharomonospora were significantly positively correlated with seed germination indexes and humic acid contents (P<0.01), Psychrobacter and Acinetobacter were significantly positively correlated with fulvic acid contents (P<0.01), and significantly negatively correlated with humic acid contents and seed germination indexes (P<0.01).
  • CAO Heyao, LIU Mingming, SHI Yang, ZHAO Minhui, LI Shuangyang, ZHAO Jinsong
    China Brewing. 2026, 45(1): 117-125. https://doi.org/10.11882/j.issn.0254-5071.2026.01.016
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    To improve the interface compatibility between distiller's grains fiber and 4,4'-diphenylmethane diisocyanate (MDI) polymer matrix, as well as the mechanical properties of the composite material, surface activation treatment of distiller's grains was conducted using a sodium hydroxide-hydrogen peroxide system. The activated distiller's grains was then blended with MDI to prepare composite materials, and the effect of activation on the physicochemical properties of distiller's grains and its composites, as well as on the interface compatibility, mechanical properties, and thermal stability of the composites were investigated. The results showed that activation with the sodium hydroxide-hydrogen peroxide system disrupted the structure of lignocellulose in distiller's grains, reduced the silica content on the inner surface by 63.9% and on the outer surface by 62.9%. This effectively enhanced the surface reactivity of distiller's grains fibers, strengthened their interface compatibility with MDI resin, and promoted the formation of good interfacial crosslinking at high temperatures, thereby improving the mechanical properties of the composite material. The optimal preparation process conditions of the distiller's grains-MDI composite were as follows: MDI addition 25%, the hot-pressing temperature 120 ℃, and the hot-pressing process parameters were divided into three stages: 1 MPa for 30 s, 5 MPa for 6 min, and 2 MPa for 30 s. Under these conditions, the composite prepared with activated distiller's grains fibers exhibited a flexural strength of 39.29 MPa and an elastic modulus of 3 929.35 MPa. Compared with the composite materials prepared with inactivated fibers, the flexural strength and elastic modulus were significantly increased by 58.5% and 86.0%, respectively. Furthermore, the activation had no significant effect on the thermal stability of the distiller's grains-MDI composite materials. This study provided an effective approach for the large-scale resource utilization of distiller's grains waste.
  • WANG Zixuan, DUAN Zhirou, LI Wanzeng, HU Xinyuan, YANG Jingwen, WANG Jingwen, YANG Dan, ZHANG Lulu
    China Brewing. 2026, 45(1): 126-133. https://doi.org/10.11882/j.issn.0254-5071.2026.01.017
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    To screen out the monoterpenoids biotransform strain with excellent performance, using α-pinene, β-myrcene and limonene as substrates, primary screening was performed by sole carbon source method, rescreening was performed by headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS). Strains capable of biotransformation monoterpenoids (α-pinene, β-myrcene and limonene) were isolated from tobacco leaves, identified by morphological observation and molecular biology, and the growth characteristics analysis and safety evaluation were carried out. The results indicated that two strains capable of biotransforming monoterpene compounds were isolated and screened from tobacco leaves, numbered as CJ14 and L-2. The main biotransformation products of strain CJ14 were (-)-trans-pinocarveol, dihydrocarveol, and carvone, etc., and the main biotransformation products of strain L-2 were ipsdienol, dihydrocarveol, and limonene, etc. Strains CJ14 and L-2 were identified as Burkholderia sp. and Bacillus sp., respectively. The optimal growth temperature for both strains CJ14 and L-2 was 30 ℃, the optimal rotation speed was 300 r/min and 150 r/min, respectively, and the optimal pH was 9 and 5, respectively. The safety evaluation results indicated that the strains CJ14 and L-2 showed no hemolytic phenomenon, among them, the strain L-2 was sensitive to 11 antibiotics. In summary, strain L-2 had excellent growth performance and safety, and could be used as a strain capable of biotransformation monoterpenoids to increase the added value of monoterpenoids.
  • WU Shukun, ZENG Chaoqun, HUANG Zhiguo
    China Brewing. 2026, 45(1): 134-139. https://doi.org/10.11882/j.issn.0254-5071.2026.01.018
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    Microbial community structure in different parts of strong-flavor (Nongxiangxing) Daqu was analyzed by high-throughput sequencing technology and its physicochemical indicators were measured. The results showed that the main physicochemical indicators such as acid protease activity, saccharification power, esterification power, fermentation power, and mass loss were all in the order of Daqu surface>middle layer of Daqu>inner layer of Daqu. In terms of microbial diversity, the dominant bacteria genera in the Daqu surface were Bacillus, Thermoactinomyces, Pseudomonas, Weissella, Lactobacillus, Cyanobacteria, Leuconostoc and Pantoea, and the dominant fungi genera were Thermoascus, Aspergillus, Candida, Wickerhamomyces, Rhizomucor and Byssochlamys. The dominant bacteria genera in the middle layer of Daqu were Bacillus, Thermoactinomyces, Pseudomonas, Weissella and Lactobacillus, and the dominant fungi genera were Thermoascus, Rhizomucor, Aspergillus, Rhizopus and Thermomyces. The dominant bacteria genera in the inner layer of Daqu were Bacillus, Thermoactinomyces, Pseudomonas, Lactococcus and Apibacter, and the dominant fungi genus was Thermoascus. The overall pattern of dominant microbial community counts were Daqu surface>middle layer of Daqu>inner layer of Daqu. The redundancy analysis (RDA) results indicated that the enzyme activity indexes and fermentation capacity indexes were strongly related to Aspergillus, Byssochlamys, Candida, Wickerhamomyces, Bacillus, Weissella, Lactobacillus, Cyanobacteria, Leuconostoc, Acetobacter, and the acidity was strongly related to Rhizomucor, Rhizopus and Thermomyces.
  • DU Shaoji, WANG Yurong, DU Xinyong, WANG Chaoqing, HOU Qiangchuan, GUO Zhuang
    China Brewing. 2026, 45(1): 140-147. https://doi.org/10.11882/j.issn.0254-5071.2026.01.019
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    To investigate the quality differences between medium-high- and high-temperature Daqu in the Northwest Canal region of Shandong province, the fungal community structure, and moisture indicators (water content, water activity, bound water, immobile water, and free water content) were analyzed by PacBio SMRT sequencing technique, conventional physicochemical testing methods, and low field-nuclear magnetic resonance (LF-NMR) technology. Additionally, the correlation between dominant fungal species and moisture indicators was analyzed. Sequencing results revealed that Aspergillus proliferans was the dominant species in medium-high-temperature Daqu, with an average relative abundance of 55.08%, whereas Thermomucor lanuginosus predominated in high-temperature Daqu, with an average relative abundance of 34.66%. A total of 12 significantly differential substances were identified by linear discriminant analysis effect size (LEfSe), which showed distinct differences in the two types of Daqu samples. Among them, A. proliferans and T. lanuginosus could serve as biomarkers for medium-high-temperature Daqu and high-temperature Daqu, respectively. Moisture indicators analysis results demonstrated that medium-high-temperature Daqu had significantly higher water content (11.00%), water activity (0.641), immobile water (5.04%), and free water (0.96%) (P<0.05), whereas high-temperature Daqu exhibited significantly higher bound water content (94.96%) (P<0.05). Correlation analysis results showed that there was a significant or extremely significant correlation between the dominant fungal species and the moisture indicators (P<0.05 or P<0.01). These findings provided data support for elucidating the quality variations among different types of Daqu in Northwest Canal region of Shandong province.
  • WANG Yifei, GAO Tingting, WANG Shuo, HOU Jiayi, GAO Bingning, YANG Chenyu, SONG Jia
    China Brewing. 2026, 45(1): 148-157. https://doi.org/10.11882/j.issn.0254-5071.2026.01.020
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    In order to enhance the wine flavor and overcome weak environmental adaptability and low ester productivity of ester producing yeasts. The yeast strains were isolated from light-flavor (Qingxiangxing) Daqu and then high-yield ester strains screened, the ethanol resistance and high temperature resistance characteristics were measured, and identified by molecular biology. The culture conditions of the strain were optimized by single factor experiments and response surface methodology, and the volatile flavor components of the wine fermented by the screened strain and commercial strain preparation agent (RW) were determined. Results showed that a strain 021233 with high biomass under ethanol 12%(V/V) and temperature 40 ℃ was screened, and the strain 021233 was identified as Saccharomycopsis fibuligera by molecular biology techniques. The optimal culture medium for the strain 021233 were as follows: glucose 4.6%, yeast extract 1.2% and MgSO4 0.1%. Under these conditions, the bacterial biomass reached its maximum value (OD600 nm value=7.88) after culture at 30 ℃, 200 r/min for 24 h. 28 esters and 14 alcohols were detected in the wine samples fermented by the strain 021233, all of which were higher than those in the wine samples fermented with agent RW. Compared with RW, the relative content of esters in the wine samples fermented by the strain 021233 increased by 262.6%, significantly improving the flavor quality of the wine. The strain 021233 was expected to play an important role in enhancing the flavor of wine.
  • LIU Meijuan, JIANG Lei, ZHOU Junlan, HE Yuehui, WANG Shujun
    China Brewing. 2026, 45(1): 158-165. https://doi.org/10.11882/j.issn.0254-5071.2026.01.021
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    The pullulanase gene was cloned from Thermotoga neapolitana KCCM 41025 by molecular biology technology. The recombinant pullulanase was expressed through cold shock induction in Escherichia coli BL21 and its enzymatic properties were analyzed. The enzyme was applied to the preparation of porous lotus seed starch, with structural characterization performed using scanning electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction and the characteristics of lotus seeds porous starch were studied. The results demonstrated that the optimal reaction temperature for the enzyme was 70 ℃, and the optimal pH was 6.0. The microstructure of porous lotus seed starch hydrolyzed by recombinant pullulanase Pul-K25 for 12 h was the optimal. The porous lotus seed starch treated with pullulanase Pul-K25 did not undergo any changes in functional groups and the crystal type unchanged, while the crystallinity increased by 15% after enzymatic hydrolysis for 12 h. The porous lotus seeds starch was prepared by enzymatic hydrolysis combined with ultrasound, which increased its water absorption rate from 64.28% to 123.03%, oil absorption rate from 80.40% to 85.33%, solubility from 1.93% to 4.01%, and swelling capacity from 2.08% to 3.68%. The results showed that the recombinant pullulanase had the application prospect of preparing porous lotus seeds starch.
  • LEI Gao, QUAN Shujing, ZHANG Yongzhan, LIU Ling, LI Shanshan, MU Qi, LIU Dehai, ZHAO Junkun, YANG Wenling
    China Brewing. 2026, 45(1): 166-171. https://doi.org/10.11882/j.issn.0254-5071.2026.01.022
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    The basic physicochemical indicators of the rhizsphere soil samples of kiwifruit from Kuiwen Village (KW), Quyuan Gang Village (QYG), and Wangying Village (WY) in Xixia County, Nanyang City were detected by conventional detection methods. The microbial community diversity was analyzed by Illumina MiSeq high-throughput sequencing technology, and the correlation was analyzed. The results showed that there were significant differences in pH, available phosphorus content, available potassium content and alkali-hydrolyzable nitrogen content among the three samples (P<0.05). There was no significant difference in the bacterial community diversity and species abundance among the three samples (P>0.05). The fungal community diversity of the QYG sample was the highest, while that of the KW sample was the lowest. However, there was no significant difference in the species abundance of the fungal community among the three samples (P>0.05). A total of 51 bacterial phyla and 16 fungal phyla were annotated from the 3 samples, including 16 dominant bacterial phyla and 6 dominant fungal phyla. Among them, Bacillota and Actinomycetota, as potential pathogenic control bacteria for kiwifruit canker, were all dominant phylum in the 3 samples. The physicochemical properties of the soil had a significant impact on the distribution of microbial communities in the rhizosphere of kiwifruit. Among them, the Bacillota was extremely significantly negatively correlated with the content of available potassium (P<0.01), and the Actinomycetota was significantly positively correlated with the content of available potassium (P<0.05), and was significantly negatively correlated with pH (P<0.05). pH was highly extremely significantly positively correlated with the Rozellomycota (P<0.001). The research results laid a foundation for the screening and identification of bacterium that could prevent kiwifruit canker disease.
  • LAN Chaohua, ZHOU Wei, ZHOU Xiaoli, GUO Zhengxiang, PENG Tao, WANG Lingxiang, CHENG Xinkai, LIU Yinan, JIANG Renjie, YE Beiling, HE Chaojiu
    China Brewing. 2026, 45(1): 172-177. https://doi.org/10.11882/j.issn.0254-5071.2026.01.023
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    Using strong-flavor (Nongxiangxing) Daqu as the research subject, the yeast in Daqu was isolated and purified by traditional culture and separation methods. Based on fermentation performance tests, tolerance experiments, and flavor metabolism capacity analysis, high-tolerance aroma- producing yeast strains were screened, and the strains were identified using molecular biology techniques. The results showed that two high-tolerance aroma-producing yeasts (numbered as JM-1 and JM-2) were isolated from the strong-flavor Daqu. Both strains could tolerate pH 2, ethanol volume fraction 7%, and temperature 40 ℃, and had the ability to produce ethyl caproate and ethyl acetate through metabolism, respectively. The strains JM-1 and JM-2 were identified as Nakaseomyces glabratus and Clavispora lusitaniae, respectively. These findings suggested that both yeast strains had potential as functional microorganisms for application in Baijiu production.
  • A Ruhan, LU Bingyun, HE Mengchao, ZHONG Heng, HAN Xinglin, LUO Ruiqi, WANG Deliang
    China Brewing. 2026, 45(1): 178-185. https://doi.org/10.11882/j.issn.0254-5071.2026.01.024
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    A toxicological evaluation for edible safety of Amaranthus caudatus seeds was conducted. In the acute oral toxicity test, SPF mice was administered a one-time gavage at a dose of 10 000 mg/kg. For the oral toxicity study, SD rats of half male and half female were divided into three dose groups (1 600 mg/(kg·d), 4 000 mg/(kg·d), and 10 000 mg/(kg·d)) and a blank control group, and were continuously fed for 28 d. The erythrocyte micronucleus test was performed using SPF mice, wherein the number of polychromatic erythrocytes containing micronucleus was observed after gavage, the micronucleus occurrence rate was calculated, and the ratio of polychromatic erythrocytes to total erythrocytes was determined. The acute toxicity test results showed that median lethal dose (LD50)>10 000 mg/kg, which was classified as practically non-toxic according to acute toxicity grading standards. The 28 d oral toxicity test results indicated that no statistically significant differences (P>0.05) were observed in body mass gain, hematological parameters, blood biochemical indexes, organ coefficients between any of the dose groups and the control group. In the mouse erythrocyte micronucleus test, no statistically significant differences in the micronucleus incidence were observed between the dose groups and the control group, and no abnormal changes were noted in the ratio of polychromatic erythrocytes to total erythrocytes [polychromatic erythrocyte/(polychromatic erythrocyte+normochromatic erythrocyte)]. Therefore, A. caudatus seeds have not shown any toxic effects and were considered as a safe food raw material. This study provided a toxicological basis for their further development and widespread application in the food industry.
  • PU Fujuan, XUE Jie, FAN Zhejia, YU Jiajun, SHI Jun, XV Na, ZHU Jiawei, WANG Meiqi, WU Yun
    China Brewing. 2026, 45(1): 186-195. https://doi.org/10.11882/j.issn.0254-5071.2026.01.025
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    To clarify the effect of vine age on the quality of 'Marselan' grapes and their corresponding dry red wines from the Yanqi region of Xinjiang, using conventional detection methods, high-performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-QqQ-MS), gas chroma- tography-mass spectrometry (GC-MS), and sensory evaluation, the physicochemical indicators, anthocyanin composition, volatile flavor compounds, and sensory quality of grapes from vines aged 2, 8, 9, 11, and 15 years and their corresponding dry red wine samples were analyzed. The results indicated that no significant differences in physicochemical parameters among grape fruits of different vine ages (P>0.05), but there were obvious differences in the physicochemical indicators of the dry red wine produced. The contents of monomer anthocyanins including delphinidins, methyl-delphinidins and methylanthocyanins were the highest in the grape fruits from 9-year-old vine (10.63 mg/kg, 16.32 mg/kg and 35.77 mg/kg respectively). The total content of monomer anthocyanins and volatile flavor substances in its dry red wine samples was the highest (451.2 mg/L and 327 mg/L respectively). A total of 45 volatile flavor substances were detected in the wine samples with grapes from 9-year-old vine, among which 11 were unique and 17 were key volatile flavor substances (OAV≥1). The wine samples produced from 9-year-old vine grapes had the highest sensory score (82.0 points), and exhibiting a deep purple-red color with brilliant luster, transparent body, and a rich, elegant bouquet displaying typical wine aromas. In summary, dry red wine produced from 9-year-old vine grapes demonstrated superior quality.
  • HUANG Yuanhang, ZHANG Qiuyue, XIONG Zhi, SHEN Xin, LUO Taimei, HAN Long
    China Brewing. 2026, 45(1): 196-203. https://doi.org/10.11882/j.issn.0254-5071.2026.01.026
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    In order to screen superior non-Saccharomyces yeast suitable for the fermentation of Chaenomeles sinensis fruit wine, yeast was isolated from the epidermis of high-acid fruits and natural fermentation broth. The strains were screened through Durham tube fermentation, strain tolerance and fermentation performance determination tests. The screened strains were identified through molecular biology techniques. The volatile flavor components of the C. sinensis fermented by the screened strain were analyzed by electronic nose technology combining with principal component analysis (PCA). The results showed that among the 144 isolated strains, 4 strains could simultaneously tolerate glucose mass concentration of 500 g/L, pH 2, SO2 mass concentration 250 mg/L, and ethanol volume fraction of 9%. Strains G26, G28, G29 and N1 possessed excellent ester and ethanol production capabilities as well as low H2S production capacity. After identification, strain N1 was Meyerozyma guilliermonclii, strain G26 was Metshnikowia pulcherrima, strain G28 was Wickerhamomyces anomalus, G29 was Lachancea waltii. The results of the electronic nose showed that strains N1 and G26 exhibited the most significant changes in alcohol-aldehyde compounds and nitrogen oxides in fermented C. sinensis juice samples. The PCA results based on the analysis response values of the electronic nose indicated that the fermented juice by strain N1 shown the most significant difference from the other samples. The screened and identified excellent non-Saccharomyces yeasts were suitable for the fermentation of C. sinensis, and provided strain resources and theoretical basis for the brewing of C. sinensis fruit wine.
  • YANG Tingfei, WANG Fengqing, SONG Hongning, GUI Yuan, MA Yanfang, ZHANG Suyi, LIU Jun, LI Li, LI Junxun
    China Brewing. 2026, 45(1): 204-209. https://doi.org/10.11882/j.issn.0254-5071.2026.01.027
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    To promote the resource utilization of distiller's grains, Baijiu distillers' grains were fermented by compound microbial agents in solid-state. Using contents of acidic detergent fiber (ADF) and neutral detergent fiber (NDF) as evaluation indicators, the compound ratio of the 3 strains for Bacillus subtilis X-1, Lactobacillus buchneri, Aspergillus oryzae were optimized. The fermentation process conditions of distiller's grains were optimized through single-factor tests and orthogonal tests, and the quality indicators were analyzed. The results indicated that the optimal strain compard ratio were L. brucellosisB. subtilis X-1∶A. oryzae = 2∶1∶1 (V/V), and the optimal fermentation conditions for distiller's grains were: fermentation temperature 37 ℃, inoculum 10%, initial moisture 58%, and fermentation time 48 h. Under this optimized condition, the contents of ADF and NDF in the fermented distiller's grains were 19.94% and 23.13%, respectively, and the reduction rates of ADF and NDF were 31.18% and 45.04%, respectively. The contents of crude protein (CP), acid-soluble protein (ASP), and ASP/CP were 16.03 g/100 g, 4.14 g/100 g, and 25.83%, respectively, which increased by 30.12%, 41.40%, and 8.67%, respectively, compared with those before fermentation. The total acid content and pH were 13.14 g/kg and 5.06, respectively. Aflatoxin B1, vomitoxin and zearalenone toxin were not detected before or after fermentation, and their safety met the relevant standard requirements. In conclusion, the solid-state fermentation of distiller's grains with compound bacterial agents could effectively degrade the fiber components of distiller's grains and enhance their nutritional value.
  • LU Zhangyu, ZHANG Yujing, LIU Qingshan, BAI Wenbin, GAO Zhenfeng, XU Nv
    China Brewing. 2026, 45(1): 210-215. https://doi.org/10.11882/j.issn.0254-5071.2026.01.028
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    The physicochemical indexes of Jinza 51# (japonica sorghum) and Jinnuo 3# (waxy sorghum) were investigated, and then were used to brew Shanxi aged vinegar, respectively. The physicochemical indexes and flavor compounds were detected by conventional detection methods, high-performance liquid chromatography (HPLC), and headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME- GC-MS) during the brewing process, and the effects of different sorghum varieties on the brewing quality of Shanxi aged vinegar were investigated. The results showed that there were certain differences in the physicochemical indexes between the two sorghum varieties. Compared with Jinza 51#, the Jinnuo 3# exhibited significantly higher contents of moisture and amylopectin (P<0.05), while displaying significantly lower contents of fat, tannin and amylose (P<0.05). Jinnuo 3# demonstrated superior fermentation characteristics, with significantly higher contents of total acid (6.60 g/100 ml), non-volatile acid (2.08 g/100 ml), total ester (3.04 g/100 ml), and tetramethylpyrazine (36.27 mg/l) in the newly leached vinegar (P<0.05). In contrast, the contents of reducing sugar (1.70 g/100 ml) and total flavonoids (86.75 mg/100 ml) were significantly lower (P<0.05). The total organic acid content in newly leached vinegar brewed with Jinnuo 3# (4.26 g/100 ml) was 20.99% higher than that in newly leached vinegar brewed with Jinza 51#, with notable increases in acetic acid, lactic acid and succinic acid. The volatile flavor compounds in newly leached vinegar brewed with Jinnuo 3# were predominantly esters, whereas alcohols and phenols were the major volatile components in newly leached vinegar brewed with Jinza 51#.
  • ZHANG Yunfeng, WU Junrui, MU Delun, TIAN Ye, TIAN Chao, FAN Zhaochen, DOU Shihe, WU Rina
    China Brewing. 2026, 45(1): 216-222. https://doi.org/10.11882/j.issn.0254-5071.2026.01.029
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    In order to study the effect of mixed-strain fermentation of different species of Lactiplantibacillus plantarum on the quality of Chinese sauerkraut, the Chinese sauerkraut was prepared using cabbage as the raw material, and compounded with different ratios of L. plantarum ww and Fs 5-5 as the fermenting agents. With single-bacterium fermentation as the control group, the physicochemical indexes, textural characteristics, volatile flavor substances and sensory qualities of Chinese sauerkraut during the fermentation process were analyzed. The results showed that the Chinese sauerkraut samples could be matured by fermentation for 7 d at 30 ℃. All quality indexes of Chinese sauerkraut fermented with mixed-bacteria were better than those of single-bacterium fermentation. The quality of Chinese sauerkraut prepared by L. plantarum ww and Fs 5-5 in the ratio of 1∶2 was optimal, and at the end of fermentation, its pH, nitrite, reducing sugar contents and hardness were 3.13, 0.67 mg/kg, 1.56 g/L and 24.9 N, respectively. The color parameters L* value, a* value and b* value were 48.70, -0.04 and 9.96, respectively. The sauerkraut was bright yellow in color, crunchy and moderate hardness, with a sensory score of 85.8. A total of 42 volatile flavor substances in all Chinese sauerkraut samples were detected, including 10 acids, 6 aldehydes, 6 alcohols, 8 esters, 5 phenols, 5 ketones, and 2 ethers, with a content of 48.14 μg/ml. Mixed-bacteria fermentation not only could improve the quality of Chinese sauerkraut and enhance the taste and aroma compared to single-bacterium fermentation, but also provide a new experimental method for rapid fermentation of Chinese sauerkraut.
  • LIU Meng, HONG Jinming, LV Wenhao, FAN Fengjiao, ZHANG Jianming
    China Brewing. 2026, 45(1): 223-229. https://doi.org/10.11882/j.issn.0254-5071.2026.01.030
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    In order to analyze the effect of heat treatment on the proliferation of osteoblasts promoted by walnut protein and enzymatic hydrolysate, walnut protein and enzymatic hydrolysate were treated by different heat treatment methods (65 ℃/30 min, 72 ℃/10 s, 85 ℃/10 min, 95 ℃/10 min), and using the untreated group as control. After co-culturing walnut protein and enzymatic hydrolysis products with osteoblasts, the morphology of osteoblasts was observed, and the osteoblasts were stained with hematoxylin eosin (HE) staining and alkaline phosphatase (ALP) staining to observe their activity. The proliferation rate of osteoblasts promoted by walnut protein and enzymatic hydrolysate was determined by MTT method, and the effect of walnut protein and enzymatic hydrolysate on the proliferation rate of osteoblasts at 24 h, 48 h and 72 h were analyzed. The results showed that for walnut protein, the proliferation rate of osteoblasts treated with 65 ℃/30 min was the highest at 24 h (67.51%). For enzymatic hydrolysates, the proliferation rate of osteoblast with 65 ℃/30 min treatment was the highest at 24 h (39.39%), but the overall proliferation rate was slightly lower than that of walnut protein. At the same time, with the increase of heat treatment temperature, the proliferation rates of walnut protein and enzymatic hydrolysate showed a downward trend, and both were lower than the control group. In summary, the heat treatment method of 65 ℃/30 min on walnut protein and enzymatic hydrolysate had the optimal effect on promoting osteoblast proliferation. The results provided data support for the subsequent development of bone-promoting functional products and their sterilization.
  • FANG Qiang, FENG Zuoshan, BAI Yujia, SHI Qingheng, CUI Yuxin, ZHAO Yunfei, XU Xiangrong
    China Brewing. 2026, 45(1): 230-238. https://doi.org/10.11882/j.issn.0254-5071.2026.01.031
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    Using Xinjiang Jun-jujube as raw material, the jujube brandy was produced by low-temperature distillation (≤70 ℃) technology. The effect of different aging methods (natural aging, UV-accelerated aging, and microwave-accelerated aging) on the aroma quality of jujube brandy were investigated. The physicochemical indexes, color parameters, volatile aroma components, and sensory evaluation of jujube brandy under different aging methods were analyzed using conventional analytical methods, headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography- mass spectrometry (GC-MS), and the principal component analysis (PCA) was performed. The results showed that compared with the total ester (2.98 g/L), total phenol (1 144 mg/L), and tannin (0.77 g/L) of naturally aged jujube brandy for 12 months, the total esters (2.76 g/L, 2.83 g/L), total phenols (1 039 mg/L, 1 102 mg/L), and tannins (0.73 g/L, 0.74 g/L) of jujube brandy aged by UV-accelerated and microwave-accelerated all slightly decreased. The UV-accelerated aging jujube brandy had the highest volatile acid content (2.35 g/L) and alcohol content (44.16%vol). A total of 160, 159 and 158 volatile aroma substances were respectively detected in jujube brandy aged naturally for 12 months, UV-accelerated aging and microwave-accelerated aging. A total of 51 key aroma substances (relative odor activity value (ROAV)>1) were screened. The sensory evaluation results showed that UV-accelerated and microwave-accelerated aging treatment could accelerate the formation of sensory characteristics such as floral, fruity and woody aromas of jujube brandy. In conclusion, UV-accelerated and microwave-accelerated aging treatments could shorten the aging period and enhance the aroma quality, but natural aging still had advantages in terms of the complexity and harmony of aroma.
  • SU Yufan, LI Yan, Alatengtogusi, XIA Yanan
    China Brewing. 2026, 45(1): 239-247. https://doi.org/10.11882/j.issn.0254-5071.2026.01.032
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    Beef jerky was prepared by using the hind leg meat of cattle and mixing (1∶1) fermentation with Lactobacillus delbrueckii YN-01 and Pediococcus acidilactici S3-3. The effect of fermentation conditions such as inoculum, fermentation temperature and time on the texture, flavor, color, sensory and physicochemical quality indicators of fermented beef jerky were explored by conventional detection methods, texture analyzer, colorimeter and electronic nose, etc. The results showed that fermentation starter inoculum, fermentation temperature and time all had significant effect on the sensory, physicochemical, texture and color quality indicators of fermented beef jerky (P<0.05). Under the conditions of inoculum 5%, fermentation temperature 33 ℃ and time 24 h, the pH value, sensory score, moisture content, water activity, L* value, a* value, b* value, elasticity, adhesiveness, resilience, hardness, cohesion and chewiness of fermented beef jerky were 6.37, 92.70, 42.43%, 0.844, 26.13, 2.8, 6.50, 0.79, 8 630.13, 0.257, 14 195.57 g, 0.578, 6 824.41, respectively. E-nose detection results of fermented beef jerky flavor showed that different inoculum, fermentation temperature, and fermentation time significantly affected the composition of odor substances in the product (P<0.05). The dominant odorants were organic sulfides (W2W), terpenes (W1W), alkanes (W1S), ethanol (W2S), alkanes (W3S), and nitrogen oxides (W5S). Principal component analysis (PCA) could effectively distinguish fermented beef jerky samples with different fermentation conditions based on electronic nose response values.
  • YIN Ruiyang, GAO Zhiyuan, LIU Xia, GUO Liyun, SONG Yumei, ZHAO Dongrui
    China Brewing. 2026, 45(1): 248-255. https://doi.org/10.11882/j.issn.0254-5071.2026.01.033
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    To explore the influence laws of changes in malt amylase activity and reducing sugar content under different malting processes on the formation of malt flavor substances, the amylase activity and reducing sugar content of wheat grains at different malting stages under different malting processes were determined. The contents of key malt aroma characteristic substances were determined by gas chromatography-mass spectrometry (GC-MS) technology, and the correlation analysis was conducted. The results showed that reducing the pH (6.5) of the wheat soaking water, adding enzyme activators (gibberellin and MgCl2) during the germination process, increasing the degree of wheat soaking (45%) and lowering the roasting temperature (83 ℃) could all increase the amylase activity of wheat grains during the withering process, promote the generation of fructose, glucose and maltose during the germination process and the consumption of fructose and glucose during the roasting process, and enhance the generation of key malt aroma characteristic substances. Among them, the effect of adding gibberellin was the optimal. The total amylase activity of the wheat grains after wilting was (1 019.26±87.02) U/g, the glucose production in the germination stage was (3.21±0.66) g/100 g, and the glucose consumption in the roasting stage was (1.79±0.30) g/100 g. The production of six key malt aroma characteristic substances was (4 542.57±201.33) μg/100 g. Correlation analysis indicated that the glucose consumption during the malting was positively correlated with the generation of key malt aroma characteristic substances, and the activity of malt amylase after wilting was positively correlated with the generation of glucose and key malt aroma characteristic substances.
  • HE Xiaoyu, LI Quancen, WANG Pengyi, LI Na, LIU Bin, ZENG Feng
    China Brewing. 2026, 45(1): 256-262. https://doi.org/10.11882/j.issn.0254-5071.2026.01.034
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    Taking the residue of Lentinula edodes after polyphenol extraction as the raw material, polysaccharides were extracted by ultrasonic-assisted water extraction method. Using the lentinan yield as the evaluation index, the extraction process of lentinan was optimized by single-factor experiments combined with response surface methodology and the radical scavenging ability of lentinan was investigated. An in vivo antioxidant test model was constructed using Caenorhabditis elegans to investigate the effect of lentinan on oxidative stress-related indicators in C. elegans. The results showed that the optimal extraction conditions for L. edodes polysaccharides were as follows: ultrasonic power 240 W, solid-to-liquid ratio 1∶30 (g∶ml), ultrasonic time 50 min, and temperature 75 ℃. Under these optimal conditions, the yield of lentinan was 1.89%. In vitro antioxidant experiments results demonstrated that lentinan exhibited strong scavenging abilities against 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) and hydroxyl radicals, with half-scavenging concentrations (IC50 value) of 1.91 mg/ml, 0.58 mg/ml, and 0.16 mg/ml, respectively. In vivo antioxidant experimental results showed that lentinan significantly enhanced the activities of superoxide dismutase (SOD) and catalase (CAT) (P<0.05), and significantly inhibited the accumulation of malondialdehyde (MDA) (P<0.05) in the body of C. elegans. Therefore, lentinan had significant antioxidant activity.
  • HUANG Xiaojing, SHEN Tian, NIU Ruimin, XU Zehua, CHEN Weiping, GUO Ping
    China Brewing. 2026, 45(1): 263-268. https://doi.org/10.11882/j.issn.0254-5071.2026.01.035
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    To improve the quality of 'Merlot' grape quality in the eastern foothills of Helan Mountain, using the 'Merlot' grape with three training systems (vertical cordon, 'V'-shaped, "Chang" shaped) as experimental materials, different intensification leaf removal treatments (removing 2, 4, and 6 leaves at the base of the fruiting branches) were carried out at mid-veraison, using the corresponding treatment without leaf removal as control. The CIELAB color space parameters, phenolic substances, anthocyanin composition and content of the fruit were determined. A comprehensive evaluation was performed using principal component analysis. The results indicated that leaf removal could increase reducing sugar content, reduce total acid content, pH and single grain weight, and improve grape maturity, promoting the accumulation of phenolic substances in grape fruits. At harvest, the total phenol content under the "Chang" shaped training system with 2 leaves removed (T1) and the vertical cordon with 2 leaves removed (Z1) was significantly increased by 22.47% and 31.25%, respectively. Under the 'V' shaped training system with 6 leaves removed (V3), the contents of 3'-malvidin and delphinidin were 57.41% and 48.24% higher than those in the control. Leaf removal could increase the red and green tone (a*), yellow and blue tone (b*) of grape fruit by "Chang" shaped training system, while the removal of 4 to 6 leaves significantly reduced the hue (h*ab) value of grape fruit under different training system. The comprehensive principal component evaluation results showed that for the 'V'-shaped training system, removing 6 leaves at mid-veraison most significantly enhanced grape fruit quality. For the vertical cordon and "Chang" shaped systems, the removal of 2 leaves at mid-veraison was the optimal. Therefore, different canopy management method should be selected based on the climate of different years.
  • ZHANG Lei, WANG Shuqing, HE Yihao, CHENG Kaiyuan
    China Brewing. 2026, 45(1): 269-275. https://doi.org/10.11882/j.issn.0254-5071.2026.01.036
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    To accurately predict the temperature of fermented grains and identify the area of gas emission from fermented grains, thereby guiding the steaming bucket loading robot to perform even bed-laying, using the temperature data of steaming bucket loading fermented grains at Fenglin Brewery as the research object, a precise prediction model for the temperature of fermented grains was constructed by integrating infrared thermal imaging technology with multi-layer extended long short-term memory network (xLSTM), replacing the traditional fully connected layer with Kolmogorov- Arnold Network (KAN) layer, and optimizing the model parameters using the improved marine predators algorithm (IMPA), and the prediction performance was evaluated. The results showed that the temperature prediction performance of the IMPA-xLSTM-KAN model was superior to that of the traditional Long Short-Term Memory Network (LSTM), Marine Predators Algorithm (MPA)-xLSTM-KAN, and IMPA-xLSTM. The mean absolute error (MAE), mean square error (MSE), root mean square error (RMSE), and determination coefficient (R2) were 0.182, 0.053, 0.237, and 0.934, respectively. Moreover, the deployment test of the model on the Rockchip RK3588 embedded platform showed that the single inference time was only 7.7 ms, which met the requirements of real-time control. The effectiveness of the IMPA-xLSTM-KAN model provided a theoretical basis for the precise steam detection by steaming bucket loading robot, and was of great significance for improving the technical level of Baijiu brewing.
  • WANG Lei, LU Qinghua, WU Hanlin, TAO Yunian, WANG Zhanrong, MA Xinying, QIN Haopeng, LI Yali
    China Brewing. 2026, 45(1): 276-281. https://doi.org/10.11882/j.issn.0254-5071.2026.01.037
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    In this study, naked oat craft beer was prepared using barley malt and naked oat as the main raw materials. The fermentation conditions were optimized by single factor tests and response surface tests, and the quality indexes were analyzed. The results showed that the optimal fermentation conditions were as follows: naked oat addition 24%, hop addition 0.3 g/L, yeast addition 6%, and main fermentation temperature 19 ℃. Under these optimal conditions, the sensory score of the naked oat craft beer was 91.23 points, the original wort concentration, fermentation degree, alcohol content, density, chorma, pH and foam persistence were 8.39 °P, 79.23%, 2.82%vol, 1.005 6 g/cm3, 11 EBC, 3.77, and 150 s, respectively. The contents of total acid, diacetyl, carbon dioxide were 2.13 ml/100 ml, 0.1 mg/L, 0.42%, respectively. All the physicochemical indexes complied with the relevant national standards. The naked oat craft beer contained 14 kinds of amino acids, including 5 essential amino acids, 3 conditionally essential amino acids and 6 non-essential amino acids, and its amino acid contents were higher than that of commercially available beers.
  • FENG Xueying, NI Hui, HOU Qiangchuan, YE Mingbo, GUO Zhuang, WANG Yurong
    China Brewing. 2026, 45(1): 282-287. https://doi.org/10.11882/j.issn.0254-5071.2026.01.038
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    Using four commercially available sauce-flavor (Jiangxiangxing) Baijiu produced in Maotai town and four from other production regions as the research objects, the differences of volatile flavor components of commercially available sauce-flavor Baijiu produced from different production areas were compared and analyzed based on gas chromatography ion mobility spectroscopy (GC-IMS) technology and multivariate statistical analysis method. The results showed that a total of 51 volatile flavor compounds were identified in the eight Baijiu samples, including 24 esters, 9 alcohols, 10 aldehydes, and 8 ketones. Principal component analysis (PCA) indicated that there were differences in volatile flavor components between Maotai town and other production areas of sauce-flavor Baijiu. Based on the relative odor activity value (ROAV), a total of 6 key volatile flavor compounds (ROAV>1) were screened out. Among them, ethyl isopentate, ethyl octanoate and isopentyl acetate contributed more significantly to the aroma of Maotai town's sauce-flavor Baijiu, with ROAV values of 82.25, 5.56 and 1.52, respectively. Ethyl hexanoate, ethyl valerate and ethyl butyrate contributed more significantly to the aroma of other production regions' sauce-flavor Baijiu, with ROAV values of 33.19, 32.55 and 10.48, respectively.
  • LI Lixia, CHENG Zhou, LI Dan, ZHONG Xiaoxue, LUO Xueping
    China Brewing. 2026, 45(1): 288-294. https://doi.org/10.11882/j.issn.0254-5071.2026.01.039
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    A post-column derivatization liquid chromatography method was developed for the simultaneous determination of fructose, glucose and sucrose in tea wine. Asahipak NH2P-50 4E chromatographic column (250 mm×4.6 mm, 5 μm) was used, the isocratic elution was carried out with mobile phase consisted of acetonitrile-water (75∶25, V/V) containing 0.5% H3PO4, the flow rate was 0.8 ml/min and column temperature was 40 ℃. Post- column derivatization involved oxidation with periodic acid (2.0 g/L) and color development using phenol reagent (1.0 g/L) at 120 ℃, followed by detection at a wavelength of 670 nm. The results showed that fructose had a good linear relationship (R2>0.999) within the mass concentration range of 25.0-500.0 mg/L, and glucose and sucrose had a good linear relationship within the mass concentration range of 50.0-1 000.0 mg/L. The detection limit were 2.58-9.64 mg/L, and the quantification limit were 7.82-29.21 mg/L. The recovery rates of standard addition ranged from 96.55% to 98.73%, and the relative standard deviations of the precision and stability tests results were both less than 2.00%. The contents of three kinds of carbohydrates in 11 types of commercially available tea wines of 4 categories were detected by this method. The results showed that the glucose was detected in all the tea wines, with contents ranging from 0.038 to 111.36 g/L, and fructose was detected in 10 types of tea wines, with contents ranging from 0.023 to 115.90 g/L. Among them, the content of glucose and fructose in blended tea wine was the highest. Sucrose was only detected in 3 types of Lujiu-type tea wines, with contents ranging from 0.012 to 0.380 g/L. This method was simple to operate, had low reagent costs and high recovery rates, and could provide new detection technical support for the process research, product development and standard setting of tea wine.
  • ZHANG Xiaosu, LI Zhaoyun, HE Fei, JIANG Hongjiu, CHEN Anze, SHE Lulu, TANG Pinghua
    China Brewing. 2026, 45(1): 295-299. https://doi.org/10.11882/j.issn.0254-5071.2026.01.040
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    To achieve rapid discrimination of sauce-flavor (Jiangxiangxing) Baijiu from different process, the spectral data of three sauce-flavor Baijiu with different processes (Daqu process, crushed-sorghum process, and flavor-blended process) after alkaline heating treatment was collected using ultraviolet spectroscopy. Combining the machine learning algorithms of Support Vector Machine (SVM), Random Forest (RF) and K-Nearest Neighbor (KNN), classification and discrimination models of full wavelengths (220-600 nm) and dual wavelengths (277 nm, 327 nm) were established respectively. The performance of the classification models was evaluated by precision rate, recall rate and F1 score, and the classification models were verified. The results showed that after alkaline heating treatment, the three types of sauce-flavor Baijiu exhibited absorption peaks at wavelengths of 277 nm and 327 nm, but there were significant differences in absorbance values within the full wavelength range (220-600 nm). Ranked by absorbance value from high to low in order were Daqu process, crushed-sorghum process, and flavor-blended process. The performance evaluation results of the model showed that the SVM model constructed based on the full wavelength and dual wavelength ranges achieved 100% accuracy, recall rate and F1 score for the detection of three types of process Baijiu samples. The prediction results of the verification samples showed that the dual-wavelength SVM classification model had the optimal recognition effect. The recognition accuracy rates of Daqu process and flavor-blended process samples were 100%, and that of crushed-sorghum process samples were 90%, indicating that the classification model could distinguish sauce-flavor Baijiu with different process.
  • TIAN Luqin, WU Lan, JIANG Lili, ZHU Xiaochun, SUN Youlan, SHANG Yuhao, YOU Xiaolong, HU Jianfeng, WANG Diqiang
    China Brewing. 2026, 45(1): 300-305. https://doi.org/10.11882/j.issn.0254-5071.2026.01.041
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    In order to establish a discriminative analysis method for authenticity of sauce-flavor (Jiangxiangxing) Baijiu based on chemometrics, the volatile flavor compounds in true (T) and fake (F) sauce-flavor Baijiu were analyzed by gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS), the discriminative model for true and fake Baijiu was established by principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), and the discriminative results were verified. The significant differential volatile flavor compounds (VIP>1, P<0.05) that distinguish true and fake Baijiu were screened out based on the variable importance projection (VIP) value and the P value of variance analysis, and the prediction model was established. The results showed that 100 and 92 volatile flavor compounds were detected in true or fake Baijiu samples, respectively. The contents of esters, alcohols, and acids were relatively higher in both true or fake Baijiu samples (4 951.35 mg/L and 3 098.40 mg/L; 3 465.81 mg/L and 1 054.62 mg/L; 2 385.68 mg/L and 1 958.08 mg/L, respectively). The classification accuracy of the PCA and OPLS-DA models reached 100%. A total of 58 significant differential volatile flavor compounds were screened out to establish a predictive model. The correlation coefficient R2 of the prediction set was 0.998, the root mean square error of prediction (RMSEP), the root mean square error of cross-validation (RMSECV), and the estimated root mean square error (RMSEE) of the training set were 0.023, 0.064, and 0.049, respectively, indicating the discriminant model was stable and reliable, and could effectively distinguish between true and fake sauce-flavor Baijiu.
  • LIN Shao, DONG Yuxuan, YANG Hecai, HU Jinyi
    China Brewing. 2026, 45(1): 306-311. https://doi.org/10.11882/j.issn.0254-5071.2026.01.042
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    Wine supply chain competitiveness is the core driving force for promoting industrial transformation and upgrading and achieving high-quality development. Based on the theory of competitive advantage, the analytical framework from three dimensions: cost advantage, resource and capability, and differentiation advantage was constructed, the paths to enhance the competitiveness of the wine supply chain were revealed from a multi- configuration perspective by using the fuzzy set qualitative comparative analysis (fsQCA) method. The results showed that a single factor didn't constitute a necessary condition for high supply chain competitiveness, but the synergy of multiple factors significantly affected the competitiveness level. There were three improvement paths, namely "cost-marketing driven type", "comprehensive advantage type", and "resource-brand dominant type", and there was a substitution relationship among different paths. By identifying the key configurations that affect the competitiveness of the wine supply chain, it not only expanded the theoretical boundary of supply chain competitiveness research, but also provided a basis for differentiated path selection for wineries with different resource endowments, which had important practical value for promoting the high-quality development of the wine industry.
  • LIU Yang, WANG Jinyun, YUE Qian, XIE Shengli
    China Brewing. 2026, 45(1): 312-320. https://doi.org/10.11882/j.issn.0254-5071.2026.01.043
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    As the core link of wine industry, wine grape cultivation was faced with the problems of data dispersion, disorder, large amount of data and insufficient utilization, and lack of professional talents. Knowledge graph could effectively organize and manage knowledge and perform knowledge calculation as an emerging knowledge representation tool. The construction of wine grape cultivation knowledge graph was of great significance to improve the quality of wine. The knowledge of wine grape cultivation was sorted out using the seven-step method, and the ontology model of wine grape cultivation was constructed. The idea and method of constructing knowledge graph of wine grape cultivation based on large language model were proposed to realize knowledge extraction, knowledge fusion, knowledge processing, knowledge storage and knowledge application. Based on the knowledge graph, the knowledge in the field of wine grape cultivation was deeply excavated, aiming to provide rich and diverse knowledge services for the entities engaged in grape cultivation, promote the development of wine grape cultivation in the direction of specialization, personalization, precision and intelligence, promote the digital transformation and upgrading of the wine industry, and enhance the new quality productivity level of the industry.
  • MIAO Li, CHEN Zhihao, ZHANG Bei, YANG Wenguang
    China Brewing. 2026, 45(1): 321-327. https://doi.org/10.11882/j.issn.0254-5071.2026.01.044
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    Industrial clusters effectively enhance industrial competitiveness through mechanisms such as resource sharing, knowledge spillover and technological innovation. However, while gaining competitive advantages, industrial clusters also face risks that threaten the high-quality development of industrial clusters. In this paper, using the wine industry in the Yanqi Basin as research objects, the risk factors within the wine industry cluster were identified, and their interrelationships were determined through the first-hand descriptive text analysis data obtained from in-depth interviews and expert opinions. Based on the direct relationships among the risk factors, an adjacency matrix was established, followed by hierarchical stratification of these factors to construct an Interpretative Structural Modelling (ISM) for the cluster risk system. The results showed that the risk factors for wine industry cluster in Yanqi Basin formed a five-level hierarchy, with varying degrees of induction and scopes of influence on cluster risk system of wine industry cluster. All underlying elements in the risk factor system structure model had a direct or indirect impact on the top-level factor of technological research and development capital investment. Natural resource endowment and internal management inside enterprises were important internal factors contributing to the risk of wine industry cluster, while industrial promotion policies, inter-firm collaboration models, and industrial regulation regulations constituted significant external factors. This paper proposed targeted countermeasure to address risks at different hierarchical levels, aiming to prevent internal and external risks brought about by the wine industry cluster and promote high-quality development of wine industry effectively.
  • YANG Yubo, NIU Junjie, WANG Houyin, CAO Nian, ZHONG Kui, XIONG Xiaolin, YANG Fan, YUN Zhenyu, XU Yang, TU Huabin, ZHAO Lei, ZHANG Yao, QIN Zhen, XIANG Ping, SHI Bolin
    China Brewing. 2026, 45(1): 328-333. https://doi.org/10.11882/j.issn.0254-5071.2026.01.045
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    The sauce-flavor (Jiangxiangxing) Baijiu has rich flavor attributes, extremely high sensory experience and tasting value. Its sensory description was formed at the Fifth National Alcoholic Drinks Tasting Conference and is mainly used by professional liquor tasters. However, the perception level and tasting skills of the professional tasters are usually higher than those of consumers, and the way of tasting liquor is quite different from the daily drinking of consumers. There are significant differences between describing the perceptual characteristics of Baijiu from an analytical perspective and conveying the experience and feelings of drinking from a consumption-oriented perspective. In this paper, through a Python web crawler program, comments on the sauce-flavor Baijiu were captured from online platforms. The sensory descriptive words in the online comments were classified by means of text preprocessing and text mining. The results showed that a total of 6 435 comments on sauce-flavor Baijiu were captured and classified into sensory descriptive words and perceptual descriptive words. The descriptive words of the perception type exhibited the characteristics of "viewing the whole, being relatively broad, preferring contrast, and emphasizing experience". The reduction in the number and scope of the descriptive words of the perception type indicated the differences in the attention, perception and expression of the sensory quality of the sauce-flavor Baijiu between consumers and tasters. The result verified the rationality of constructing description words for the consumption experience of sauce-flavor Baijiu and the feasibility of analyzing the focus of consumers' perception based on online comments.