25 May 2026, Volume 45 Issue 5
    

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  • XIE Yingping, PAN Qiuhong
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    ‘Orange’ wine is produced from white grapes using a process similar to red wine’s “skin-contact fermentation”, belonging to the category of skin-contact white wine. This technique breaks through the traditional white wine model which requires clarification and juice extraction prior to fermentation, resulting in a wine body that combines the structural complexity of red wine with the refreshing characteristics of white wine. In recent years, it has garnered significant attention in both the international market and academic fields. This review systematically summarizes recent advances in key process parameters, sensory and flavor chemical characteristics, and main influencing factors in ‘orange’ wine production, with a particular focus on elucidating the regulatory mechanisms of maceration duration on phenolic and aroma compound profiles. Based on this, the review proposed key directions for future process optimization and chemical analysis, aiming to provide a scientific basis for the precise development and sustainable industrial development of this wine variety.
  • WU Yuquan, YIN Yanting, DU Juan, WANG Song, WANG Guixin, FAN Hongchen
    China Brewing. 2026, 45(5): 7-15. https://doi.org/10.11882/j.issn.0254-5071.2026.05.002
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    Wheat beer haze is a key factor affecting beer appearance, sensory quality, and stability, and it is also regarded as one of the core quality attributes in wheat beer research and production. In wheat beer, proteins and polyphenols can form insoluble complexes through hydrogen bonding, hydrophobic interactions, and other intermolecular forces, which are considered the main sources of haze formation. In addition, different metabolic activities of yeast during the fermentation and storage may further influence the stability of beer haze. This article focuses on wheat beer haze and systematically reviews the influencing factors of haze, analytical and detection techniques, as well as control measures. Future research directions are also discussed. The aim is to provide a reference for elucidating the molecular mechanisms and formation patterns of wheat beer haze, and to offer a scientific basis for quality improvement and process optimization.
  • GUO Jiaqi, ZHANG Yongqing, ZHANG Liqiang, WANG Songtao, SUN Xiaotao, TU Rongkun, LIU Xiaogang, DONG Wei
    China Brewing. 2026, 45(5): 16-22. https://doi.org/10.11882/j.issn.0254-5071.2026.05.003
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    The Baijiu industry constitutes a vital segment of China’s food industry. As a significant by-product generated during the Baijiu brewing process, distillers’ grains offer substantial potential for resource-efficient and high-value valorization, which has garnered considerable attention within the industry. However, the processing of distillers’ grains faces several challenges and technical bottlenecks, including high moisture content, susceptibility to degradation, and a low cellulose conversion rate. Consequently, developing a variety of resource utilization technologies for distillers’ grains and establishing a “circular industrial chain” model remain critical challenges that must be addressed to advance the sustainable development of the Baijiu industry. This paper presents a review of the research and utilization status of distillers’ grains over the past five years, systematically analyzes the existing barriers to their resource utilization, and proposes potential future development directions, with the aim of providing theoretical support for promoting the healthy and environmentally sustainable development of the Baijiu industry.
  • YANG Yuanping, CHEN Mingxue
    China Brewing. 2026, 45(5): 23-30. https://doi.org/10.11882/j.issn.0254-5071.2026.05.004
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    As a typical representative of China’s traditional solid-state fermented distilled spirits, Baijiu’s distinctive flavor and exceptional quality depend on the synergistic interactions of complex microbial communities. Recently, with the rapid advancement of multi-omics technologies, the functional potential, metabolic mechanisms, and interaction networks of microorganisms involved in Baijiu brewing have been comprehensively elucidated. In this review, the diversity characteristics of microbial communities during Baijiu brewing processes were systematically summarized, with particular emphasis placed on the mechanistic roles of core functional microbial groups in flavor compound synthesis. The metabolic pathways, functional genes, and interaction networks of microbial communities were deeply analyzed using multi-omics approaches, through which systematic regulatory mechanisms from gene expression to metabolite synthesis were revealed. In response to biosafety risks associated with open fermentation processes in Baijiu production, including exogenous contamination and the accumulation of hazardous metabolites, risk control strategies were comprehensively reviewed, ranging from raw material source control and multi-parameter monitoring during fermentation to synthetic assembly of functional microbial consortia. This review aims to provide a theoretical basis for achieving precise microbial regulation and enhancing quality and safety in Baijiu production.
  • SUN Li, LI Shu, CHEN Fei, YU Hui, HUANG Shenghong, LIAO Yanjing, WANG Yuting, NIU Mansi, WEN Yue, WANG Jiali, WANG Songtao, LI Delin, LUO Zhiyong, Xiao Chen
    China Brewing. 2026, 45(5): 31-39. https://doi.org/10.11882/j.issn.0254-5071.2026.05.005
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    As a traditional low-alcohol fermented food, Laozao (fermented glutinous rice) faced limitations in market competitiveness due to the singularity of commercial strains and insufficient flavor complexity. To address this, six aroma-producing yeast strains were isolated from sweet Jiuqu and Baijiu fermentation environments and applied in co-fermentation to enhance the volatile flavor profile of the product. These strains were molecularly identified as Saccharomycopsis fibuligera, Kazachstania exigua, Cyberlindnera fabianii, Pichia kudriavzevii. Through sensory evaluation, alcohol production capacity tests, and analysis of volatile compounds, superior strains were selected for co-fermentation experiments in Laozao. The results indicated that the introduction of aroma-producing yeasts significantly increased the variety and content of alcohols and esters, particularly key flavor components such as phenethyl alcohol, isoamyl alcohol, and ethyl caproate. The T1-3 group (C. fabianii). achieved the highest sensory evaluation score (88), while the T1-4 group (S. fibuligera) exhibited the highest total volatile compound content, reaching (51.17 ± 2.84) μg/g. Additionally, co-fermentation improved the utilization efficiency of reducing sugars. Orthogonal partial least squares-discriminant analysis (OPLS-DA) further revealed differences in aroma composition among the yeast fermentation groups and identified five key differential flavor compounds. The study demonstrated the positive role of aroma-producing yeasts in improving the flavor of Laozao, providing a scientific basis for enhancing the quality of traditional fermented foods and developing microbial resources.
  • CHENG Shuang, GAO Fang, LIU Bingbing, LI Xuesi, LI Wenjun, GUO Shuxian
    China Brewing. 2026, 45(5): 40-47. https://doi.org/10.11882/j.issn.0254-5071.2026.05.006
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    To explore the yeast diversity and ester-aroma producing characteristics in the fermented grains of Songhe strong-flavor (Nongxiangxing) Baijiu. Yeasts were firstly isolated and purified via traditional culture methods, followed by morphological observation and molecular biology identification. Furthermore, ester-aroma producing yeasts were screened and their ester-aroma producing properties were investigated. The results showed that a total of 29 yeast strains were isolated from the fermented grains, belonging to 4 genera and 5 species, including 4 strains of Naumovozyma castellii, 9 strains of Pichia fermentans, 1 strain of Pichia manshurica, 3 strains of Zygosaccharomyces rouxxii, and 13 strains of Saccharomyces cerevisiae, with S. cerevisiae as the dominant species. Three superior ester-aroma producing strains were screened out, namely Z. rouxxii CL-19, S. cerevisiae CL-21 and S. cerevisiae CL-24. Among them, CL-21 exhibited the best resistance; strain CL-19 had a strong ester-aroma producing capacity, producing 11 kinds of esters with a relative content of 45.64%.
  • LONG Liling, LEI Xuejun, ZHENG Jia, ZHAO Dong, LUO Huibo
    China Brewing. 2026, 45(5): 48-55. https://doi.org/10.11882/j.issn.0254-5071.2026.05.007
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    High esterase-producing strains were screened from Daqu with high esterification power and identified by morphological observation combined with molecular biological techniques. The strain with the highest esterase production was subjected to tolerance analysis, and its effects on the four major esters during Huangshui fermentation were determined. The results showed that 67 mold strains, 66 bacterial strains and 7 yeast strains were isolated from Daqu, and 6 high esterase-producing strains were screened. Among them, Paecilomyces nostocoides DM100 and Aspergillus tubingensis ZM266 exhibited the highest esterification power, reaching 81.90 and 81.76 mg/(g·7 d), respectively. A. tubingensis ZM266 exhibited stronger tolerance and higher ester production capacity in the esterification of Huangshui than P. nostocoides DM100. It could grow under the stress conditions of pH 3, 8% (V/V) ethanol and 42 ℃, and significantly increased the contents of the four major esters (ethyl caproate, ethyl acetate, ethyl lactate, ethyl butyrate) during the esterification of Huangshui.
  • SHAN Qimuge, LI Li, QIN Liqin, KANG Xinyue, ZHOU Xin, LIANG Shuying, TANG Ping, LI Changwen, CHEN Peng, LU Anmou
    China Brewing. 2026, 45(5): 56-64. https://doi.org/10.11882/j.issn.0254-5071.2026.05.008
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    Taking the fermented grains from stacking and pit fermentation of different rounds of sauce-flavor (Jiangxiangxing) Baijiu as research materials, physicochemical indices, functional microorganisms and volatile flavor compounds of fermented grains were analyzed using conventional detection methods, real-time fluorescence quantitative polymerase chain reaction, and headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry, and their correlations were further analyzed. The results showed that the moisture showed a fluctuating trend, the acidity and ethanol content increased gradually, while the reducing sugar decreased progressively, with significant differences among different fermentation rounds. The abundance of functional microorganisms presented a decreasing trend as fermentation proceeded, and varied greatly among rounds. Pichia kudriavzevii and Lactobacillaceae were identified as the absolutely dominant microorganisms in the fermented grains, with higher relative abundance in the initial two rounds (Xiasha and Zaosha) than in other rounds. Zygosaccharomyces bailii and Schizosaccharomyces pombe maintained relatively high relative abundances from the 1st to the 5th fermentation round. Saccharomyces cerevisiae exhibited the lowest relative abundance. Esters, alcohols, and acids were the main volatile flavor compounds in the fermented grains, which increased continuously with the advancement of fermentation, while the other substances exhibited relatively minor fluctuations. As the fermentation rounds progressed, the phenols were gradually reduced, whereas the other flavor components (excluding ketones) elevated gradually. Partial least squares discriminant analysis (PLS-DA) results revealed obvious differences in the species and number of flavor compounds in fermented grains among different rounds. Correlation analysis indicated that P. kudriavzevii, Lactobacillaceaeeae and S. cerevisiae were positively correlated with each other, and certain correlations existed between the functional microorganisms and volatile flavor compounds.
  • SHEN Kaiwei, WANG Xueli, ZHANG Xuyi, MU Bo, QIU Shuyi
    China Brewing. 2026, 45(5): 65-73. https://doi.org/10.11882/j.issn.0254-5071.2026.05.009
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    To address the issue of insufficient flavor complexity in pitaya wine fermented with pure Saccharomyces cerevisiae, non-Saccharomyces were isolated and screened from naturally fermented pitaya wine in this study. Strains characterized by prominent aroma production and weak alcoholic pungency were selected via sensory olfactory evaluation, followed by identification through morphological observation and molecular biological techniques. Their stress tolerance properties were further assessed, and mixed-strain fermentation combining the screened strain with S. cerevisiae SY was performed to brew pitaya wine. The mixed-culture fermentation parameters were optimized using single-factor tests coupled with orthogonal experiments, and volatile flavor compounds in pitaya wines produced by single-strain and mixed-strain fermentation were systematically analyzed. The results revealed that an aroma-enhancing yeast strain, designated DBS-1, was isolated and identified as Pichia manshurica. This strain displayed favorable growth performance under the conditions of pH 3.0, 8% ethanol concentration and 18 °Brix initial sugar content. The optimal fermentation conditions for pitaya wine were determined as follows: initial sugar content of 22 °Brix, initial pH of 4.5, inoculum of 4%, and a mixing ratio of SY to DBS-1 of 1:1. Under these optimized conditions, the resulting pitaya wine attained an alcohol content of 8.5% and a high sensory evaluation score of 95.5. A total of 45 volatile flavor compounds were identified in the mixed-fermentation pitaya wine, among which 20 compounds were classified as key volatile components (odor activity value (OAV) ≥ 1). Compared with pure S. cerevisiae fermentation, mixed-strain fermentation increased the total contents of esters, acids and alcohols by 82.4%, 47.6% and 37.7%, respectively. Furthermore, it elevated the OAVs of key aroma compounds including ethyl hexanoate, octanoic acid and phenylethanol, thereby effectively improving the overall flavor balance and sensory quality of pitaya wine.
  • CHEN Liping, HU Xueling, LIU Mengtong, PENG Yongcong, SHI Zhangyan, ZHANG Lin, ZHONG Qiuping
    China Brewing. 2026, 45(5): 74-83. https://doi.org/10.11882/j.issn.0254-5071.2026.05.010
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    To elucidate the effect of fermentation temperature on the composition of amino acid and derivative metabolites in lychee wine, the differences in amino acids and their derivatives in lychee wine fermented at 10, 20, and 30 ℃ were investigate with liquid chromatography-mass spectrometry combined with multivariate statistical analysis. The regulatory mechanism of temperature on the metabolic pathways of amino acids and their derivatives was revealed by Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway enrichment analysis. The results showed that a total of 92 amino acids and their derivatives were identified, and 80 differential metabolites were screened based on the variable importance in projection (VIP) value > 1 and P < 0.05. The relative contents of valine, γ-aminobutyric acid, and other substances were higher in samples fermented at 30 ℃; the relative contents of leucine, phenylalanine, and others were the highest in samples fermented at 20 ℃; while 10 ℃ was not conducive to the retention and metabolism of amino acids. Multivariate statistical analysis revealed that the composition characteristics of the top 10 up-regulated differential metabolites (meeting the screening criteria and ranked by P-value) varied significantly at different temperatures: fermentation at 10 ℃ was mainly enriched with amoxicillin, N-acetyl-L-tyrosine, etc.; the 20 and 30 ℃ groups shared common features, dominated by 2-aminoisobutyric acid, 3-aminobutyric acid, 2-aminobutyric acid, etc. Compared with the unfermented group, a series of statistically significant (P < 0.05) positive and negative correlations existed among the up-regulated amino acids and their derivatives at different temperatures. The differential metabolite pathways were mainly arginine and proline metabolism, with the highest differential abundance score observed at 20 ℃. In conclusion, fermentation of lychee wine at 20 ℃ was more favorable for the utilization and biotransformation of amino acids.
  • ZENG Juan, ZHOU Wen, XU Yi, DENG Peng, WANG Jingxia, ZHANG Cai, ZHANG Chongjun, XIONG Dingzhao, ZHU Kang
    China Brewing. 2026, 45(5): 84-92. https://doi.org/10.11882/j.issn.0254-5071.2026.05.011
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    High-throughput sequencing, non-metric multidimensional scaling (NMDS) and linear discriminant analysis effect size (LEfSe) were used to analyze the microbial community structure in high-temperature Daqu with floral-fruity compared with ordinary high-temperature Daqu. Headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS) was used to detect the volatile flavor substances in two types of high-temperature Daqu, and Spearman correlation analyses was applied to explore correlation between dominant microbial genera and volatile flavor substances. The results showed the diversity and structure of bacterial and fungal community structures in high-temperature Daqu with floral-fruity were different from those in ordinary high-temperature Daqu, and the species diversity and richness were significantly lower (P < 0.05). Based on linear discriminant analysis (LDA) values > 4 and P < 0.05, a total of 6 different bacterial genera and 5 different fungal genera were detected from the two types of high-temperature Daqu, among which Kroppenstedtia, Virgibacillus and Thermomyces were the main differential genera in high-temperature Daqu with floral-fruity. The total content of volatile flavor substances (376.14 μg/g) and the content of main flavor substances pyrazines (164.83 μg/g), aromatics (64.83 μg/g) and ketones (61.29 μg/g) in high-temperature Daqu with floral-fruity were higher than those in ordinary high-temperature Daqu. The main volatile flavor substances were positively correlated with Kroppenstedtia, Virgibacillus and Thermomyces. The results of this study revealed the correlation between microbial community structure and flavor formation of high-temperature Daqu with floral-fruity, which can provide a theoretical basis for the quality control of high-temperature Daqu.
  • CHEN Fengxia, YUE Youhan, XU Wensheng
    China Brewing. 2026, 45(5): 93-102. https://doi.org/10.11882/j.issn.0254-5071.2026.05.012
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    To investigate the effects of different Jiuqu (CZQ, FTQ, PDQ) on microbial community structure and the formation of volatile flavor compounds during the fermentation of Jiangxi rice wine, the composition of volatile flavor compounds was analyzed with headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry, the microbial community structure was characterized with high-throughput sequencing technology, and the correlations between them were explored by multivariate statistical methods. The results showed that a total of 62 volatile flavor compounds were detected, and 10 key differential flavor components were identified via principal component analysis (PCA). Among these, benzaldehyde and aromadendrene were found at higher levels in the PDQ group, 2,3-butanediol was more abundant in the CZQ group, and ethyl linoleate predominated in the FTQ group. Microbial diversity analysis revealed statistically significant differences in microbial communities among different starters groups. Among the 10 core bacterial genera, Lactococcus was dominant in the CZQ and FTQ groups, while Lactiplantibacillus and Leuconostoc were predominant in the PDQ group. Among the 8 core fungal genera, Saccharomyces was dominant in the CZQ group, Saccharomyces and Saccharomycopsis were dominant in the PDQ group, and Clavispora was the absolute dominant genus in the FTQ group. Correlation analysis revealed positive correlations between microorganisms and flavor compounds. Specifically, Lactococcus and ethyl palmitate, Gluconobacter and 3-methyl-1-butanol, as well as Mucor and 2-phenylethanol (P < 0.01). In addition, the formation of short-chain fatty acids such as valeric acid and isovaleric acid was also positively correlated with multiple microorganisms (P < 0.01). The results of this study provide a theoretical basis for the further optimization of traditional rice wine fermentation processes and quality standardization.
  • HUO Hongyan, ZENG Yaoying, YU Zhuojun, XIONG Yubo, ZHAO Ye, SHE Jiehai, ZHANG Kai, ZHOU Wenhua
    China Brewing. 2026, 45(5): 103-110. https://doi.org/10.11882/j.issn.0254-5071.2026.05.013
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    The mustard in the natural fermentation process (1-45 days) of dry-salting method under different salt levels (9%, 12%, 15%, 18%, based on mustard mass) was used as the research object. The basic physical and chemical indexes, color and bacterial flora diversity were detected by conventional detection methods and high-throughput sequencing technology. The results showed that the pH value of pickled mustard in each group decreased first and then tended to be gentle, the change of total acid content was opposite, while the contents of reducing sugar, nitrite and amino acid nitrogen increased first and then decreased during the fermentation process. With the increase of salt addition level, the pH value and reducing sugar content of pickled mustard increased, and the total acid content decreased. At 45 days of fermentation, the amino acid nitrogen content of pickled mustard with salt addition level 15% was the highest, and the nitrite content of pickled mustard with salt addition level 12% was the lowest. The color of pickled mustard in each group became darker during fermentation, and the saturation of yellow increased, but the color became brighter with the increase of salt addition level. At 45 days of fermentation, the bacterial flora diversity of pickled mustard with salt addition levels 9% and 12% was higher, and the bacterial flora richness of pickled mustard with salt addition level 15% was the highest. The difference in bacterial flora of pickled mustard in each group was mainly reflected in the relative abundance. At 45 days of fermentation, the main dominant bacterial genera in pickled mustard with salt addition level 9% were Lactiplantibacillus and Corynebacterium, while the main dominant bacterial genera in pickled mustard with other salt concentrations were Staphylococcus. In summary, the suitable salt addition level for pickled mustard should be 12%-15%.
  • KANG Xi, ZHANG Zhengjie, DENG Bo, JIA Junjie, MA Long, ZHAO Xingrong, LUO Rongtao, CHEN Zhilin, GAO Feng, HUANG Zhiguo
    China Brewing. 2026, 45(5): 111-119. https://doi.org/10.11882/j.issn.0254-5071.2026.05.014
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    To investigate the effects of mechanical versus manual brewing methods on the third-round stacked fermented grains of sauce-flavor Baijiu, the microbial community structure and organic acid profiles of the fermented grains before and after stacking fermentation in both mechanical and manual brewing processes were compared by high-throughput sequencing and high-performance liquid chromatography. Additionally, correlation analyses were conducted on the major microbial genera and organic acids. The results showed that the acidity of the manually brewed fermented grains was significantly lower before and after stacking fermentation compared to the mechanically fermented grains (P < 0.01); the dominant bacterial genera before and after stacking fermentation were both Kroppenstedtia and Virgibacillus; before stacking fermentation, the dominant fungal genera were Thermomyces; after stacking fermentation, the dominant fungal genera in the manually and mechanically fermented grains were Pichia and Zygosaccharomyces, respectively; a total of 9 organic acids were identified in the fermented grains samples, with relatively high levels of lactic acid, acetic acid, propionic acid, succinic acid, and formic acid. Acid-producing bacteria such as Acetobacter were enriched in mechanically fermented grains, while microbial communities such as Saccharomyces were enriched in manually fermented grains. Correlation analysis indicates that prior to stacking fermentation, Lactobacillus, Thermoascus, and Thermomyces were the primary acid-producing genera, whereas after stacking fermentation, genera such as Kroppenstedtia became the primary acid-producing genera.
  • LI Shuai, WANG Ying, WU Haihong, LI Dajing, WANG Yu, MA Yanhong
    China Brewing. 2026, 45(5): 120-126. https://doi.org/10.11882/j.issn.0254-5071.2026.05.015
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    To screen for indigenous Saccharomyces cerevisiae strains with superior aroma-producing capability, using the fermentation mash of Xinjiang apricot fruit as the isolation source, strain was screened preliminarily using a 96-well plate high-throughput colorimetric method with p-nitrophenyl-β-D-galactopyranoside as the substrate, and the obtained strains were screened secondarily via simulated apricot wine fermentation experiments. The fermentation characteristics, β-glucosidase and esterase production capacities, and multiple stress tolerances of the target strains were comprehensively analyzed, and morphological observation combined with molecular biological techniques was performed for strain identification. The results showed that 16 yeast strains were preliminarily screened from the fermentation mash of 4 Xinjiang apricot varieties, among which 2 strains with strong aroma-producing performance, designated M1-7-2 and MS-9, were further obtained and identified as S. cerevisiae var. boulardii and S. cerevisiae, respectively. The β-glucosidase activity and esterase activity of strain M1-7-2 were 16.31 and 18.88 U/L, respectively, while those of strain MS-9 were 15.96 and 18.26 U/L, respectively. Both strains exhibited tolerance to stresses of 10-40 ℃, pH 3-7, glucose 300 g/L, SO2 150 mg/L, and 16% ethanol. The results of this study can provide reliable strain resources for the development of apricot wine starters and the quality improvement of apricot wine.
  • DUAN Pengtao, TU Rongkun, JIANG Xiaofeng, LI Xianfang, TU Feiyong, LIU Guangqian, ZHENG Lei, YANG Ping, SHEN Caihong, LIU Xiaogang
    China Brewing. 2026, 45(5): 127-136. https://doi.org/10.11882/j.issn.0254-5071.2026.05.016
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    Flavor compounds in 13 sauce-flavor Baijiu samples from different production areas of the Chishui River Basin were systematically investigated using multiple analytical techniques, including gas chromatography-mass spectrometry, gas chromatography-flame ionization detection, high-performance liquid chromatography, liquid chromatography-tandem mass spectrometry, and an automatic amino acid analyzer, combined with odor activity values (OAVs) and partial least squares-discriminant analysis (PLS-DA). In total, 146 flavor compounds were identified across all samples, comprising 49 esters, 23 alcohols, 13 acids, 14 aldehydes, 12 ketones, 10 pyrazines, 8 compounds from other categories, and 17 amino acids. Based on OAVs ≥ 1, 63 key aroma-active flavor compounds were further screened. Using these 63 key aroma-active compounds as classification variables, a discrimination model for sauce-flavor Baijiu from different production areas of the Chishui River Basin was established, and the PLS-DA results showed a clear separation trend among samples from different regions. Furthermore, 23 key differential flavor compounds were identified based on variable importance in projection (VIP) > 1, revealing their distribution patterns in sauce-flavor Baijiu from different production areas. This study provides methodological references and data support for quality evaluation and geographical origin traceability of sauce-flavor Baijiu.
  • CHEN Liang, ZHANG Jingjing, ZHU Liping, WU Yanhua, ZHANG Long, TANG Jie, ZHANG Gang, JIANG Wei, YANG Qiang, CHEN Shenxi
    China Brewing. 2026, 45(5): 137-143. https://doi.org/10.11882/j.issn.0254-5071.2026.05.017
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    This study comprehensively analyzed the microbial communities of five typical types of Xiaoqu (T5D, GLXQ, GLQQ, YXYQ, TFYQ) in Guangxi using high-throughput sequencing technology. The main physicochemical indicators were determined by conventional detection methods, and Pearson correlation analysis was applied to explore the correlations between microbial communities and physicochemical indicators. The results showed that among the samples, YXYQ had the highest moisture content, GLXQ had the highest acidity, and TFYQ exhibited the highest saccharifying power, glucoamylase activity, and α-amylase activity. The bacterial and fungal community structures varied among different Xiaoqu samples. In terms of bacterium, the relative abundances of Weissella in T5D, GLXQ, and GLQQ were 95.40%、31.08%, and 76.98%, respectively. YXYQ had a Lactobacillus relative abundance of 19.45%, while TFYQ was dominated by Bacillus at 82.42%. Regarding fungus, Wickerhamomyces relative abundances in GLXQ and GLQQ were 85.38% and 26.77%, respectively. T5D and YXYQ had Saccharomyces proportions of 24.48% and 21.92%, respectively, and TFYQ contained 37.77% Candida. Correlation analysis revealed that the acidity of Xiaoqu samples was significantly positively correlated with Wickerhamomyces and Sphingomonas (P < 0.01), while moisture content was significantly negatively correlated with Cyberlindnera and Candida (P < 0.01). Saccharifying power, amylase activity, and glucoamylase activity were positively correlated with Rhizopus, Cyberlindnera, and others. The findings of this study provide an important scientific basis for improving the quality and stability of Xiaoqu.
  • LI Xiaohong, LI Qiji, PAN Chengfeng, WANG Yu, PENG Mei, ZHANG Yinju, HUANG Nian, ZHANG Shunchang, YANG Juan
    China Brewing. 2026, 45(5): 144-151. https://doi.org/10.11882/j.issn.0254-5071.2026.05.018
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    This study administered varying doses of Ganoderma lucidum Baijiu to mice via intragastric gavage. The hepatoprotective efficacy of G. lucidum Baijiu against acute alcohol-induced liver injury was assessed by measuring liver indices, serum activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and malondialdehyde (MDA) content in liver tissue, along with histopathological examination of liver sections. In parallel, a network pharmacology approach was applied to construct a “component-target-pathway” network for topological analysis, aiming to identify key bioactive components and their targets, thereby elucidating the underlying molecular mechanisms. Results demonstrated that, compared with the model group, all dose groups of G. lucidum Baijiu significantly ameliorated liver indices in mice. MDA levels in the low-, medium-, and high-dose groups were markedly reduced by 29.58%, 39.79%, and 29.66%, respectively (P < 0.01). AST activity decreased significantly by 31.64%, 66.67%, and 60.54% (P < 0.01), while ALT activity was reduced by 60.44%, 76.06%, and 72.45% (P < 0.01), respectively. Histopathological analysis of liver tissue revealed improvements in alcohol-induced hepatic damage. Network pharmacology analysis suggested that triterpenes and sterols in G. lucidum Baijiu may attenuate liver injury by modulating oxidative stress, inflammatory responses, and hepatic metabolism. These results provide scientific evidence supporting the protective role of G. lucidum Baijiu in acute alcoholic liver injury.
  • SHAO Lei, LI Shuchai, WEI Shengbin, SHEN Gang, SHI Lihong, XU Feng, ZHU Jiahong, LIU Yong
    China Brewing. 2026, 45(5): 152-157. https://doi.org/10.11882/j.issn.0254-5071.2026.05.019
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    Using Jiuqu of Congjiang Weijiu as research objects, the microbial community diversity and function of Jiuqu was analyzed by Illumina MiSeq high-throughput sequencing technique. The potential metabolic pathways and core enzymes mediated by microorganisms in the Jiuqu were further predicted and annotated based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Carbohydrate-Active enZYmes (CAZy) databases, respectively. The results showed that the dominant phyla in Jiuqu of Congjiang Weijiu included Mucoromycota, Ascomycota, Bacillota, and Pseudomonadota. The predominant genera were Rhizopus, Aspergillus, Saccharomycopsis, and Mucor. Rhizopus arrhizus, Rhizopus delemar, Rhizopus microsporus and Saccharomycopsis crataegensis were predominant species. The microorganisms in Congjiang WeiJiu Jiuqu were mainly involved in carbohydrate metabolism, amino acid metabolism, nucleotide metabolism and energy metabolism. The core carbohydrate enzymes identified in Jiuqu were glycoside hydrolase, α-amylase, chitinase, and glycosyltransferase. The results provided a theoretical basis for conducting studies on the Weijiu Jiuqu-making and the optimization of its flavor-related processes.
  • WANG Xinye, TAO Renli, TAN Xiaoting, LUO Zhenbiao, LI Hongxia, MIN Zhuo, YUE Qianqian, ZHAO Liang
    China Brewing. 2026, 45(5): 158-162. https://doi.org/10.11882/j.issn.0254-5071.2026.05.020
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    To explore the diversity and functional potential of fungal communities in the surface of straw covering sauce-flavor Daqu, straw samples were collected from Renhuai, Guizhou Province. Fungal isolates were isolated and identified via dilution-plate method combined with molecular biological technologies, and their environmental tolerance, enzyme-producing capacity and volatile metabolites were further analyzed. The results showed that a total of eight fungal strains were isolated and identified, including Paecilomyces variotii (DC-9, DC-21), Aspergillus spinulosporus (DC-16, DC-36), Penicillium citrinum (DC-23), Saccharomycopsis fibuligera (DC-27), Cladosporium xanthochromaticum (DC-28) and Cladosporium cladosporioides (DC-39). Among them, strain DC-21 exhibited excellent environmental tolerance, which could tolerate temperature 40 ℃, lactic acid concentration 80 g/L, pH 5, and ethanol volume fraction 8%. Strains DC-28 and DC-39 possessed prominent enzyme-producing ability, both capable of synthesizing two types of enzymes, with strain DC-28 producing cellulase and tannase, and strain DC-39 producing cellulase and α-amylase. A total of 22 volatile metabolites were detected in the fermentation broths of the eight strains; strains DC-27 and DC-28 produced the most volatile metabolites, with 18 compounds detected in each strain. In conclusion, the fungi in the surface of Daqu-covered straw have excellent tolerance to brewing stress conditions, enzyme production performance and aroma-enhancing property. This study provides valuable microbial resources for optimizing the microecology of Daqu and strengthening the fermentation process of sauce-flavor Baijiu.
  • Maidina·PAERHATI, FENG Zongtao, YIN Xiu, ZHANG Erhao, LAN Xiaozhong, LU Yazhou
    China Brewing. 2026, 45(5): 163-170. https://doi.org/10.11882/j.issn.0254-5071.2026.05.021
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    To investigate the effect of production region and cultivar on the epiphytic microorganisms of wolfberry fruits, using fruit samples of three wolfberry types as research objects: Lycium barbarum from Ningxia (NZ), L. barbarum from Qinghai (QR), and Lycium ruthenicum (QB), the community structure characteristics and diversity of epiphytic microorganisms on the fruit surfaces of wolfberry from different production regions and cultivars were analyzed with high-throughput sequencing technology. The results showed that the fruit surface of sample NZ had the highest diversity and richness of both bacteria and fungi, and the community structures of bacteria and fungi were more susceptible to geographical origin. A total of 3 dominant bacterial phyla and 12 dominant bacterial genera, as well as 2 dominant fungal phyla and 14 dominant fungal genera (relative abundance > 1.00%), were identified across all samples. Pantoea was the shared dominant bacterial genus, showing an absolute predominance in sample QB with a relative abundance of 97.93%. The shared dominant fungal genera were Alternaria and Cladosporium, which exhibited the highest relative abundances in samples NZ and QB with values of 23.85% and 83.60%, respectively. Functional prediction of bacterial communities based on the Clusters of Orthologous Genes (COG) database demonstrated that amino acid transport and metabolism constituted the primary functional category. Functional annotation of fungal communities using the Fungi Functional Guild (FUNGuild) showed that saprotrophic and pathogenic functions were predominant. In conclusion, both geographical origin and cultivar could shape the composition and functional profiles of microbial communities on the epidermis of wolfberry fruits.
  • FENG Menghan, HU Jiaxing, BI Jinfeng, ZHANG Aidong, WANG Liping, LI Xuan, YANG Yang
    China Brewing. 2026, 45(5): 171-180. https://doi.org/10.11882/j.issn.0254-5071.2026.05.022
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    In this study, Xinjiang ‘Hotan Hong’ grapes were used as raw material to prepare dry wine, semi-sweet wine (both fermented wines), fortified wine, as well as grape distilled spirits with ethanol volume fractions of 52% and 76%. Basic physicochemical indexes, functional components, mineral elements and volatile flavor compounds were analyzed respectively. The results showed that the contents of functional components (total phenols, total flavonoids, tannins and total flavanols) and mineral elements in dry, semi-sweet and fortified wines were higher than those in the two grape distilled spirits. The 76% distilled spirit possessed the richest types of volatile flavor compounds (44) and the highest total content (1 726.47 μg/L). Combined with odor activity value (OAV), a total of 8 key aroma compounds (OAV ≥ 1) were identified in the five wines. Ethanol, isobutanol and ethyl hexanoate were the common key aroma substances; isoamyl alcohol, methyl decanoate and ethyl 9-decenoate were the unique key aroma substances of distilled spirits; and 1-nonanol and nonanal were unique to fortified wine. Correlation analysis showed that volatile flavor compounds (except acids) were positively correlated with each other. Zn, Mn, P, K, Na and Ca promoted the synthesis of esters, and Mg facilitated the formation of terpenoids. This study clarified the quality differences of wines produced from ‘Hotan Hong’ grapes under different brewing processes, and provided theoretical support for the process optimization and product development of ‘Hotan Hong’ wine.
  • WU Jieying, CHEN Huimei, LAN Yanbin, WANG Ali, HONG Tingting, HUANG Guidong, ZHONG Xianfeng
    China Brewing. 2026, 45(5): 181-187. https://doi.org/10.11882/j.issn.0254-5071.2026.05.023
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    To systematically explore the lactic acid bacteria strain resources, lactic acid bacteria were isolated from homemade vinegar by traditional culture methods. Their phenotypic characteristics were identified by morphological observation, and species classification was determined by physiological and biochemical tests combined with molecular biotechnology. The safety of the strains was evaluated based on hemolytic activity, indole test and antibiotic sensitivity. The results indicated that a total of 14 strains of bacteria were isolated from homemade vinegar in this study. All the isolated strains were Lactiplantibacillus plantarum. Among these, 13 strains were identified as L. plantarum subsp. plantarum, and 1 strain was identified as L. plantarum subsp. argentoratensis. All 14 strains showed α-hemolysis, and their indole test results were negative. In the antibiotic sensitivity tests, 14 strains of bacteria were found to be sensitive or moderately sensitive to five antibiotics including ampicillin, but were resistant to norfloxacin, 13 strains were resistant to gentamicin and vancomycin. In summary, the 14 strains isolated from homemade vinegar possed certain safety properties, providing strain resources and basic data for their development and application in the food processing industry.
  • GUO Yanru, HAO Linlin, YAO Huimin, WU Baomei, LIU Weizhong, WANG Binbin
    China Brewing. 2026, 45(5): 188-194. https://doi.org/10.11882/j.issn.0254-5071.2026.05.024
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    Exopolysaccharide (EPS)-producing lactic acid bacteria were isolated from 13 types of pollen using the dilution plating and three-zone streak plate methods. The isolated strains were subjected to morphological observation, molecular biological identification, and physiological-biochemical characterization. Their tolerance to acid, bile salts, urea, and NaCl was evaluated. The results indicated that one EPS-yielding strain, designated YF, was isolated from Robinia pseudoacacia pollen. The EPS yield of this strain was (5.213 5 ± 0.034 5) g/L. Strain YF was identified as Apilactobacillus kunkeei, a Gram-positive bacterium. This strain demonstrated the ability to extensively utilize eight different carbon sources, including sucrose, fructose and rhamnose. Its optimal growth time was 21 h, and it exhibited strong environmental resilience. The optimal conditions for its growth were a pH of 6, an NaCl content of 2% (m/m), a urea content of 2% (m/m), and a porcine bile salt content of 0.4% (m/m). This study lays a theoretical foundation for expanding the probiotic resource library and developing functional EPS products.
  • YU Zhuojun, ZENG Yaoying, HUO Hongyan, ZHANG Kai, SHE Jiehai, ZHOU Wenhua
    China Brewing. 2026, 45(5): 195-201. https://doi.org/10.11882/j.issn.0254-5071.2026.05.025
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    To investigate regional variations in the quality of pickled mustard within Huarong County, total acid, soluble solids, vitamin C and other physical and chemical indexes of pickled mustard in five towns of Huarong County were determined. Correlation analysis, cluster analysis (CA), principal component analysis (PCA), and partial least squares-discriminant analysis (PLS-DA) were used for comprehensive evaluation and difference analysis. The results showed that Yushan Town samples exhibited the highest levels of total acid ((18.24 ± 0.16) g/kg), amino-acid nitrogen ((0.26 ± 0.01) g/100 g), total soluble sugars ((1.32 ± 0.01) g/100 g) and total phenolics ((2.43 ± 0.02) mg/g) among all towns. Samples from Zhihedu Town were richest in soluble solids ((15.52 ± 0.08)%) and vitamin C ((336.33 ± 8.70) mg/kg) and Chaqi Town samples were richest in dry matter ((15.72 ± 0.44)%)) and crude fiber contents ((15.53 ± 0.34) g/kg). Correlation analysis showed that the total acid content exhibited an extremely significant positive correlation with amino acid nitrogen, total soluble sugar, crude fiber and total phenols (P < 0.01), and the salt content had an extremely significant negative correlation with the contents of amino acid nitrogen, total soluble sugar and total phenols (P < 0.01). CA, PCA, and PLS-DA collectively classified the pickled mustard into three distinct groups: Yushan Town samples were associated with superior nutritional quality; those from Chaqi and Zhihedu towns were distinguished by enhanced chewiness; and samples from Chengguan and Sanfengsi towns were characterized by milder acidity. The integrated scores ranked the towns in descending order as follows: Yushan > Zhihedu > Chaqi > Chengguan > Sanfengsi.
  • SUN Jinghui, ZHANG Xiaoxi, LI Yaole, ZHANG Wang, TAN Jingxi, HAN Fuliang
    China Brewing. 2026, 45(5): 202-208. https://doi.org/10.11882/j.issn.0254-5071.2026.05.026
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    To investigate the effects of wine standing conditions on aroma evaluation, the influence of glass shape (Bordeaux glass vs. Burgundy glass), volume (350, 500, 650 mL), and wine standing time (0, 15, 30 min) on the aroma quality and characteristics of dry white was analyzed. The results indicated that glass shape, volume, and wine standing time all influenced dry white wine aroma evaluation. Both the 350 mL Bordeaux glass and 350 mL Burgundy glass achieved the optimal aroma quality, with the highest scores for intensity, elegance, typicality and sensory evaluation: 7.92, 7.58, 7.58 and 17.54 for the Bordeaux glass; and 7.67, 7.50, 7.54 and 17.42 for the Burgundy glass. The 350 mL Bordeaux glass yielded the best aroma quality when samples were standing for 15 min, scoring 7.25 for intensity, 7.50 for elegance, 7.50 for typicality, and 17.33 for sensory evaluation, fully presenting floral and fruity characteristics. For the 350 mL Burgundy glass, the highest aroma quality was achieved immediately after pouring (0 min), with scores of 7.42 for intensity, 7.42 for elegance, 7.08 for typicality, and 17.42 for overall sensory evaluation, presenting a refreshing fruity aroma profile. In summary, the 350 mL Burgundy glass is the recommended for the aroma evaluation of dry white wines.
  • ZHANG Wanxue’er, CHEN Yingjie, LIU Xiuhai, ZHANG Haijun, HE Jing, YANG Xingyuan
    China Brewing. 2026, 45(5): 209-215. https://doi.org/10.11882/j.issn.0254-5071.2026.05.027
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    Using Marselan grape variety as raw material, dry red wine was produced via fermentation with four Saccharomyces cerevisiae strains (XR, XPURE, F15, and CECA). Basic physicochemical properties and volatile aroma profiles were characterized using routine analytical methods and headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS), while sensory evaluation was performed to clarify the impacts of different yeast strains on overall wine quality. The results indicated that the four wine samples exhibited slight differences in physicochemical parameters, and all of which met relevant national standard (GB 15037-2006) requirements. A total of 88 volatile aroma compounds were detected in the Marselan dry red wines produced with different wine yeasts, including 24 alcohols, 41 esters, 12 organic acids, 8 terpenes, 2 ketones, and 1 phenolic compound; esters and alcohols dominated in relative content. A total of 56 volatile components were shared by all wine samples. Based on relative odor activity value (ROAV), three common characteristic aroma substances with ROAV > 10 were screened: ethyl caproate, ethyl hexanoate, and damascenone. Principal component analysis (PCA) further verified significant discrepancies in volatile flavor compositions among the four batches. Sensory evaluation revealed distinctive organoleptic features for each sample. Notably, the wine fermented by strain F15 achieved the highest sensory score of 91, presenting superior performance in appearance, aroma complexity, mouthfeel and varietal typicality compared with the other three fermented groups.
  • SU Hao, LIN Mei, REN Xiangyun, LIANG Zhangcheng, XU Jinglan, XU Changyi, LI Weixin
    China Brewing. 2026, 45(5): 216-222. https://doi.org/10.11882/j.issn.0254-5071.2026.05.028
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    To enhance the quality of Vitis davidii wine, using Ziqiu grape as the raw material and Saccharomyces cerevisiae JP2 as the fermentation strain, the V. davidii wine was prepared using a juice-separated (saignee) fermentation method, and the effects of different juice separation ratios (0%, 10%, 15%, 20%, 25%, 30%, 35%) on the basic physicochemical indicators, phenolic compounds, sensory quality, and volatile flavor components of wine were investigated. The results indicated that the optimal juice-separated ratio for V. davidii wine fermentation was 30%. Under the condition, the total sugar content of wine was 3.52 g/L, the total acid content was 7.67 g/L, and the alcohol content was 12.70%, classifying it as a dry red wine. Compared to wine produced without juice separation, the total phenol, proanthocyanidin, anthocyanin, total flavonoid, and tannin contents increased by 28.02%, 81.65%, 66.38%, 47.75%, and 208.68%, respectively. Both red and yellow chroma increased, resulting in higher color saturation and a larger color difference, presenting a brick red color. The aroma became more intense, and the wine body and taste were more harmonious. The contents of esters, alcohols, acids, aldehydes and ketones, and other substances increased by 37.86%, 9.45%, 7.92%, 44.14%, and 135.77%, respectively. There were 12 volatile aroma compounds with an odor activity value (OAV) > 1, among which ethyl caproate, isoamyl acetate, ethyl caprylate, ethyl decanoate, and eugenol were all present in higher concentrations in wine produced with juice separation 30% than the juice without separation. In summary, juice-separated fermentation can enhance the quality of Ziqiu grape wine.
  • ZHENG Haiwu, LEI Lei, ZHANG Meizhi, LI Zhengying, XIN Shengbing, ZHAO Xueping, SU Guirong
    China Brewing. 2026, 45(5): 223-228. https://doi.org/10.11882/j.issn.0254-5071.2026.05.029
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    To screen non-Saccharomyces strains that can synergistically improve the quality of wine with the indigenous Saccharomyces cerevisiae WJ1, five high-yielding ester non-Saccharomyces strains (33286, 33172, 33257, 33194, 33287) were used as research objects. Their stress tolerance and enzyme-producing capacity were evaluated, and excellent strains were screened through mixed fermentation tests and sensory quality analysis. The results showed that strains 33286 and 33194 exhibited superior tolerance, capable of tolerating alcohol 15%, citric acid 35 g/L, glucose 250 g/L, and SO2 300 mg/L. They also presented high activities of protease (32.16 and 27.21 U/mL) and β-glucosidase (34.5 and 27.1 U/mL). In mixed fermentation, the ester yields of WJ1 co-fermented with strains 33286 and 33194 reached 6.28 and 6.82 g/L, respectively, which were significantly higher than that of WJ1 single fermentation (3.93 g/L). Sensory evaluation indicated that the wine fermented by WJ1 and strain 33194 achieved the highest sensory score of 88. In conclusion, non-Saccharomyces 33194 is an excellent candidate strain for synergistic fermentation with indigenous S. cerevisiae WJ1 to improve wine quality. It can be applied in the optimization of characteristic wine fermentation processes and high-quality wine production, providing excellent strain resources and a theoretical basis for the exploitation of indigenous yeast resources and the improvement of wine flavor.
  • GU Wenjian, MA Yuan, HOU Shuheng, MA Zihao, ZHAO Yi, WEI Yufei, GAO Yueyue, CHANG Di, ZHUANG Yuan, GAO Hongbo
    China Brewing. 2026, 45(5): 229-236. https://doi.org/10.11882/j.issn.0254-5071.2026.05.030
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    To explore the effects of different fermentation periods on the flavor and quality of light-flavor Baijiu, sensory evaluation was conducted on light-flavor Baijiu with fermentation periods of 28 and 56 days. Gas chromatography and liquid chromatography were used to determine volatile flavor substances and non-volatile lactic acid, and partial least squares-discriminant analysis (PLS-DA) was applied to analyze the differences in their contents. The results showed that 31 compounds were detected in the two Baijiu samples, including 8 alcohols, 5 aldehydes, 6 acids, and 12 esters. Ethyl acetate, ethyl lactate, isoamyl alcohol, n-propanol, etc., were the main substances of light-flavor Baijiu. The sample fermented for 56 days had a more intense aroma (such as floral and fruity aromas), a softer and more coordinated taste, and was mellow, sweet, and had a unique and typical flavor. The contents of sec-butyl alcohol, isobutyl alcohol, isoamyl alcohol, acetaldehyde, furfural, ethyl butyrate, and lactic acid in this sample were significantly higher than those in the sample fermented for 28 days (P < 0.05). The odor activity values (OAVs) of 22 substances in both samples fermented for 28 and 56 days were greater than 1. The results of PLS-DA showed that there were 13 differential substances with variable importance in projection (VIP) > 1 in the Baijiu samples with different fermentation periods, including n-hexanal, sec-butyl alcohol, isobutyl alcohol, etc. The correlation results between sensory properties and flavor compounds showed that furfural was highly significantly correlated with fullness (P < 0.001), and there was a positive and extremely significant correlation with the fragrance, floral aroma, and fruity aroma (P < 0.01). In conclusion, extending the fermentation period of light-flavor Baijiu can improve the sensory properties and quality of the liquor body.
  • YUAN Mengqin, LIU Feng, ZHENG Yifan, LAN Zhentao, WANG Zixuan, LI Rong
    China Brewing. 2026, 45(5): 237-241. https://doi.org/10.11882/j.issn.0254-5071.2026.05.031
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    Using sweet corn as the main raw material, sweet corn Jiaosu was prepared by mixed fermentation (1:1) of Saccharomyces cerevisiae and Lactiplantibacillus plantarum. The effects of inoculation amount of mixed fermentation strains, addition amount of white granulated sugar, fermentation temperature, and time on the superoxide dismutase (SOD) activity in the sweet corn Jiaosu were first investigated through single factor test. The optimal production parameters were then determined by orthogonal testing, and the in vitro antioxidant activity was determined. The optimal process condition for the Jiaosu fermentation is as follows: the inoculum 0.7%, the addition of white granulated sugar 200 g/100 mL, the fermentation temperature 40 ℃, and the fermentation time 2 days. The SOD activity was (58.47 ± 1.08) U/mL under the optimized condition. At a dose of 20.0 mg/mL, the scavenging rates against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radical and hydroxyl radical were 81.2%, 88.2% and 69.1%, respectively. In conclusion, the sweet corn Jiaosu exhibited favorable antioxidant activity and possessed promising potential for the development and application of health-oriented food products.
  • GU Junhua, XING Xiaoke
    China Brewing. 2026, 45(5): 242-249. https://doi.org/10.11882/j.issn.0254-5071.2026.05.032
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    To fully utilize the fermentation potential of the strains for further enhancing the quality of cherry fruit wine, a co-fermentation strategy between non-Saccharomyces yeast extracellular extracts and Saccharomyces cerevisiae was constructed in this study. The effects of the extracellular extracts of four non-Saccharomyces yeast strains, including Pichia kudriavzevii, Hanseniaspora uvarum, Issatchenkia orientalis, and Schizosaccharomyces pombei, on the conventional physicochemical properties, color characteristics, phenolic substances and antioxidant activities of cherry fruit wine were investigated. The results showed that the co-fermentation of non-Saccharomyces yeast extracellular extracts with S. cerevisiae improved the color and physicochemical properties of cherry fruit wines, as well as increased the contents of total flavonoids, total polyphenols, and total anthocyanins, and enhanced the antioxidant activity, which was most prominent with the extracellular extracts of P. kudriavzevii. Compared with the fermentation with S. cerevisiae alone, the co-fermentation of cherry fruit wine with P. kudriavzevii extracellular extract and S. cerevisiae had increased the ethanol, vitamin C and total ester content by 8.52%, 5.13%, and 8.69%, and the contents of total flavonoids, total polyphenols, and total anthocyanins were increased by 21.78%, 14.27%, and 33.50%, and the scavenging rates of 1,1-diphenyl-2-picrylhydrazyl radical, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical, and hydroxyl radical were enhanced by 20.09%, 14.18% and 15.59%, respectively. Hence, the co-fermentation of non-Saccharomyces yeast extracellular extracts with S. cerevisiae could effectively improve the qualities and health benefits of cherry fruit wine.
  • ZHAO Yushun, WANG Xueqing, FENG Lihong, ZHANG Tinggang, Roshengul·YUNUS, XU Hongtao, REN Xiaona
    China Brewing. 2026, 45(5): 250-257. https://doi.org/10.11882/j.issn.0254-5071.2026.05.033
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    To enrich the variety of naan products, red jujube-milk naan was made using flour, milk and red jujube as the main raw materials. With sensory evaluation score and specific volume as evaluation indices, the formula of red jujube-milk naan was optimized by single-factor and orthogonal experiments, and the differences in main nutritional components and volatile flavor substances between it and two commercially available milk naans (A and B) were compared and analyzed. The results showed that the optimal formula for red jujube-milk naan, based on 100 g of flour, was as follows: milk 47.5%, yeast and baking soda (1:1, m/m) 0.8%, butter 12%, and dried red jujube paste 20%. Under the condition, the sensory score of red jujube-milk naan was 96.10, and the specific volume was 2.32 mL/g. Its main nutritional components were the same as those of commercially available milk naans, but the coefficient of variation (CV) of water content was relatively larger, at 38.80%, followed by carbohydrates and ash, with CVs of 8.97% and 7.67%, respectively. A total of 64 kinds of volatile flavor substances were detected, 35, 36 and 34 kinds of volatile flavor components were detected in red jujube-milk naan, commercially available milk naans A and B, respectively, and 11 kinds of volatile flavor substances were shared by the three kinds of naan. Red jujube-milk naan had significantly different volatile flavor substances from commercially available milk naans A and B, containing characteristic flavor substances such as n-nonanal, n-heptanal, and n-octanal, with richer aroma substances. The results of this study can provide certain references for the development of naan products.
  • WANG Yue, CHEN Chong, ZHENG Zhaojun, LI Pinhe, LI Dongliang, JI Xiaoying
    China Brewing. 2026, 45(5): 258-265. https://doi.org/10.11882/j.issn.0254-5071.2026.05.034
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    Cigar tobacco leaves were fermented using strong-flavor Baijiu, Saccharomyces cerevisiae cell suspension, its supernatant, and ethanol as fermentation media. The volatile flavor compounds in cigar tobacco leaves fermented with different media were determined with headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry, aiming to explore the specific pathways of ester synthesis by S. cerevisiae. Non-ester differential volatile flavor compounds were screened by principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA), and variable importance in projection (VIP), and sensory evaluation of the fermented tobacco leaves was conducted. The results showed that the content and variety of ester compounds in cigar tobacco leaves fermented with S. cerevisiae fermentation supernatant combined with ethanol were significantly increased compared to the original tobacco leaves (P < 0.05). Among them, ethyl acetate (0.002 5 μmol/g) and ethyl hexadecanoate (0.002 5 μmol/g) were significantly enriched. The enhancement mechanism was attributed to exogenous esterase catalysis, ethanol-mediated substrate migration, and hydrophobic retention effects. This fermentation medium had no significant impact on key aroma components such as dihydroactinidiolide in cigar tobacco leaves. A total of 47 non-ester differential volatile flavor compounds, including phenethyl alcohol and n-hexanal, were screened. Sensory evaluation indicated that the aroma intensity, maturity, and other indicators of cigar tobacco leaves in the S. cerevisiae fermentation supernatant-ethanol co-fermentation group were significantly improved. This study provides microbial resources for developing cigar flavor-enhancing technology based on in-situ microbial fermentation.
  • ZHOU Yang, HU Weiwei, WU Weicheng, LU Yanhui, XU Guangzhi, ZHANG Youzuo, WANG Yan
    China Brewing. 2026, 45(5): 266-272. https://doi.org/10.11882/j.issn.0254-5071.2026.05.035
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    To systematically evaluate the nutritional components and thermal processing characteristics of 26 different sorghum varieties, the nutritional components of the sorghum, and the pasting properties and thermal characteristics of the sorghum starch were determined by conventional analytical methods, rapid viscosity analyzer, and differential scanning calorimetry, respectively. A comprehensive evaluation model for the thermal processing quality of sorghum was then developed by integrating correlation analysis and principal component analysis (PCA). The results revealed significant variations in both nutritional composition and thermal processing properties among the different sorghum varieties. Regarding nutritional composition, the contents of amylose (1.29%-19.07%) and total phenolics (0.41%-0.88%) showed considerable differences. In terms of the thermal processing properties of sorghum starch, significant variations were observed in setback viscosity (110-813 cP) and trough viscosity (852-1 572 cP). Correlation analysis indicated that total starch, amylopectin, peak viscosity, setback viscosity, and onset gelatinization temperature served as the primary indicators for predicting the thermal processing characteristics of sorghum. PCA results demonstrated that the cumulative variance contribution rate of the first six PCs reached 84.27%, which provided a basis for constructing a comprehensive sorghum quality evaluation model. Application of this model to assess the 26 sorghum varieties identified Heike Baitengguai, Hongyingzi 1669, and Hongliangfeng 1 as exhibiting superior quality. The findings of this study offer a theoretical foundation for elucidating the distinctive characteristics and potential processing applications of different sorghum varieties.
  • DAI Yuanyuan, MENG Fei, XIE Xiaohua, CHEN Jing, HU Xiaopei
    China Brewing. 2026, 45(5): 273-281. https://doi.org/10.11882/j.issn.0254-5071.2026.05.036
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    To investigate the effects of different substrate raw materials (milk powder, milk powder + Euryale ferox powder, E. ferox powder) on the volatile flavor components of the fermented milk produced by them, the milk-based fermented milk, milk with E. ferox-based fermented milk and E. ferox-based fermented milk were analyzed using sensory evaluation combined with gas chromatography-ion mobility spectrometry (GC-IMS) technology. Fingerprint chromatogram comparison analysis, hierarchical cluster analysis (HCA), principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were conducted on volatile flavor components. The results showed that E. ferox-based fermented milk achieved the highest sensory score (92.50 ± 1.08). A total of 51 volatile flavor components were detected in the three types of fermented milk, including 12 ketones, 14 alcohols, 11 aldehydes, 5 esters, 2 acids, and 7 others. Significant differences in volatile flavor components were revealed by fingerprint profiles, HCA and PCA among the three fermented milk samples. The three types of fermented milk could be effectively distinguished by PCA and OPLS-DA. A total of 18 key differential components were screened out through variable importance in projection (VIP) > 1 and P < 0.05, including 7 ketones, 3 alcohols, 4 aldehydes, 2 pyrazines, 1 acid, and 1 ester. This study provides a scientific basis for analyzing the volatile aroma characteristics of fermented milk produced from different substrate materials.
  • ZHANG Baochun, SU Chenxue, CHE Jia, WANG Yue, SHEN Chunhua, YU Lina, SUN Xiangyu, LIU Wenzheng, FANG Yulin
    China Brewing. 2026, 45(5): 282-289. https://doi.org/10.11882/j.issn.0254-5071.2026.05.037
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    Brandy is a high-proof distilled spirit made from grapes or other fruits through fermentation, distillation, and oak barrel aging, and it is widely appreciated for its unique flavor and long history. This study, based on bibliometric methods and using CiteSpace software, conducted statistical and visualization analyses of brandy-related literature from the China National Knowledge Infrastructure (CNKI) and Web of Science (WOS) core collection between 1985 and 2024 (947 Chinese publications and 758 English publications). The results showed that the annual publication volume on brandy has generally increased since 1990; the main contributing countries were Spain, the United States, and Portugal; and the most prolific journals were the Journal of Agricultural and Food Chemistry and Liquor-Making Science & Technology. Among Chinese core authors, Zhang Baochun, Li Jiming, and Zhao Yuping maintain close collaboration, while Canas S is the most prolific author in the WOS core collection. Institutional analysis indicated that international brandy research was mainly concentrated in European institutions, with Portuguese institutions contributing the largest number of publications and forming cross-national collaborative networks; domestically, research was primarily conducted by liquor enterprises and universities, with Yantai Changyu Group in Shandong making significant contributions and forming a regionally close-knit collaboration network. Keyword co-occurrence analysis reveals that current research hotspots focus on aroma component analysis. This study provides a systematic review of the literature, revealing trends and the evolution of research hotspots in the field of brandy, offering a reference for future academic research.
  • QIU Min, WANG Kai, TONG Jun
    China Brewing. 2026, 45(5): 290-295. https://doi.org/10.11882/j.issn.0254-5071.2026.05.038
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    In this study, listed Chinese wine enterprises were selected as the research sample, and the relationship between intellectual property (IP) information disclosure and brand value was examined based on information asymmetry theory. Using an expert scoring method, annual report disclosure texts of 10 listed wine companies from 2014 to 2023 were quantitatively analyzed. The empirical results indicated that the level of corporate IP information disclosure was significantly and positively associated with brand value, meaning that higher disclosure levels correspond to greater brand value. Based on these findings, recommendations were proposed at the government, market regulatory, and enterprise levels to enhance the IP information disclosure practices of listed companies and to promote the healthy development of the wine industry.
  • LI Jie, LIU Yanhong, LI Tianran
    China Brewing. 2026, 45(5): 296-303. https://doi.org/10.11882/j.issn.0254-5071.2026.05.039
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    Adopting new-quality productive forces as the core perspective, taking Luzhou City, Sichuan Province as a typical case, the internal mechanisms and practical paths of new-quality productive forces driving the integration of Baijiu culture and tourism were systematically explored. Through theoretical deduction, empirical research, and case analysis, a theoretical model for the integrated development of Baijiu culture and tourism was constructed, with technological innovation, institutional innovation, and management innovation as the three constituent elements. The research finded that technological innovation achieved precise resource allocation and experience upgrading through digital and intelligent means, serving as the core engine for enhancing industrial competitiveness. The cross-departmental coordination barriers formed by lagging institutional innovation were the key crux of insufficient integration depth; management innovation provides important support for efficient industrial operation by optimizing benefit distribution and resource allocation. The study proposed a trinity practical path system of “technological empowerment, model reconstruction, and management optimization”, forming a replicable and scalable “Luzhou solution”. The research not only provided theoretical support for industrial culture and tourism integration in the context of new productive forces, but also offered practical guidance for the transformation and upgrading of Baijiu-producing regions and even traditional industries nationwide, possessing significant theoretical and practical value.
  • SONG Baoli, YANG Huili, WANG Yu, WANG Hao
    China Brewing. 2026, 45(5): 304-309. https://doi.org/10.11882/j.issn.0254-5071.2026.05.040
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    The digital transformation of the supply chain can help Baijiu enterprises better focus on customers and stand out in the competition. Based on the review of relevant literature, this paper first analyzed the “bottlenecks” and “obstacles” of the traditional Baijiu supply chain. Then, it explored the value creation of the digital Baijiu supply chain from four aspects: breaking information silos, enhancing the stickiness of partnerships; maintaining relationship transparency, promoting cost reduction and efficiency improvement for Baijiu enterprises; timely obtaining demand information, precisely reaching customers; and achieving full-process tracking of production and brewing to stabilize product quality. Furthermore, it proposed the advancement path for the construction of the digital Baijiu supply chain, which included consolidating the digital foundation, promoting the digital transformation of each link in the supply chain, realizing digital quality control of Baijiu products, strengthening the full lifecycle management of equipment, enhancing the digital leadership of managers, and promoting organizational and cultural changes based on digitalization. This paper provided theoretical support and practical guidance for Baijiu enterprises to build a digital supply chain.