25 December 2025, Volume 44 Issue 12
    

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  • DIAO Yiao, ZHANG Yongqing, AO Ling, SUN Xiaotao, DONG Wei
    China Brewing. 2025, 44(12): 1-7. https://doi.org/10.11882/j.issn.0254-5071.2025.12.001
    Abstract ( ) Download PDF ( ) HTML ( )   Knowledge map   Save
    With the increasing improvement in living standards and the growing pursuit for a better life, the market demand for aged Baijiu with long aging years is gradually increasing. However, the traditional natural aging process is characterized by long cycle, high cost, and low efficiency, which significantly restricts the industry's supply capacity. Based on this, the Baijiu aging mechanisms and the dynamic evolution of flavor compounds during the aging process of different flavor types Baijiu were summarized, with a particular focus on the molecular mechanisms underlying natural aging and artificial aging techniques. On this basis, the effects of microenvironments of storage container on aging effect were discussed, the difference of the role of clay jars and stainless-steel tanks in the Baijiu aging process were compared, and the advantages and limitations of various artificial aging methods were evaluated, aiming to explore a simple, efficient and controllable aging strategy. The paper aiming to deepen the understanding of Baijiu aging processes and mechanisms, promote the scientific development of artificial aging technologies, and provide theoretical support and practical guidance for the standardization and industrialization upgrading of Baijiu aging technology.
  • YANG Wen, WANG Zhe, LIU Taotao, SHAN Yimin, TANG Jie, CAO Hong, LIU Shenghua, ZHAO Run, LI Qiang, HUANG Xiuhua
    China Brewing. 2025, 44(12): 8-12. https://doi.org/10.11882/j.issn.0254-5071.2025.12.002
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    With the popularization of the concept of healthy drinking, Lujiu has risen to become the third most popular type of alcoholic drink in China due to its combination of flavor and efficacy. The drinking comfort has become a key factor in determining market acceptance. The Baijiu industry has established a relatively complete drinking comfort evaluation system, while there are few studies on the drinking comfort evaluation methods and quality improvement of Lujiu. In this paper, the evaluation methods for the drinking comfort of Baijiu were summarized. Combined with the characteristics of Lujiu, a method suitable for evaluating the drinking comfort of Lujiu was proposed. The methods to improve the drinking comfort of Lujiu were expounded from three aspects: regulation of flavor substances of liquor base, addition of herbal extracts, and optimization of production processes. With the aim of providing a basis for Baijiu enterprises, high-quality Lujiu products were developed, to promote industrial standardization.
  • HU Jialiang, ZHANG Yang, WANG Xiaomin, YANG Huafeng, JIN Guojie
    China Brewing. 2025, 44(12): 13-20. https://doi.org/10.11882/j.issn.0254-5071.2025.12.003
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    Medium-chain fatty acid ethyl ester are key ester substances that affect the floral, fruity and other aroma characteristics of wine. Their low sensory threshold enables a slight change in concentration to significantly alter the sensory quality of wine, and their weak volatility can make the aroma of wine more lasting. In this paper, the biosynthesis mechanism of ethyl medium-chain fatty acid ester and their regulation and control mechanism during alcohol fermentation, malolactic fermentation and aging process of wine were systematically reviewed. The research progress of multiple regulatory strategies such as microorganism co-inoculation, fermentation parameters regulation and molecular biological modification were mainly discussed. Finally, the future research directions and trends were prospected, with the aim of providing a theoretical basis for the precise regulation of medium-chain fatty acid ethyl ester in wine, and ultimately enhancing the sensory quality and market competitiveness of wine.
  • CHEN Xingxing, BAO Aiming, AO Wenmin, LONG Zhengyu, LI Binbin, QIN Weijun
    China Brewing. 2025, 44(12): 21-27. https://doi.org/10.11882/j.issn.0254-5071.2025.12.004
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    Fermented sauerkraut, rich in carbohydrates and produced using large volumes of water as the fermentation substrate, is highly susceptible to spoilage by contaminating spoilage microorganisms. In China, the majority of fermented sauerkraut products rely on natural fermentation, which is often characterized by poor process stability and elevated nitrite levels, posing significant food safety concerns. Additionally, with accelerating urbanization and the faster-paced modern lifestyles, fermented sauerkraut is gradually trending towards smaller packaging. However, this trend has also introduced new challenges, such as the abuse of preservatives, browning during transportation, gas expansion in packaging, and the development of off-flavors. These issues make it difficult to eliminate the shortcomings of fermented sauerkraut, such as its food safety and short shelf-life, which seriously restricts the development of the fermented sauerkraut industry. In this paper, the current food safety issues associated with fermented sauerkraut were reviewed, the recent research advances in the screening and application of fermented sauerkraut strains were briefly introduced, and the existing packaging and sterilization technologies were expounded. Furthermore, it was proposed to optimize the fermentation process through inoculation technology to enhance fermentation stability and reduce food safety risks. From the perspective of sterilization and packaging technology, the feasibility of extending shelf-life and reducing preservative usage were also explored. The aim was to provide theoretical support for the standardized production and industry development of the fermented sauerkraut.
  • PAN Qianwen, TIAN Xiaoya, XU Weijie, CHEN Jingyu, ZHANG Xin
    China Brewing. 2025, 44(12): 28-33. https://doi.org/10.11882/j.issn.0254-5071.2025.12.005
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    The high occupancy rate and low utilization rate of low-grade tobacco leaf resources in China have been recognized as major challenges for the tobacco industry. Excessive content of macromolecular substances in tobacco leaves is one of the key factors leading to inferior quality of tobacco leaves. Using biotechnology to degrade these macromolecular substances, balance chemical components and increase aroma substances is one of the effective ways to improve the quality of low-grade tobacco leaves. In recent years, the application of enzyme technology and microbial technology in improving the quality of tobacco leaves has become a research hotspot in the industry. The influence of macromolecular substances such as starch, protein, pectin and cellulose in tobacco leaves on quality was introduced, and the mechanisms and related research progress of enzymes and microorganisms in tobacco fermentation were sorted out and summarized, aiming to provide reference and inspiration for further promoting the development of this field.
  • LUO Rongtao, LIU Xiaogang, TU Rongkun, JIANG Xiaofeng, XIE Zhengze, WEN Yue, GAO Feng, SHI Yuanjun, SHEN Caihong
    China Brewing. 2025, 44(12): 34-42. https://doi.org/10.11882/j.issn.0254-5071.2025.12.006
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    In order to explore the succession pattern and driving factors of microbial community structure during the third round of fermented grains during fermentation process of sauce-flavor (Jiangxiangxing) Baijiu, the changes of physicochemical factors and microbial community structure during fermentation process of fermented grains were analyzed by conventional analysis methods and high-throughput sequencing technology. The results were analyzed by cluster analysis (CA), linear discriminant analysis effect size (LEfSe), linear discriminant analysis (LDA), redundancy analysis (RDA) and co-occurrence network analysis. The results showed that 25 microorganism genera including Lactobacillus, Kroppenstedtia, Kazachstania, Thermoascus and others were the dominant genera (average relative abundance>1%). According to the succession pattern of microbial community and cluster analysis, the fermentation time nodes of fermented grains in the pit were divided into the early stage of fermentation (0-8 d) and the middle and late stage of fermentation (15-30 d). In the early stage of fermentation, Kroppenstedtia (LDA value>5) and Byssochlamys (LDA value>4) were the bacterial marker species and fungal marker species with the highest LDA value, respectively. In the middle and late stage of fermentation, Lactobacillus (LDA value>5) was the bacterial marker species with the highest LDA value. Correlation analysis results showed that starch and reducing sugar contents were the driving factors of bacterial community succession in the early stage of fermentation, which were significantly positively correlated with Kroppenstedtia, Thermomyces and other microorganisms (P<0.05). Moisture content and acidity were the driving factors of bacterial community succession in the middle and late stages of fermentation, which were highly positively correlated with Lactobacillus (P<0.001). Compared with fungal community, physicochemical factors had a greater impact on bacterial community. The results of co-occurrence network analysis results showed that 85 pairs of dominant genera were significantly positively correlated and 18 pairs were significantly negatively correlated (P<0.05), and the correlation between bacterial genera was stronger than that of fungal genera.
  • YANG Qingqing, ZHAO Wei, CUI Meilin, WANG Jiali, ZHANG Xiuhong
    China Brewing. 2025, 44(12): 43-50. https://doi.org/10.11882/j.issn.0254-5071.2025.12.007
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    Using Lactiplantibacillus plantarum Y36 isolated from light-flavor (Qingxiangxing) Baijiu fermented grains as research object, its biological characteristics and the inhibition of its fermentation supernatant on closely related strains from the same fermented grains were explored by turbidimetric method and the plate counting method, and the influences of fermentation conditions and exogenous additives on the inhibitory effect were investigated. The results indicated that in terms of growth characteristics, L. plantarum Y36 exhibited a S-shaped growth curve, entering the logarithmic phase at 4 h and reaching the stationary phase at 18 h. The pH of the fermentation broth rapidly decreased from 5.50 to 4.09 within 0-12 h. The optimal fermentation temperature and initial pH were 37 ℃ and 6.0, respectively. In terms of tolerance, the strain Y36 could survive under the environmental conditions of pH 3.0, bile salt content 0.3% and ethanol volume fraction 6%. After 7 h of artificial simulated gastrointestinal treatment, the survival rate of strain Y36 decreased to 2.2%. The research results on inhibition of closely related strains from the same fermented grains showed that the fermentation supernatant of strain Y36 had varying degrees of inhibitory effects on lactic acid bacteria from the same source. Among them, the inhibition rate on Lactobacillus brevis MC46 was the highest (61.25%). Under the conditions of fermentation time 24 h, temperature 37 ℃ and initial pH 6.0, the inhibition rates of strain Y36 fermentation supernatant on strain MC46 were 73.70%, 42.19% and 33.71%, respectively. With the addition of glycerol, Tween-80, ethanol and NaCl of 3%, 0.5%, 2.5%, and 0.5%, the inhibition rates of strain Y36 fermentation supernatant on strain MC46 were 66.98%, 72.16%, 68.35% and 48.31%, respectively.
  • LV Jiaxuan, TAO Yujie, WANG Rong, ZHU Derui, XING Jiangwa
    China Brewing. 2025, 44(12): 51-58. https://doi.org/10.11882/j.issn.0254-5071.2025.12.008
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    The encoding gene of the salt-tolerant carboxylesterase Est40 from the halophilic bacterium Vreelandella sp. CH40 was obtained through artificial synthesis technology. After introducing restriction enzyme cutting sites and tag sequences via polymerase chain reaction (PCR), the gene was integrated into the expression vector pET-22b(+) to obtain recombinant plasmid, and the recombinant plasmid was transformed into Escherichia coli Rosetta (DE3) host cells to construct a high-yield salt-tolerant carboxylesterase Est40 recombinant strain. Further, using recombinant carboxylesterase Est40 activity as the response value, the fermentation conditions for the recombinant strain were optimized using single-factor, Plackett-Burman and Box-Behnken response surface experiments. The results showed that the recombinant carboxylesterase Est40 was efficiently and solubly expressed in the E. coli expression system. The optimal fermentation conditions for the recombinant strain producing carboxylesterase Est40 were as follows: sucrose 35 g/L, MgSO4 12 g/L, yeast extract 15 g/L, NaCl 20 g/L, initial pH 7.5, induction temperature 24 ℃, and inoculum 6%. Under these optimal conditions, the activity of recombinant carboxylesterase Est40 reached (378.63±2.76) U/ml, which was 38.61% higher than that before optimization [(273.17±2.27) U/ml]. The research results provided important process parameters and theoretical basis for the subsequent industrial-scale production.
  • ZHOU Zhan, GAN Jinming, LIAO Bei, WANG Hongbiao, FANG Shangling, LI Hui, CHEN Maobin
    China Brewing. 2025, 44(12): 59-66. https://doi.org/10.11882/j.issn.0254-5071.2025.12.009
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    To analyze the differences in the composition of microbial community structure of rice wine koji in Vietnam, taking rice wine koji samples (YB, Y2G, YNNB, YNG1, YNG2) from five regions in Vietnam as the research objects, the physicochemical indicators were detected by conventional methods, the microbial community structure was analyzed by MiSeq high-throughput sequencing technology, and principal coordinate analysis (PCoA) and correlation analysis were conducted based on the results. The results showed that there were certain differences in saccharification power and liquefaction power among different koji samples. The saccharification power of sample YNNB and the liquefaction power of sample YNG1 were the highest, which were 306.63 mg/(g·h) and 1.02 g/(g·h), respectively. There were significant differences in microbial flora of different rice wine koji samples. The richness and diversity of bacteria and fungi in sample YNG1 were the highest. A total of 10 dominant bacterial genera and 13 dominant fungal genera (relative abundance>1%) were detected in 5 samples. The common dominant bacterial genus was Lactobacillus , Candida was the common dominant fungal genera in samples YNNB and Y2G, and Aspergillus was the dominant fungal genera in the samples YNG1, YNG2 and YB. PCoA could effectively distinguish rice wine koji samples in different regions. Analysis results of the microbial correlation network indicated that the nodes among bacterial genera were closely connected, while the connectivity among fungal genera was relatively low. Pantoea was significantly positively correlated with liquefaction power (P<0.05), and Acetobacter was significantly negatively correlated with liquefaction power (P<0.05).
  • ZHU Junying, GAO Wei, ZHOU Mengjie, YUAN Meng, YU Dengyang, CHEN Maobin, LI Liang, DONG Xiaoyuan, YANG Yong, HE Ping, ZHANG Yu
    China Brewing. 2025, 44(12): 67-73. https://doi.org/10.11882/j.issn.0254-5071.2025.12.010
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    In order to analyze the fermentation mechanism in Dacha and Ercha fermented grains of light-flavor (Qingxiangxing) Baijiu, the physicochemical indexes and microbial community in Dacha and Ercha fermented grains during fermentation process were analyzed by conventional physicochemical detection methods combined with high-throughput sequencing technology, and principal coordinate analysis (PCoA) and redundancy analysis (RDA) were conducted based on the detection results. The results showed that the change trends of moisture, alcohol content and starch content were similar in Dacha and Ercha fermented grains during the fermentation process, while there were certain differences in the change trends of acidity and reducing sugar content. At the end of fermentation, compared with the Ercha fermented grains, the alcohol content, moisture, reducing sugar, starch content and acidity of Dacha fermented grains were higher, which were 6.98%vol, 63.23%, 5.12%, 14.22% and 2.97 mmol/10 g, respectively. A total of 3 dominant bacterial phyla, 2 dominant fungal phyla, 5 dominant bacterial genera and 7 dominant fungal genera (relative abundance>1%) were detected during the fermentation process of Dacha and Ercha fermented grains. The PCoA results indicated that in the early stage of fermentation (0-7 d), the bacterial community structure of Dacha and Ercha fermented grains was relatively similar, while the fungal community structure was quite different. RDA analysis indicated that starch was significantly positively correlated with Saccharomycopsis (P<0.05) and extremely significantly negatively correlated with Lactobacillus (P<0.01). As a key driving factor for the succession of bacterial and fungal community structure, its consumption directly drived the alternation of bacterial and fungal communities.
  • WEI Yang, WANG Songtao, YANG Yang, TU Rongkun, ZHANG Liqiang, RAN Maofang, SUN Li, LI Shu, DENG Bo, SHEN Caihong, ZHAO Qiuwei
    China Brewing. 2025, 44(12): 74-81. https://doi.org/10.11882/j.issn.0254-5071.2025.12.011
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    The effect of early turning (ET), normal turning (NT), and delayed turning (DT) on the quality of high-temperature Daqu was systematically analyzed. The results indicated that prolonging the turning lowered both the cooling point and the secondary temperature-rise point of Qupi. The moisture, acidity, reducing sugar and starch content of high-temperature Daqu were affected by Daqu turning time. The early turning group exhibited the highest acidity [(0.2-2.6) mmol/10 g] and reducing sugar content [(0.8-4.1) g/100 g]. The content of aldehydes such as furfuryl alcohol, phenylethanol, trimethylpyrazine, and furfural in the yeast sample was relatively high, and the characteristics of baking and sauce aroma were more prominent. Furthermore, based on variable importance in the projection (VIP) value>1.5, 11 substances including isovaleric acid, furfural, and trimethylpyrazine were screened as key differential flavor compounds for Daqu. The early turning group had the highest saccharification power (149 mg/g·h), the delayed turning group showed the highest fermentation power [0.22 g/(0.5g·72 h)], and the normal turning group demonstrated the highest esterification power [127 mg/(50 g·7 d)]. The proportion of white Daqu in the early turning group was the lowest (5.68%). The Mantel test results showed that acidity, Qupi temperature, and alcohols content had a extremely significant effect on the quality of Daqu (P<0.01). The Pearson correlation analysis results showed that there was a highly significant positive correlation between the reducing sugar content and the temperature, alcohol content of Qupi (P<0.001). There was a highly significant positive correlation between the Qupi temperature and the room relative moisture, as well as the content of alcohols, aldehydes, and phenols (P<0.001). This study demonstrated that turning time was a critical control point for regulating the physicochemical properties and flavor profile of Daqu, and the early turning was more effective during summer production.
  • ZHAO Jinsong, PENG Jingjing, FANG Yuli, LIU Mingming, BIAN Minghong
    China Brewing. 2025, 44(12): 82-89. https://doi.org/10.11882/j.issn.0254-5071.2025.12.012
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    Activated carbon, owing to its highly developed pore structure, is widely employed in the purification and aging acceleration of Baijiu. However, existing research predominantly focuses on process optimisation for activated carbon models, and there is a lack of systematic analysis of influence of how pore structure parameters on Baijiu quality during adsorption process. In this study, using 11 types of activated carbon for liquor (1#-11#) and different quality of strong-flavor (Nongxiangxing) Baijiu as research subjects, a quality evaluation model was constructed, and the effect of activated carbon pore size on Baijiu quality and the removal patterns of off-flavors were analyzed. The results demonstrated that the actual quality grade of Baijiu was completely consistent with the model (100% concordance) by analytic hierarchy process. After treatment with the 11 activated carbon, the off-flavors such as bitterness, astringency, and foul odours were markedly reduced, the total acid, total ester and volatile flavor components contents in Baijiu all decreased significantly (P<0.05), while the solid content showed no significant change (P>0.05). In addition, the sensory score was significantly improved (P<0.05). Further analysis revealed that mesoporous activated carbon demonstrated superior efficacy in enhancing Baijiu quality compared to microporous activated carbon. These findings provided scientific theory and technical support for the improvement of the quality level of strong-flavor Baijiu by activated carbon for alcohol.
  • FAN Xiaoning, YIN Juyi, LIN Yixin, MA Wenchao, YAN Wei, TANG Yun*
    China Brewing. 2025, 44(12): 90-94. https://doi.org/10.11882/j.issn.0254-5071.2025.12.013
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    The differences in bacterial community structure between artificially aged 1-year pit mud (Apm) and naturally aged 10-years and 50 years pit mud (Ypm, Opm) were analyzed by Illumina MiSeq high-throughput sequencing technology, and the metabolic functions of bacterial communities were predicted and analyzed using PICRUSt2 software. The results showed that the richness and diversity of bacterial communities were the highest in the Opm sample, followed by the Ypm sample, and the lowest in the Apm sample. A total of 38 bacterial phyla and 488 bacterial genera were annotated from all pit mud samples. The main differences in the bacterial community structure between Apm sample, and Ypm and Opm samples were reflected in the relative abundance. At the phylum level, the relative abundance of Proteobacteria in the Apm sample was the highest (77.26%), while in the Ypm and Opm samples, the relative abundance of Firmicutes were the highest, at 70.99% and 80.60%, respectively. At the genus level, the relative abundance of Anaerosalibacter in the Apm sample was the highest (17.56%). Caproiciproducens, as a key bacterial genus for evaluating the maturity of cellar mud, had the highest relative abundance in the Ypm and Opm samples, which were 10.44% and 39.91%, respectively. The relative abundance of Caproiciproducens in the Apm sample was the lowest (2.98%). The bacterial community of pit mud mainly involved 12 major metabolic pathways, among which the carbon source metabolism, amino acid metabolism, energy metabolism, cofactor and vitamin metabolism, and lipid metabolism had the strongest ability. The metabolic strength of pit mud bacterial community was ranked as Opm>Ypm>Apm.
  • LONG Yafei, RAO Wei, TANG Lin, ZHONG Yuan, DUAN Qijun, LONG Shenggang, WU Baolin, ZHANG Qihang, LI Lihong*
    China Brewing. 2025, 44(12): 95-101. https://doi.org/10.11882/j.issn.0254-5071.2025.12.014
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    In order to explore the formation rules of pyrazines in fermented grains during the stacking fermentation of sauce-flavor (Jiangxiangxing) Baijiu, the changes in pyrazines, microbial communities and physicochemical indicators in fermented grains during the stacking fermentation of sauce- flavor Baijiu were respectively analyzed by headspace solid-phase microextraction coupled with gas chromatography-tandem mass spectrometry (HS- SPME-GC-MS/MS), metagenomic sequencing technology, and conventional methods, and the correlation analysis was conducted.The results showed that a total of 35 pyrazines were detected in fermented grains during the pile fermentation, with the higher concentrations being 2-acetyl-3-methylpyrazine (3.83-5.48 μg/g) and tetramethylpyrazine (1.58-2.10 μg/g). During the pile fermentation process, reducing sugars, total acid, starch, and protease generally exhibited a decreasing trend, while crude protein, moisture, saccharifying enzyme, and α-amylase activity showed an overall increasing trend. The bacterial microbial community structure in the fermented grains remained basically unchanged after pile fermentation, whereas significant differences were observed in the fungal microbial community structure. Correlation analysis indicated that 2,5-dimethyl-3-(3-methylbutyl)-pyrazine and tetramethylpyrazine had a strong correlation with microorganisms and physicochemical indicators. 2,5-dimethyl-3-(3-methylbutyl) -pyrazine was extremely significantly correlated with genera such as Scopulibacillus and Millerozyma, as well as crude protein and glycase (P<0.01). Tetramethylpyrazine was extremely significantly correlated with Oceanobacillus, Saccharpolyspora and acidic protease (P<0.01). Therefore, the production of pyrazines was closely related to the microbial flora and physicochemical indicators.
  • WANG Xi, HE Ping, ZHAO Rongshou, GAO Zhanzheng, LUO Wei, MA Chunyan, ZHANG Dan
    China Brewing. 2025, 44(12): 102-108. https://doi.org/10.11882/j.issn.0254-5071.2025.12.015
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    In order to explore the difference of medium-high temperature Daqu with different processes, the fungal community structure of mechanical and artificial Daqu were analyzed by high-throughput sequencing, the physicochemical indexes were determined by normal methods, and the correlation among fungal communities and between them and physicochemical indexes was analyzed. The results showed that the moisture, acidity and core temperature of artificial Daqu were higher, while the saccharification and liquefaction power of mechanical Daqu were higher during the fermentation process. The dominant fungal genera (relative abundance≥1%) in the two types of Daqu were both Alternaria, Mrakia and Fusarium in the room- loading stage, while Thermomyces, Rhizomucor and Rhizopus in the room-unloading stage. During the fermentation period, the relative abundance of Thermomyces in the artificial Daqu was higher, while the relative abundance of the Rhizopus was higher in the mechanical Daqu. There were 15 fungal genera, including Dipodascus, Torulaspora, and Debaryomyces, that exhibited differences between mechanical and artificial Daqu. Certain correlations were found among fungal genus and between fungal genus and physicochemical indexes of Daqu. This study revealed the differences of fungal community structure and the correlation between fungal genera in medium-high temperature Daqu with different processes, and provided a theoretical reference for promoting the mechanized production of Daqu.
  • CHEN Xiaolong, ZHANG Tingfeng, WU Fengfeng, YANG Lin, KANG Jia, LI Caixia
    China Brewing. 2025, 44(12): 109-115. https://doi.org/10.11882/j.issn.0254-5071.2025.12.016
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    Taking pickles from Linxia region, Gansu Province as the material, lactic acid bacteria were isolated by the calcium dissolution ring method. Their species were identified by morphological observation, physiological and biochemical experiments, and molecular biology techniques, and their biological characteristics such as tolerance, enzyme production capacity, hemolysis, drug sensitivity and antibacterial property were analyzed. The results showed that a total of 7 strains of lactic acid bacteria (numbered as B2-3-1 to B2-3-4, and B2-3-6 to B2-3-8) were isolated. After identification, strain B2-3-1 was Weissella confusa, strains B2-3-2 and B2-3-3 were Leuconostoc lactis, strain B2-3-4 was Streptococcus thermophilus, strains B2-3-6 and B2-3-8 were Lactiplantibacillus plantarum, and strain B2-3-7 was Lactobacillus paracasei. Among them, strain B2-3-8 could grow at pH 2, strains B2-3-6 and B2-3-8 could grow at bile salt mass concentration 0.7 g/L, strains B2-3-1, B2-3-6, and B2-3-8 could grow at NaCl 12%, strains B2-3-3 and B2-3-6 could grow at 80 ℃ treatment, strain B2-3-7 possessed protease production capability (D/d=2.18), and the intracellular β-galactosidase activity of strain B2-3-8 was 36.72 U/ml. All 7 strains exhibited low susceptibility to the antibiotics chloramphenicol, gentamicin, and levofloxacin. Strains B2-3-3, B2-3-7 and B2-3-8 exhibited inhibitory activity against both Staphylococcus aureus and Escherichia coli. Strains B2-3-1, B2-3-4, B2-3-7 and B2-3-8 showed no hemolysis. In conclusion, strain B2-3-8 demonstrated excellent biological characteristics.
  • HUANG Qiuwei, LONG Lingyun, AI Jingwen, HUANG Xinxin, LIU Gongde, SU Yanlan, XIE Chaomin, CHEN Ganlin
    China Brewing. 2025, 44(12): 116-123. https://doi.org/10.11882/j.issn.0254-5071.2025.12.017
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    Using papaya pickles as the research object, the composition and succession of microbial communities during the low-temperature pickling fermentation (0, 5, 10 and 20 d) were analyzed by 16S rRNA high-throughput sequencing technology. The results showed that during the pickling period 0-20 d, the microbial richness and diversity of the pickled samples exhibited a trend of an initial decrease followed by an increase with the prolongation of pickling time. The microbial richness and diversity were the highest at the beginning of pickling, then reached the lowest at 10 d, and subsequently stabilized after pickling for 10 d. The distribution of microbial communities showed that the main dominate phyla (relative abundance>1%) was Firmicutes (28.12%- 99.11%), the main dominate genera (relative abundance>1%) was Weissella (21.47%-97.66%), and the main dominate species (relative abundance>1%) were Weissella confusa (1.88%-8.56%) and Weissella paramesenteroides (2.02%-37.08%). The results of the diversity analysis of microbial community composition among samples indicated that there were significant differences in microbial species among samples at different pickling fermentation stages. Specifically, comapred with the microbial community structures of samples not pickled or pickled for 5 d, the samples pickled for 10 d and 20 d was more similar. Correlation analysis results indicated that Weissella dominated the low-temperature pickling process. The low-temperature pickling process for papaya pickles was conducive to the stable growth of Weissella as the core genus in the entire pickling process.
  • TAN Qibo, ZHANG Jie, YANG Ming, JIA Jianhua, GAN Linyao, SUN Lin, GU Kaiqin, FAN Kuanxiu, CHEN Xiangmei
    China Brewing. 2025, 44(12): 124-135. https://doi.org/10.11882/j.issn.0254-5071.2025.12.018
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    Taking high-temperature Daqu in Jinsha as the research object, the flavor wheel of high temperature Daqu was drew, and the characteristic flavor of high temperature Daqu was analyzed by headspace-solid-phase microextraction-gas chromatography-tandem mass spectrometry (HS-SPME- GC-MS/MS) and gas chromatography-olfactory (GC-O) technology. The results showed that a flavor wheel of high-temperature Daqu in Jinsha composed of 45 sensory descriptive words was established. The results of sensory quantitative description analysis (QDA) showed that the aroma of high- temperature Daqu in Jinsha mainly consisted of sauce aroma, grain aroma, floral and fruity aroma, burnt aroma, baking aroma and sweet aroma. A total of 195 volatile substances were detected in the high-temperature Daqu in Jinsha by GC-MS/MS, among them, there were 48 key volatile flavor substances with odor activity value (OAV) >1, and 66 volatile flavor substances were screened out in combination with GC-O analysis. A total of 19 characteristic volatile aroma compounds in high temperature Daqu in Jinsha such as isobutyral and 2-methylpyrazine, with OAV>1 and detected by GC-O, were screened out. Through Spearman correlation analysis, it was concluded that in the high temperature Daqu in Jinsha, the sauce aroma, grain aroma and floral and fruity aroma were highly significantly positively correlated with 7 compounds such as ethyl 2-methylbutyrate and isopentanol (P<0.001). The burnt aroma, baking aroma and sweet aroma were highly significantly positively correlated with 6 compounds such as 2-acetylpyridine and hexanal (P<0.001).
  • CAO Huajie, GUO Xinhao, AN Zhicheng, GAO Xingjia, YANG Jun, CHEN Ning, FAN Xiaoguang
    China Brewing. 2025, 44(12): 136-143. https://doi.org/10.11882/j.issn.0254-5071.2025.12.019
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    To enhance the adenosine synthesis capability of Bacillus subtilis, using an adenosine-producing strain B. subtilis XGL as the starting strain and mutagenesis treatment was carried out using atmospheric and room temperature plasma (ARTP), high-yield adenosine strains were screened through deep-well plate cultivation and rapid analysis and detection by microplate reader. The fermentation medium was optimized with Plackett-Burman tests, the steepest ascent tests, and response surface tests, and adenosine was efficiently produced by two-stage dissolved oxygen control strategy. The results showed that a high-yield adenosine strain XGL85 was screened by high-throughput mutagenesis, the adenosine yield produced by shake flask fermentation was 11.32 g/L, which was 39.5% higher than that of the starting strain XGL, and it had good genetic stability. The optimal fermentation medium for strain XGL85 were as follows: glucose 140 g/L, monosodium glutamate 12 g/L, yeast extract 18 g/L, sodium gluconate 1 g/L, (NH4)2SO4 12 g/L, MgSO4·7H2O 5 g/L, K2HPO4 2 g/L, CaCl2 1.3 g/L, and corn steep liquor 15 g/L. Under these optimal conditions, the yield of adenosine produced by shake flask fermentation of strain XGL85 was 17.51 g/L, which was 54.7% higher than that before optimization. When the strain XGL85 was subjected to two-stage dissolved oxygen controlled fermentation in a 30 L fermenter for 45 h, the adenosine yield reached 51.8 g/L and the glycoside conversion rate reached 20.4%.
  • LEI Jiayu, CONG Ning, DU Yonggang, ZHANG Yujiao, ZHANG Sufang*
    China Brewing. 2025, 44(12): 144-150. https://doi.org/10.11882/j.issn.0254-5071.2025.12.020
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    To investigate the application of low pectin methylesterase activity pectinase (WP) produced by Bacillus velezensis in the fermentation of mandarin wine, the differences in juice yield and clarity, the physicochemical properties, methanol content, total phenol and total flavonoid content, antioxidant activity, and sensory evaluation between the juice and wine of pectinase WP addition group (WP), non-pectinase addition group (NP), and the commercially available pectinase addition group (XS, NW) were compared. The results showed that WP treatment significantly reduced the methanol content in orange juice and fruit wine [(16.85±0.92) mg/L and (96.55±3.61) mg/L], with significant reduction of 53.00% and 33.13%, and 61.91% and 23.49%, respectively (P<0.05), compared to the XS and NP groups. Compared with the NP, XS and NW groups, the juice yield [(74.45±1.03)%] and clarity [(83.16±0.58)%] were significantly increased, and the fruit wine in the WP treatment group had higher total phenols [(1 465.01±16.10) mg GAE/L] and total flavonoids [(153.95±3.34) mg RE/L], as well as significantly higher DPPH [(287.25±3.15) mg GAE/L] and ABTS [(872.46±4.50) mg TE/L] free radical scavenging capacity. The sensory evaluation results showed that the taste, color and sweet and sour scores (3.83 point, 4.38 point, 3.89 point) of the WP group were better than those of the other groups. In conclusion, WP pectinase with low pectin methysterase activity could effectively reduce the methanol content in mandarin juice and wine, and improve the comprehensive quality of mandarin wine.
  • CHANG Songhan, ZHANG Wenhui, ZHANG Leqi, WANG Jiexing, FANG Yulin, ZHOU Guochao, JU Yanlun
    China Brewing. 2025, 44(12): 151-156. https://doi.org/10.11882/j.issn.0254-5071.2025.12.021
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    Using 'Cabernet Sauvignon' grape as raw material, 'Cabernet Sauvignon' rose wine was made by using different juice-making methods (pressed juice and free run juice), and the basic physicochemical indexes, bioactive compounds, and aroma components of 2 rose wine were analyzed, and sensory evaluation was conducted. The results showed that the total sugar and total acid contents (46.212 g/L, 4.731 g/L) of rose wine of pressed juice were significantly lower than those of the rose wine of free run juice (50.812 g/L, 5.869 g/L) (P<0.05), the tannin, anthocyanin and pH value (2.229 mg/L, 0.176 mg/L, 4.105) was significantly higher than that of the rose wine of free run juice (2.016 mg/L, 0.167 mg/L, 4.010). The monomeric anthocyanins in the rose wine of pressed juice were mainly acylated anthocyanins, while those in the rose wine of free run juice were mainly non- acylated anthocyanins. The total contents of free esters and alcohols in the rose wine of pressed juice (1 078.477 μg/L, 217.597 μg/L) were significantly higher than those in the rose wine of free run juice (757.951 μg/L, 195.691 μg/L) (P<0.05), while the total contents of free aldehydes in the rose wine of pressed juice (3.225 μg/L) were significantly lower than those in the rose wine of free run juice (8.079 μg/L) (P<0.05). The total free aroma content in rose wine of free run juice was 74.40% that of the rose wine of pressed juice. The total bound aroma content in the pressed rose wine was 81.10% of that in the free run rose wine. The overall sensory evaluation of the rose wine of pressed juice was lower than that of the rose wine of free run juice, with slightly higher acidity. In conclusion, the rose wine of pressed juice could improve the color stability and antioxidant capacity of Cabernet Sauvignon rose wine to some extent, but its overall sensory evaluation was inferior to that of the rose wine of free run juice.
  • HUANG Zhiguo, LUO Renchun, LI Yanzhong, DENG Jie, WEI Chunhui, REN Zhiqiang, XIE Jun
    China Brewing. 2025, 44(12): 157-162. https://doi.org/10.11882/j.issn.0254-5071.2025.12.022
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    To enhance the proportion of thermotolerant microbial flora, heat treatment was implemented to reduce the initial proportion of thermolabile bacteria prior to the cultivation of artificial pit mud. In this study, the artificial pit mud was subjected to water bath heat treatment at 80 ℃ for 5 min before fermentation, using the pit mud without heat treatment as the control group, the physicochemical properties, microbial counts, and acid contents of the pit mud from the two groups were compared. The differences in volatile flavor substances in Baijiu were analyzed by partial least squares-discriminant analysis (PLS-DA), and the key differential compounds were screened using variable importance in the projection (VIP) values. The results showed that in comparison with the control group, the pH value of the heat-treated artificial pit mud significantly increased by 11.63% (P<0.05), the content of ammonium nitrogen significantly increased by 20.29% (P<0.05), the content of available phosphorus significantly increased by 36.66% (P<0.05), while there were no significant differences in moisture and humus content (P>0.05). The count of aerobic bacteria in the heat-treated artificial pit mud decreased significantly, while the count of anaerobic bacteria increased significantly (P<0.05). The ratio of caproic acid to acetic acid in the heat-treated artificial pit mud exceeded 1, which met the premium pit mud standard of a specific distillery. Baijiu samples with different heat treatment conditions could be distinguished by PLS-DA, and a total of 15 key differential components in Baijiu under different treatments were screened out based on VIP value>1. Compared with the Baijiu from the control group, the content of ethyl caproate in the Baijiu from the heat treatment group increased by 50.21%, while the content of ethyl acetate decreased by 36.66%, thereby achieving the effect of increasing caproate and decreasing acetate. In conclusion, the quality of the artificial pit mud after heat treatment was superior to that of the control group.
  • MAO Hao, YOU Long, TU Rongkun, MA Xue, TAN Wei, YU Qi, LI Jiawei, JIA Junjie, ZHENG Lei
    China Brewing. 2025, 44(12): 163-170. https://doi.org/10.11882/j.issn.0254-5071.2025.12.023
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    To investigate the causes of cold turbidity in sauce-flavor (Jiangxiangxing) base liquor and the effect of different post-treatment methods (normal temperature filtration and frozen filtration) on the quality of base liquor, the effects of different filtration methods were evaluated by sensory evaluation and physicochemical indicators, the volatile flavor compounds of low-temperature precipitates in the wine were determined by headspace- solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS), the volatile flavor compounds of liquor after filtration was detected by GC, and the differential flavor substances were screened through the variable importance in the projection (VIP) values of orthogonal partial least squares discriminant analysis (OPLS-DA). The results showed that the volatile flavor components in the precipitates were mainly long-chain fatty acid esters and alkane compounds, with ethyl palmitate accounting for the highest content (130.62 μg/mg). Under room temperature, ultrafiltration and activated carbon filtration exhibited excellent performance, but the Jiangqu aroma and baking aroma were weakened, while the harshness and astringency were enhanced. In contrast, diatomite and diatomite microfiltration membrane filtration showed average performance in removing cold turbidity, but the sensory quality were well preserved. Under freezing filtration conditions, the filtered base liquor had no cold turbidity with each treatment, however, the sauce flavor was weakened and the astringency was enhanced. The OPLS-DA model could effectively distinguish base liquor samples treated by different filtration methods. The main reason for the differences in sensory and flavor among them was caused by the changes in 17 differential flavor compounds with VIP>1, such as ethyl palmitate and 2-methylbutanol. In conclusion, diatomite microfiltration membranes could replace conventional diatomite filtration, while other filtration methods showed different performances under different conditions.
  • YI Xiang, HUANG Tianqi, CHEN Keyu, WANG Li, SHI Peng, LUO Gaojian, TONG Guoqiang, YANG Qiang
    China Brewing. 2025, 44(12): 171-175. https://doi.org/10.11882/j.issn.0254-5071.2025.12.024
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    Using bamboo leaf flavone extract as raw material, single metal ions Al3+, Fe3+, Cu2+, Na+, K+, Ca2+ were added to react with bamboo leaf flavonoids in ethanol solution, respectively. The color parameter and stability of the metal ion bamboo leaf flavone composite alcohol solution under high temperature conditions were determined. Then the intensity of free radicals in bamboo leaf flavone solution and metal ion (such as Al3+, Fe3+) bamboo leaf flavone composite alcohol solution was detected and compared by electronic paramagnetic resonance spectroscopy. The results showed that Al3+ and Fe3+ could rapidly complex with bamboo leaf flavonoids and cause extremely significant color changes in the bamboo leaf flavonoid alcohol solution (P<0.01); Cu2+ only complexed with bamboo leaf flavonoids under high temperature conditions, resulting in significant color changes (P<0.05). Na+, K+, Ca2+ did not complex with bamboo leaf flavonoids and did not undergo color changes. Al3+ enhanced the electron distribution of bamboo leaf flavonoids and improved their ability to remove DPPH· and O2-·, but weakened their ability to remove OH·. Fe3+ simultaneously weakened its ability to scavenge DPPH·, O2-· and OH·, due to its oxidative activity and damage to the structure of bamboo leaf flavonoids. Therefore, it was determined that metal ions Al3+ and Fe3+ had different effect on the color difference and antioxidant activity of bamboo leaf flavonoids due to their different influencing mechanisms.
  • ZHU Guanghua, ZHAO Xiaoning, CHEN Jiawei, LUO Fei, XUE Churan, FU Xiaofang, WANG Yang, LING Han, GAO Chengjun, ZHANG Yinghui, WANG Wenqi, ZHENG Zimiao, WANG Yuchen, YANG Xuewei
    China Brewing. 2025, 44(12): 176-181. https://doi.org/10.11882/j.issn.0254-5071.2025.12.025
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    To understand the flavor differences of dry red wines with different grape varieties from Shacheng region of Hebei, using dry red wines of different grape varieties (Marselan, Syrah, Cabernet Sauvignon, Petit Verdot) as the research objects, the physicochemical indicators and volatile flavor components were determined respectively by conventional detection methods and headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) technology. The key volatile flavor substances were screened in combination with odor activity value (OAV), and their sensory qualities were analyzed. The results showed that the alcohol content of dry red wine with different grape varieties ranged from 14.63%vol to 15.92%vol (P>0.05). The Marselan dry red wine had the highest total sugar and total acid contents, which were 5.82 g/L and 7.53 g/L, respectively. The contents of volatile acid and dry extract in Syrah and Cabernet Sauvignon dry red wines were the highest (0.57 g/L and 31.58 g/L, respectively). A total of 21 key volatile flavor substances (OAV>1) were detected, which were 16, 21, 16 and 19 in Marselan, Syrah, Cabernet Sauvignon and Petit Verdot dry red wines, respectively. Among them, there were 11 common key volatile flavor substances including ethyl hexanoate, geranyl acetone, etc. There was no significant difference in the sensory scores among the four different grape varieties of dry red wine (P>0.05), but the aroma characteristics were different.
  • PAN Cuiping, WU Zhonghong, YANG Yuhan, HE Zhe, LIU Gege, WANG Junjie, LI Yanlin, LI Huajia, LI Ke
    China Brewing. 2025, 44(12): 182-188. https://doi.org/10.11882/j.issn.0254-5071.2025.12.026
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    To investigate the effect of Monascus on the flavor and quality of plum wine, using Cuihongli as raw materials, plum wine without Monascus rice addition (SC) as control group, and plum wine fermented by Monascus rice and Saccharomyces cerevisiae (HS) as experimental group, the physicochemical indexes, color parameters and volatile flavor substances were analyzed by conventional detection method and headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS). Based on the detection results, orthogonal partial least squares-discriminant analysis (OPLS-DA) was conducted, and the sensory quality was analyzed by sensory evaluation. The results showed that compared with the control group, the pH value, a* value, alcohol content, and dry extract content of the plum wine fermented with Monascus rice increased significantly, which were 3.41, 12.42, 11.36%vol, and 28.70 g/L, respectively. A total of 30 and 35 kinds of volatile flavor substances were detected in the SC group and HS group samples, respectively. There were 28 common volatile flavor substances, 2 and 7 unique volatile flavor substances, respectively. Combining the variance analysis P<0.05, and variable importance in projection (VIP) value>1.0, a total of 23 volatile flavor compounds with significant difference were identified. The sensory score of plum wine in the HS group (54 points) was higher than that in the SC group (44.3 points). In conclusion, the mixed fermentation with Monascus rice was more suitable for the brewing of plum wine, which was conducive to enhancing the complexity, stability, and harmony of the aroma.
  • ZHANG Xin, REN Zhiqiu, YAN Chuangshuo, SUN Ying, WANG Yufan, LI Hongjun
    China Brewing. 2025, 44(12): 189-194. https://doi.org/10.11882/j.issn.0254-5071.2025.12.027
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    To explore the effect of enzymatic extrusion sorghum grain adjuvant on the quality of wort and beer, using the wort and beer prepared using sorghum grain adjuvant without extrusion as the control group, the composition and content of fermentable sugars, nitrogen-containing compounds, free amino acids in wort, and the volatile flavor substances in beer were analyzed by HPLC, amino acid analyzer, and GC-MS. The results showed that the contents of fermentable sugars and free amino acids in the wort of enzymatic extrusion sorghum grain adjuvant were 13.46 g/100 ml and 1.24 g/L, compared with the control group, which increased by 16.44% and 6.92%, respectively, and the total nitrogen and nitrogen-containing compounds with low molecular mass contents increased by 7.08% and 9.40%, respectively. The basic indicators such as foam holding capacity, diacetyl content, and alcohol content of beer made from enzymatic extrusion sorghum grain adjuvant met the requirements of GB/T 4928—2008 "beer", and better than those of the control group. The contents of volatile flavor substances in the beer increased from 21 in the control group to 24 in the experimental group. In particular, the four flavor substances were detected in the beer, including 2-methylbutanol, ethyl butyrate, citronellyl acetate, and ethyl 9-decenoate, which gave the beer a prominent fruity and rose aroma. The beer exhibited a coordinated flavor and taste, and therefore increasing the sensory score from 75 (control group) to 83. In conclusion, enzymatic extrusion sorghum grain as an adjuvant could increase the contents of fermentable sugars and free amino acids in wort, and further improved the physicochemical characteristics, flavor substances contents, sensory scores, and the overall quality was superior to the control group.
  • FAN Jie, WANG Yan, HAN Huijuan, YAN Hu, DAI Jiajun, CHANG Xuedong, ZOU Jing
    China Brewing. 2025, 44(12): 195-202. https://doi.org/10.11882/j.issn.0254-5071.2025.12.028
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    The quality differences of low-alcohol hawthorn wine brewed by different strains of Kluyveromyces marxianus and Angel wine-fruit wine- specific yeast were compared using fresh hawthorn as raw material. The preparation process of low-pectin hawthorn juice was optimized through single- factor experiments combined with response surface methodology. Using Angel wine-fruit wine-specific yeast as the control, four strains of K. marxianus (numbered as 1572, 1953, 30125, and 33373) were selected to brew low-alcohol hawthorn wine, and the effect of different strains on the quality of the wine was evaluated. The results showed that the optimal preparation conditions for low-pectin hawthorn juice were as follows: extraction temperature 87 ℃, time 17 min, and volume ratio of hawthorn pulp and edible alcohol 1.0∶2.7. Under these optimal preparation conditions, the pectin content of the hawthorn juice was 0.005 g/kg. No methanol was detected in the low-alcohol hawthorn wines fermented by the four strains of K. marxianus. The alcohol content, total acid and total sugar contents were 2.63%vol-2.73%vol, 13.10-13.48 g/L and 2.05-2.15 g/L, respectively. The chroma, hue, L* value, a* value and b* value of the hawthorn wine brewed by strain 1572 were 0.148, 1.94, 75.14, 28.77 and 15.34, respectively. The wine had deep red color. The sensory score, total ester, total phenol and total flavonoid contents were 7.70 points, 2.12 g/L, 5.11 g gallic acid equivalent (GAE)/L and 9.06 g rutin equivalent (RE)/L, respectively. In summary, K. marxianus strain 1572 had the potential for preparation of low-alcohol hawthorn wine.
  • YANG Gang, LIU Fenghao, GAO Xingyu, ZHOU Liwei, DING Yuanfei, FU Ying, LI Shujing, LV Huiwei
    China Brewing. 2025, 44(12): 203-209. https://doi.org/10.11882/j.issn.0254-5071.2025.12.029
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    Using barley malt, caramel malt, wheat malt, dandelion powder and hops as raw materials, a dandelion craft beer with xanthine oxidase (XOD) inhibitory activity and rich in a strong ester aroma was fermented by top-fermenting dry yeast. Taking the total ester content as the main evaluation index and the bitterness value as the reference index, the dandelion addition, initial pH, and fermentation temperature were optimized by single factor tests and the Box-Behnken response surface tests. The physicochemical and microbiological indicators, flavor substances and biological activities of the finished dandelion craft beer were analyzed. The results showed that the optimal brewing process of dandelion craft beer was as follows: dandelion addition 2.4 g/L, initial pH 4.8, and fermentation temperature 18 ℃. Under these optimal process conditions, the total ester content of dandelion craft beer was 27.648 mg/L, the total flavonoid content was 123.56 mg/L, the total higher alcohol content was 100.732 mg/L, the XOD inhibition rate was 20.81%, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate was 88.77%, and the sensory score was 93.33 points. Dandelion craft beer had in vitro antioxidant activity and XOD inhibitory activity, and its physicochemical and microbial indicators all met the requirements of relevant national standards.
  • FANG Fang, LIU Mingxin, YE Rui, YU Dan, YU Jianxing, ZHANG Yang, XU Tao, LI Jiayou
    China Brewing. 2025, 44(12): 210-217. https://doi.org/10.11882/j.issn.0254-5071.2025.12.030
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    In order to improve the flavonoids extraction rate from Hovenia dulcis, the H. dulcis was fermented using Rhizopus oryzae 1006, and the fermentation condition was optimized by single factor and response surface methodology, the stress resistance of H. dulcis flavonoids on Saccharomyces cerevisiae and the antioxidant activity of Huangjiu. The results indicated that through single-factor experiments and response surface experiments optimization, the optimal fermentation conditions were spore suspension 0.04 ml/g, glucose 0.04 g/g, ammonium sulfate 0.002 g/g, liquid-solid ratio 0.9∶1 (ml∶g), fermentation temperature 28 ℃ and time 24 h. Under the optimized condition, the flavonoids yield from H. dulcis was 6.44 mg/g, which was 36.17% higher than that of the unfermented H. dulcis. Compared with the control, the addition of flavonoids from H. dulcis could effectively improve the ethanol and high temperature resistance of S. cerevisiae. The addition of H. dulcis in Huangjiu also improved the DPPH, ABTS scavenging rate and total reducing ability. The non-targeted omics analysis results showed that a total of 148 flavonoids were detected, with 77 significantly differential metabolites (including 16 down-regulated and 61 up-regulated metabolites). In summary, the fermentation of H. dulcis by R. oryzae 1006 could increase the types and quantities of flavonoids, enhance their biological activity, and demonstrate promising application prospect.
  • PU Xilei, FANG Dandan, MA Yigang, SI Xupeng, FANG Guiping, PEI Longying
    China Brewing. 2025, 44(12): 218-224. https://doi.org/10.11882/j.issn.0254-5071.2025.12.031
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    The small white apricot wine was prepared using Xinjiang small white apricot as the raw material. The dynamic changes of physicochemical indicators during the fermentation process of the small white apricot wine were monitored and a correlation analysis was conducted. Nonlinear fitting of its fermentation kinetics was carried out using the Logistic model, the SGompertz model, the DoseResp model and the Boltzmann model. The results showed that the alcohol content of the small white apricot wine was 11.7%vol, the viable yeast count was 10.38×107 CFU/ml, the total sugar content was 3.98 g/L, the pH was 3.89, and the titratable acid content was 8.5 g/L. The fruit wine mainly contained quinic acid, malic acid, and citric acid, with contents of 1.29 g/L, 2.95 g/L, and 4.13 g/L, respectively. The correlation analysis showed that the viable yeast count was extremely significantly negatively correlated with total sugar and malic acid (P<0.01), total sugar was highly extremely significantly negatively correlated with alcohol content (P<0.001) and extremely significantly positively correlated with malic acid (P<0.01), alcohol content was extremely significantly positively correlated with tartaric acid (P<0.01). The fermentation kinetics analysis indicated that the optimal models for fitting the changes in viable yeast count, total sugar content, and alcohol content were the DoseResp model, Boltzmann model, and Logistic model, respectively. The correlation coefficients R2 were all >0.99, and the error between the predicted values and the experimental values was≤25%, indicating that the models could scientifically and accurately simulate the changes of key indicators during the fermentation process of the small white apricot wine. The experimental results could provide certain data support for the brewing of small white apricot wine.
  • LIU Fangchen, XU Mingqiang, LAN Sisi, QIU Bolin, LI Yuedong, CHEN Kai
    China Brewing. 2025, 44(12): 225-232. https://doi.org/10.11882/j.issn.0254-5071.2025.12.032
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    To investigate the quality changes during the processing of jujube cheese, the jujube cheese was prepared by solid-state fermentation using gray jujubes as raw materials and a mixture of fruit wine yeast and compound lactic acid bacteria (2∶1) as starter. The physicochemical properties, sensory score, texture, color parameters, and volatile flavor compounds were determined during the processing of jujube cheese. The results indicated that the total sugar content, sugar-to-acid ratio, total flavonoids content, and hardness exhibited a decreasing trend, while the sensory score, total phenol content, water activity, chewiness, elasticity, color difference, and browning degree showed an increasing trend during the fermentation process. The total acid content initially increased and then decreased. After sterilization and drying, the total sugar content stabilized at 12.89%, while the total acid, sugar-to-acid ratio, total phenols, total flavonoids, and water activity decreased to 0.65%, 19.98, 11.45 mg/g, 1.68 mg/g, and 0.897, respectively. In contrast, hardness, chewiness, elasticity, color difference, and browning degree increased to 708.144 N, 0.912, 149.108, 16.74, and 5.77, respectively. A total of 54 volatile flavor compounds were detected in the dried jujube cheese samples, including 20 esters, 9 alcohols, 7 aldehydes, 7 ketones, 2 alkanes, 1 alkene, and 8 other compounds. Among them, ester substances had the highest relative content, with the main compounds being ethyl acetate and ethyl propionate, which had rich wine aroma and strong fruity aroma.
  • SU Yue, WANG Wanxin, DUAN Cuicui, MA Fumin, LI Xiaolei, LI Dan*
    China Brewing. 2025, 44(12): 233-239. https://doi.org/10.11882/j.issn.0254-5071.2025.12.033
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    In this study, the physicochemical indicators, color intensity, sensory and volatile flavor substances of three brands of matsutake soy sauce (A, B, C) were determined by conventional physicochemical determination methods, gas chromatography ion mobility spectrometry (GC-IMS) method and electronic nose method. Differential analysis of volatile flavor substances was conducted using principal component analysis (PCA) and partial least squares analysis (OPLS-DA) methods. Differential volatile flavor substances were screened based on the variable importance projection (VIP) value >1. The results showed that there was no significant difference in the total acid content among the three types of matsutake soy sauce (P>0.05), while there were significant differences in the total sugar content and chromatic value (P<0.05). A total of 49 volatile flavor substances were detected by GC-IMS. The fingerprint chromatogram showed that the main volatile flavor substances shared by the three types of matsutake soy sauce were 2,3-pentanedione, n-butyral, tert-butanol, etc., which mainly provided the caramel flavor of the soy sauce. PCA and OPLS-DA could effectively distinguish three different brands of matsutake soy sauce. Based on VIP value >1, a total of 16 differentially volatile flavor substances were screened out. Among them, propylene glycol contributed sweetness, 2-methyltetrahydrofuran-3-one contributed baking aroma, and 2,6-dimethylpyriazine contributed umami taste. Sensory evaluation showed that the overall acceptability of brand A soy sauce was the optimal. To sum up, there were differences in the content of flavor substances and sensory characteristics among the three different brands of matsutake soy sauce.
  • LIU Yongfei, WU Maozhao, YAN Wenjun, WANG Bin, WANG Tao, REN Yanling, SHI Zibin, ZHANG Jianqiang
    China Brewing. 2025, 44(12): 240-247. https://doi.org/10.11882/j.issn.0254-5071.2025.12.034
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    The effect of secondary fermentation of Guizhou fermented peppers with the addition of sauce-flavor (Jiangxiangxing) Baijiu on flavor and quality were investigated. Physicochemical parameters and total phenolic content were compared between two fermentation modes: without Baijiu addition (KB group) and with Baijiu addition (JX group). Volatile flavor compounds were analyzed by headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS), and their odor activity values (OAVs) were calculated, and sensory evaluation was detected. The results showed that the addition of sauce-flavor Baijiu enhanced the acid-producing capacity of fermented peppers, significantly reduced the pH value (3.66) at 30 d of fermentation, while increasing total acidity (21.35 g/kg), amino acid nitrogen (0.099 g/100 g), and total phenolic content (4.45 mg/g). A total of 55 volatile aroma compounds with a concentration of (370.70±57.08) mg/kg were detected in the KB group, whereas 51 compounds with a higher total concentration of (473.57±101.68) mg/kg were identified in the JX group. The addition of Baijiu changed the composition of flavor substances, and OAV analysis indicated that eight compounds had higher OAVs in the JX group than in the KB group, with the OAV of ethyl propionate (233.70) was significantly higher than that in the KB group (7.78). Sensory evaluation results revealed that sauce-flavor Baijiu addition could improve the flavor deficiencies of fermented peppers and alleviate the problems of bitterness and musty odor. In conclusion, the fermented peppers in the secondary fermentation with sauce-flavor Baijiu addition showed greater flavor harmony, stronger aroma intensity, and superior quality.
  • DING Yan, ZHAI Mingyang, LIU Siyuan, TIAN Xiaoju, XI Qinqin, SU Shengyuan, YANG Jiayu
    China Brewing. 2025, 44(12): 248-256. https://doi.org/10.11882/j.issn.0254-5071.2025.12.035
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    In order to fully utilize the nutritional components of goji pomace, superior strain with better nutrional value enhancement of goji pomace were screened from 11 lactic acid bacterial strains. Using the antioxidant activity comprehensive score constructed based on the total phenol and total flavonoid content and ABTS+ free radical scavenging rate in the pomace as evaluation index, the solid-state fermentation process was optimized through single-factor experiments combined with response surface methodology. The results indicated that Lactiplantibacillus plantarum NXU-Q6 significantly improved the physicochemical properties of grape pomace. After 48 h of fermentation, the pH decreased to 4.25±0.03, total acid content increased to (14.93±0.02) mg/g, and total sugar consumption reached 62.06%. Soluble protein content increased to (0.53±0.01) mg/ml, while the cellulose mass fraction decreased to (6.00±0.11)%. After fermentation with strain NXU-Q6, the total phenolic and total flavonoid mass fractions increased by 65.92% and 70.89%, respectively. The ABTS+ radical scavenging rate also improved, reaching 94.67%. The results of response surface methodology showed that the optimal solid-state fermentation conditions were as follows: inoculum 7%, fermentation time 40 h, whey protein addition 14%, solid-liquid ratio 1∶1.1 (g∶ml), and fermentation temperature 32 ℃. Under these optimal conditions, the comprehensive score of antioxidant activity of goji pomace reached 0.996, and the total phenol, total flavonoid content, and ABTS+ free radical scavenging rate reached 39.17 mg/g, 31.34 mg/g, and 95.32%, respectively. The research results could achieve the reuse of goji pomace resources and had the potential to promote the green development of goji deep processing industry.
  • DENG Tujing, YUAN Lei, ZHONG Zhengchang, LAI Zhibin, SUO Langzhuoma, WANG Zhijian, LIU Yu
    China Brewing. 2025, 44(12): 257-264. https://doi.org/10.11882/j.issn.0254-5071.2025.12.036
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    To reduce the waste of apple pomace (AP), using apple pomace from Linzhi as raw material, apple pomace Jiaosu (APJS) was prepared by sequential fermentation with BV818 yeast and Lactobacillus pentosus. Taking the viable yeast and Lactobacillus pentosus counts, and superoxide dismutase (SOD) activity as evaluation indicators, the fermentation conditions for yeast and L. pentosus were optimized by response surface tests and orthogonal tests, and the physicochemical indexes and antioxidant capacity of apple pomace Jiaosu were analyzed. The results showed that the optimal fermentation conditions for yeast were as follows: sugar addition 5%, fermentation temperature 25 ℃, and yeast inoculum 0.20%. Under these conditions, the viable yeast counts and SOD activity reached 8.79 lg(CFU/ml) and 932.95 U/ml, respectively. The optimal fermentation conditions for L. pentosus were as follows: L. pentosus inoculum 1.5%, fermentation time 36 h and temperature 37 ℃. Under these conditions, the viable counts of Lactobacillus pentosus and SOD activity were 7.67 lg(CFU/ml) and 2 618.02 U/ml, respectively. The soluble solids, total sugar, reducing sugar, and pH of APJS were 7.23 °Bx, 16.25 mg/ml, 0.74 mg/ml and 3.61, respectively, which were significantly decreased compared with AP (P<0.05). The total acid, total flavonoids, γ-aminobutyric acid (GABA), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging rates, and ferric reducing/antioxidant power (FRAP) value of APJS were 48.19 mg/g, 0.46 mg/ml, 11.64 μg/ml, 60.57%, 78.12% and 1.36, respectively, all of which were significantly increased compared with AP (P<0.05).
  • NIE Long, MA Hailong, WANG Rong, LI Sijin, LUO Xuanfei, DONG Guangcun, ZHANG Qiuyan
    China Brewing. 2025, 44(12): 265-270. https://doi.org/10.11882/j.issn.0254-5071.2025.12.037
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    To overcome the limitations of traditional single-strain fermentation and conventional processing techniques, using Polygonatum kingianum as the raw material, Lactiplantibacillus plantarum Lp-29, Bifidobacterium animalis subsp. lactis WYB66 and Bifidobacterium longum WB195 as fermented strains, the P. kingianum Jiaosu was prepared, the fermentation process conditions were optimized through single-factor experiments and response surface methodology, and the bioactive component content and antioxidant capacity before and after fermentation were analyzed. The results showed that the optimal fermentation process conditions of P. kingianum Jiaosu were material-to-liquid ratio 1∶4 (g∶ml), inoculum 0.4%, brown sugar addition 11% and fermentation time 6 d. Under these optimized conditions, the comprehensive evaluation score of the P. kingianum Jiaosu reached 0.94 points. Compared with before fermentation, the total flavonoids, polysaccharides and total polyphenols contents after fermentation significantly increased from 0.462 mg/L, 23.94 mg/g and 0.68 mg/g to 0.996 mg/L, 37.21 mg/g and 2.36 mg/g, respectively (P<0.05), the pH decreased significantly from 4.15 to 3.31 (P<0.05), the scavenging rates of 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS+), and hydroxyl radicals significantly increased from 37.5%, 60.07% and 34.48% to 92.62%, 90.71% and 48.25%, respectively (P<0.05).
  • WANG Yifan, ZHANG Dawei, ZHANG Jie, WANG Yudong
    China Brewing. 2025, 44(12): 271-276. https://doi.org/10.11882/j.issn.0254-5071.2025.12.038
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    Using Robusta coffee beans as research object, with naturally fermented coffee beans as the control group and coffee beans fermented with different types of Jiuqu (YF, AN, JN, RH) as the experimental group, and cupping score as evaluation index, the fermentation process was optimized through single-factor and orthogonal tests, and the volatile flavor components were analyzed using headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS). The results indicated that YF Jiuqu was the optimal starter, the optimal fermentation conditions for the coffee beans were loading volume 60%, fermentation temperature 25 ℃, and time 36 h. Under these optimized conditions, the cupping score was 9.0 points. The results of HS-SPME-GC-MS showed that a total of 22 volatile flavor compounds with relative content difference>0.1% were detected inthe control group and the experimental group, including 5 phenols, 3 aldehydes, 3 pyrazines, 3 heterocyclics and 3 alkanes, 1 aromatic compound, 1 ester, 1 ketone, 1 alcohol and 1 ether. The relative content of aroma substances such as 5-vinyl-2-methoxyphenol and eugenol in coffee beans fermented with Jiuqu increased, while the aroma substances with a negative effect on the flavor (such as 2-ethylpyrazine with earthy flavor) decreased, which could effectively improve the flavor quality of coffee beans.
  • CHEN Kai, ZHOU Haiyan, WEI Wei, HUANG Lijuan, XIANG Yuanyuan, HE Yang, WEI Yutao, LI Daoxia
    China Brewing. 2025, 44(12): 277-285. https://doi.org/10.11882/j.issn.0254-5071.2025.12.039
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    A method for the simultaneous determination of 18 sweeteners in Baijiu was established with ultra-performance liquid chromatography-tandem mass spectrometry method, quantification was carried out using the matrix-matched standard curve external standard method under the dynamic multi- reaction monitoring mode (dMRM). The results showed that under the optimized conditions, the linear relationships of the 18 sweeteners were good within their respective mass concentration ranges, with correlation coefficients R2>0.999. The detection limit were 5.0-10.0 μg/kg, the quantification limit were 10.0-20.0 μg/kg, the recovery rates of low, medium and high levels in three flavor types of Baijiu were 61.1%-117.8%, and the relative standard deviation (RSD) were 0.4%-10.0%. The results of 18 sweeteners in 640 batches of Bajiu samples from different brands and flavor types showed that 15 sweeteners were detected in 125 batches of Bajiu samples. Among them, two or more sweeteners were simultaneously detected in 82 batches, accounting for 65.6% of the Bajiu samples detected with sweeteners samples. Among them, the top four in terms of detection rate were stevioside (0.02-48.5 mg/kg), rebodyoside (0.020-44.5 mg/kg), Dulcoside A (0.02-0.41 mg/kg), and cyclamate (0.02-0.49 mg/kg). This method featured high throughput, rapidity, high sensitivity, accuracy and reliability, and could be used for the simultaneous quantitative detection of 18 sweeteners in Baijiu.
  • GUAN Li, WU Xiaoyong, JIA Linfei, TAO Guisong, ZHANG Xin
    China Brewing. 2025, 44(12): 286-292. https://doi.org/10.11882/j.issn.0254-5071.2025.12.040
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    The UV-Vis absorption spectra of vinegar and vinegar adulterated in different proportions were collected using an UV-Vis spectrophotometer. The spectral data were preprocessed by Savitsky-Goyle (SG) smoothing and first-order derivatives. Feature extraction of high-dimensional spectra was carried out with the help of competitive adaptive reweighted sampling (CARS). A support vector machine (SVM) model was established using the extracted low-dimensional spectral data. The vinegar samples and adulterated vinegar samples with low-pressure carbonylation method glacial acetic acid were identified by applying this model, and the identification accuracy of SVM models constructed with different kernel functions was compared, with the aim of establishing a method for identifying adulterated low-pressure carbonylation method glacial acetic acid vinegar based on UV-Vis absorption spectra combined with pattern recognition. The results showed that the SVM models constructed using linear kernel functions and polynomial kernel functions had a 100% identification accuracy rate for vinegar and adulterated low-pressure carbonylation glacial acetic acid vinegar. The SVM models constructed using radial basis kernel functions and Sigmoid kernel functions could achieve an average identification accuracy rate of 90% for vinegar and adulterated vinegar with low-pressure carbonylation glacial acetic acid. Based on UV-Vis absorption spectroscopy combined with pattern recognition, an economical, efficient and accurate method for identifying glacial acetic acid adulterated in vinegar by low-pressure carbonylation was established, providing an innovative and feasible direction for the safety and quality supervision of vinegar.
  • MIN Yuhang, XIANG Qi, WU Ke, SHE Cheng, TANG Ruiyi, LIU Na, LI Jie, LAN Lan
    China Brewing. 2025, 44(12): 293-297. https://doi.org/10.11882/j.issn.0254-5071.2025.12.041
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    A rapid analytical method for the determination of γ-aminobutyric acid in beverages was established based on ultra-high performance liquid chromatogram-tandem mass spectrometry (UPLC-MS/MS), and the dietary risk of γ-aminobutyric acid in beverages was assessed. Sample was extracted with water and separated on Agilent Zorbax HILIC column (2.1 mm×100 mm, 3.5 μm) under the mobile phase system of acetonitrile-5 mmol/L ammonium acetate. The target compounds were scanned by electrospray ionization ion source in positive ion mode, detected in multiple reaction monitoring (MRM) mode, and quantified by external standard method. The potential dietary risk was evaluated by calculating the hazard quotient (HQ) value of γ-aminobutyric acid in beverages. The results showed that γ-aminobutyric acid showed good linearity in the mass concentration range of 1-200 ng/ml with the correlation coefficient greater than 0.999. The detection limit of the method was 25 μg/kg, and the limit of quantification was 50 μg/kg. The recovery rates were ranged from 99.08% to 101.21% at three different spiked levels of 50 μg/kg, 100 μg/kg and 500 μg/kg, and the relative deviations (RSDs) of precision tests results(n=6) were 1.12%-3.21%. The method established above was applied to determine 66 batches of various types of commercial beverage. The detection rate of γ-aminobutyric acid was 98.48%, and its content range was ND-314.0 mg/kg. The dietary risk assessment indicated that the hazard quotient (HQ) of γ-aminobutyric acid in these beverages was below the safety threshold of 1, indicating a low dietary exposure risk associated for such products.
  • QIE Guangping, CAO Yuhan, CHEN Shuizhen, WANG Min, HE Yubei, WANG Chenzheng, WANG Minzi
    China Brewing. 2025, 44(12): 298-302. https://doi.org/10.11882/j.issn.0254-5071.2025.12.042
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    The density distribution map of Baijiu production enterprises was drawn using the kernel density estimation (KDE) method by collecting the raster data of 19 bioclimatic variables and the geographical distribution data of Baijiu enterprises, then the correlation between it and bioclimatic variables was analyzed, and the industrial layout and resource allocation strategies for Guizhou were proposed. The results showed that the Baijiu production enterprises in Guizhou were mainly concentrated in Zunyi City, and the spatial kernel density values of the enterprises were highly significantly correlated with 19 bioclimatic variables and the digital elevation model (DEM) (P<0.001), among which there was a negative correlation with DEM, indicating that Baijiu enterprises were more distributed in low-altitude areas. It was negatively correlated with the diurnal range, isothermality, precipitation in the wettest month and precipitation in the warmest quarter, indicating that areas with smaller temperature differences and moderate and stable precipitation were more conducive to the stability of the fermentation environment for brewing microorganisms. It was positively correlated with the temperature seasonality, the highest temperature of the warmest month, the lowest temperature of the coldest month, the annual temperature difference, the lowest temperature of the wettest month and the average temperature of the warmest quarter. This indicated that Baijiu enterprises prefer to be distributed in areas with distinct cold and warm climates, high temperature and humidity, and not extremely cold winters. Such natural conditions were more conducive to the activation of fermentation power and the formation of the brewing micro-ecology. Therefore, in the future development of the Guizhou Baijiu industry, bio-climate variables should be incorporated into industrial planning, a climate adaptability evaluation system should be established, suitable development zones and restricted development zones should be scientifically demarcated, and the accuracy of site selection and resource allocation should be enhanced.
  • XIAO Rongyang, HU Tao, ZHAO Bingkun
    China Brewing. 2025, 44(12): 303-309. https://doi.org/10.11882/j.issn.0254-5071.2025.12.043
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    As a representative of "industry plus" model under the background of "cultural and tourism integration", Baijiu industrial tourism integrates industrial technology exhibition, cultural experience, leisure sightseeing, and other functions, demonstrating considerable development potential. However, its current development is confronted with challenges such as shallow experience level and content homogenization. In this study, taking the Luzhou Laojiao Intelligent Packaging Center as an example, its development potential as an industrial tourism resource was analyzed, the necessity for Baijiu industrial tourism to break through the traditional mode was emphasized, and its multidimensional value across economic, cultural, technological, and educational dimensions was deeply excavated. At same time, a series of development strategies and measures, including brand empowerment and targeted marketing, integration of technological interaction with science education, cultural excavation with experiential innovation, industrial extension with coordinated growth, and artistic enhancement with aesthetic value were proposed, aiming to promote it to become a vital channel for brand communication and value creation of Baijiu industrial tourism, and offer practical insights for the high-quality development of industrial tourism.
  • LIU Jingwei, YANG Ting, TONG Jun*
    China Brewing. 2025, 44(12): 310-315. https://doi.org/10.11882/j.issn.0254-5071.2025.12.044
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    Corporate competitiveness is the key factor for enterprises to obtain sustainable competitive advantage. Taking 18 Baijiu listed companies in 2023 as samples, and based on the main financial indicators, such as return on equity in their annual financial reports, using factor analysis (FA) cluster analysis (CA), the market competitiveness of Baijiu listed companies was comprehensively evaluated. Results showed that the competitiveness of listed Baijiu companies could be divided into three categories: good, average, and poor, with most companies at the average level. At the same time, in view of the profitability, development ability and operation capability, which reflected the competitiveness of Baijiu listed companies, the corresponding countermeasures and suggestions were put forward respectively.
  • ZHOU Jianhong, LI Huiyao
    China Brewing. 2025, 44(12): 316-320. https://doi.org/10.11882/j.issn.0254-5071.2025.12.045
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    Tourist satisfaction plays a vital role in advancing the development of liquor tourism. From the perspective of tourist satisfaction, the tourist comments on the online tourism platform related to Maotai Town were selected as the data source. The data source was analyzed and encoded at three levels using grounded theory, and combined with the three-factor theory, the influencing factors of tourist satisfaction in liquor tourism were explored. The results showed that the satisfaction of tourists in Maotai Town was mainly influenced by three factors: basic functions, embodied experience and cultural identity. Basic functions belonged to health care factors. Insufficient performance would significantly cause dissatisfaction. Embodied experience was a mixed factor. High performance enhanced satisfaction, while low performance led to dissatisfaction. Cultural identity was an incentive factor. Good performance could significantly enhance tourists' satisfaction. This indicated that, at the theoretical level, the satisfaction of tourists with liquor tourism had multi-dimensional and non-linear characteristics. At the practical level, tourist satisfaction should be enhanced from three aspects: basic functions, embodied experience and cultural identity.