This paper was to investigate the effects of SOD1 and SOD2 gene deletions on the yeast stress tolerance, including heat stress、ethanol toxicity、osmotic stress、salt stress、and nutrient starvation, through the spot dilution growth assays by using sod1Δ、sod2Δ and sod1Δ sod2Δ gene deletion mutants. The results showed that sod1Δ mutant exhibited decreased tolerance to heat stress、osmotic stress and acetic acid toxicity, sod2Δ showed no significant alteration of tolerance, sod1Δ sod2Δ double deletion mutants displayed lower tolerance to all stress conditions tested except nutrient starvation stress, as compared to wild type strain, which suggested that superoxide dismutase is essential for tolerance to various types of environmental stress in yeast.