In order to provide scientific grounds for industrialized production, the optimal inoculation times of seed liquid to solid media of Cordyceps militaris and C.takaomontana were investigated. The routine method was used to inoculate and counting the spores, taking mycelia dry weight, hyphal bodies and rate of mycelia coverage in solid media as indicators, The results showed that both Cordyceps species were fast to grow and vigorous during 3rd~6th day, which is the proper time to be inoculated, especially in the 4th day.