One-step ethanol production from Jerusalem artichoke can be carried out by using yeasts of Saccharomyces cerevisiae with inulinase activity.In this work,the influences of initial sugar and mineral salt concentration,temperature,seed culture medium and nitrogen source on ethanol fermentation of S.cerevisiae Y05 with higher inulinase activity were investigated by using Jerusalem artichoke juice as cultural media.The results showed that:(1) the optimal initial sugar concentration was 250g/L.Higher mineral salts in Jerusalem artichoke juice did not inhibit the ethanol fermentation;(2) the optimal temperature of ethanol fermentation with S.cerevisiae Y05 was 37℃;(3) iunlin as carbon source in seed culture medium was in favour of S.cerevisiae Y05 to produce more inulinase,which stimulate its ethanol fermentation;(4) its optimal inoculum size was 15%;(5) Jerusalem artichoke juice was a perfect cultural medium,but supplementation of nitrogen sources was necessary.Supplementation of 5.0g/L corn steep liquor largely enhanced initial sugar utilized,final ethanol concentration and ethanol yield of S.cerevisiae Y05.