Optimization of oil fermentation medium from inulin by Trichosporon cutaneum

JIA Shanshan;JIU Min;WANG Lunji

China Brewing ›› 2012, Vol. 31 ›› Issue (5) : 63-67.

PDF(369 KB)
PDF(369 KB)
China Brewing ›› 2012, Vol. 31 ›› Issue (5) : 63-67.

Optimization of oil fermentation medium from inulin by Trichosporon cutaneum

  • JIA Shanshan;JIU Min;WANG Lunji
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Abstract

The use of Plaekett-Burman design and response surface analysis method for yeast fermentation to produce oil,optimize the culture medium,the first Plaekett-Burman design of fermentation medium components of inulin,yeast extract,magnesium sulfate,ferrous sulfate,potassium dihydrogen phosphate,calcium chloride,zinc sulfate were evaluated and selected has the remarkable effect of yeast extract,zinc sulfate,calcium chloride,and then use the path of steepest ascent approximation of the maximum response value,then the use of Box-Behnken area,experimental design and response surface analysis method for regression analysis.The results showed that yeast extract,zinc sulfate,calcium chloride in oil content had the remarkable correlation.By solving the regression equation to get the optimization of fermentation medium components: yeast extract 6.68g/L,zinc sulfate 0.457g/L,calcium chloride 0.342g/L.On the optimization of medium for fermentation culture,its biomass and grease content reached 13.56g/L,5.281g/L,fat content increased by 11.2% than before optimization.

Key words

inulin / Trichosporon cutaneum / oil / response surface analysis

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JIA Shanshan;JIU Min;WANG Lunji. Optimization of oil fermentation medium from inulin by Trichosporon cutaneum[J]. China Brewing, 2012, 31(5): 63-67
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