The fermentation production condition optimization of nata-producing strain was studied in this paper.The results show that the nata-producing strain was obtained from broth,and the optimum medium of nata fermentation is as follows: glucose of 2%,peptone of 1%,yeast extract of 0.5%,citric acid of 0.1% and disodium hydrogen phosphate of 0.5%.The yield of nata-producing strain is 9.9g/L.