Using sauce-flavor Baijiu (Chinese liquor) distiller's grains as the research object, the melanoidin in the distillers' grains were extracted and determined, and the extraction conditions were optimized by single factor and orthogonal experiments. The results showed that the optimal process for melanoidin extraction by ethanol leaching method was extraction time 3 h, temperature 55 ℃, and solid-liquid ratio 1∶25 (g∶ml), under the optimal condition, the melanoidin extraction rate was 51.25%. The optimal ultrasonic auxiliary solution immersion method for melanoidin extraction was ultrasonic time 25 min, temperature 75 ℃, solid-liquid ratio 1∶37 (g∶ml), ethanol volume fraction 50%, and ultrasonic power 320 W. Under the optimized conditions, the extraction rate of melanoidin was 72.37%. The results showed that the ultrasonic auxiliary solution immersion method was superior to the ethanol solution leaching method.