Screening of Saccharomyces cerevisiae with high glutamic acid content and optimization of fermentation medium

LIU Licong1, HU Yiwen1, WANG Zhi1, DAI Jun1, LI Xin1, YAO Juan2, ZHENG Guobin2, LV Zhengbo3, CHEN Xiong1*

China Brewing ›› 2017, Vol. 36 ›› Issue (6) : 116-120.

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China Brewing ›› 2017, Vol. 36 ›› Issue (6) : 116-120. DOI: 10.11882/j.issn.0254-5071.2017.06.024

Screening of Saccharomyces cerevisiae with high glutamic acid content and optimization of fermentation medium

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Abstract

A Saccharomyces cerevisiae F-5 with high glutamic acid content was screened from home-made wine. The homology of its 26S rDNA sequence with the 26S rDNA sequence of S. cerevisiae TY12 was 100%. Using the intracellular glutamate content as evaluation indexes, the fermentation medium of the strain F-5 was optimized by response surface methodology, and quadratic regression model of molasses, industrial peptone and KH2PO4 was established. The optimum formula of fermentation medium were determined as follows: molasses (30% sucrose) 100 ml/L, yeast extract powder 10 g/L, industrial peptone 20 g/L, MgSO4·7H2O 1 g/L, K2HPO4 0.5 g/L, and FeSO4·7H2O 2 g/L. The content of intracellular free glutamate was up to 3.29% under the optimum medium at 24 h, which was 87.8% higher than that of before optimization.

Key words

Saccharomyces cerevisiae / intracellular free glutamate / fermentation medium / response surface methodology

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LIU Licong1, HU Yiwen1, WANG Zhi1, DAI Jun1, LI Xin1, YAO Juan2, ZHENG Guobin2, LV Zhengbo3, CHEN Xiong1*. Screening of Saccharomyces cerevisiae with high glutamic acid content and optimization of fermentation medium[J]. China Brewing, 2017, 36(6): 116-120 https://doi.org/10.11882/j.issn.0254-5071.2017.06.024

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