Optimization of immobilized conditions of yeast protease by porous chitosan and analysis of its enzymatic properties
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1. College of Food Science, Shihezi University, Shihezi 832000, China;
2.College of Information Science and Technology, Shihezi University, Shihezi 832000, China;
3.Xinjiang Tang-Ting-Xia-Lu Chateau Co., Ltd., Wujiaqu 831300, China
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Published
2017-01-25
Issue Date
2017-02-08
Abstract
The yeast protease was immobilized by porous chitosan microspheres. The glutaraldehyde content, adsorption time, immobilization temperature and pH were determined by single factor and orthogonal experiments. Using the protease activity recovery as evaluation index, the immobilized conditions were as follows: glutaraldehyde content 1.4%, adsorption temperature 27 ℃, pH 10 and adsorption time 24 h. Under the optimal conditions, the recovery rate of immobilized yeast protease activity was 68.8%. The results of enzymatic properties analysis showed that the optimum reaction temperature of immobilized enzyme was 10 ℃ higher than that of free enzyme, and its optimal pH moved to alkaline direction by 1 pH unit compared with the free enzyme. Therefore, embedding of yeast protease by porous chitosan could improve the protease activity.
YAO Xiaoruining1, GAO Feifei1, WANG Bin1, XIAO Jing2, JIA Chenkun1, WANG Shunli3, SHI Xuewei1*.
Optimization of immobilized conditions of yeast protease by porous chitosan and analysis of its enzymatic properties[J]. China Brewing, 2017, 36(1): 146-150 https://doi.org/10.11882/j.issn.0254-5071.2017.01.031
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