Histamine commonly exists in various foods. In the rapid detection process of histamine using histamine molecularly imprinted polymers sensor, the ratio of template molecule and functional monomers has distinct influence on the specificity adsorption capacity. Histamine and 2-(trifluoromethyl) acrylic acid were selected as template molecule and functional monomer respectively. For exploring the most appropriate ratio, the ultraviolet spectrum was used to detect the maximum absorption peaks of histamine and 2-(trifluoromethyl) acrylic acid respectively. Furthermore, differential ultraviolet spectra and ultraviolet spectra were used to various functional monomer with different ratios. Results showed that the pre-assembly system got stable at the ratio of 1∶4, and the most stable situation was aquired at the ratio of 1∶6 with the decrease of the maximum absorption peak and the red shift of wavelength. The results showed a definite guidance for manufacturing histamine molecularly imprinted polymers sensor to the histamine rapid detection.
Key words
histamine /
molecularly imprinted polymers /
template-monomers ratio /
ultraviolet spectrum
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