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Preparation of gallic acid from tannic acid by Aspergillus niger B1401
JIAN Hui1,2, WU Xinying1,2*, QIU Shuyi1,2, REN Jiaming1,2
China Brewing ›› 2016, Vol. 35 ›› Issue (4) : 24-28.
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Preparation of gallic acid from tannic acid by Aspergillus niger B1401
The optimal technological conditions of gallic acid preparation from tannic acid was studied in the submerged fermentation by Aspergillus niger B1401. Results showed that the fed-batch adding tannin method was better. The optimum process conditions were obtained as follows: total adding tannins concentration 204 mg/ml, pH 4.0, reactive temperature 30 ℃, and rotate speed 160 r/min. At the same conditions, the experiments were carried out in 5 L automatic fermentor, the conversion efficiency was better than that in shake flask fermentation. The transformation balance time was shortened to 44 h from 48 h, the yield of gallic acid increased from 70.35% to 74.04%, and the gallic acid concentration increased from 144 mg/ml to 161 mg/ml.
Aspergillus niger B1401 / tannase / biotransformation / gallic acid {{custom_keyword}} /
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