利用4-硝基苯基-β-D吡喃葡萄糖苷为底物测定酵母中的β-葡萄糖苷酶,研究8株酿酒酵母在上清液、壁膜间隙和细胞内的β-葡萄糖苷酶活性及氧气对酿酒酵母产β-葡萄糖苷酶的影响.结果表明β-葡萄糖苷主要位于细胞间隙和细胞内,酿酒酵母M4产β-葡萄糖苷酶最高,为4.1μmolpNP·mL-1·h-1.氧气显著促进酿酒酵母合成β-葡萄糖苷酶,且相对于厌氧条件,有氧条件下酿酒酵母M4的β-葡萄糖苷酶增加了4.51倍.

To study the enzyme location and the effect of oxygen on β-glucosidase activity, eight Saccharomyces cerevisiae stains were evaluated using 4-nitrophenyl-β-D glucopiranoside(pNPG) as substrate in supernatant, periplasmic space and intracellular under aerobic and anaerobic conditions. The results showed that most enzymes were located in both periplasmic space and intracellular. S. cerevisiae M4 had the highest β-glucosidase activitiy[4.1μmol pNPG·(ml·h)-1]. Aerobic growth condition significantly stimulated β-glycosidase biosynthesis in S. cerevisiae stains. S. cerevisiae M4 showed 4.51-fold more enzymatic activity under aerobic condition than under anaerobic condition.