有机溶剂萃取分离水中L-色氨酸和L-谷氨酸

张剑;杨新妮;杨平;高冰

中国酿造 ›› 2013, Vol. 32 ›› Issue (5) : 126-128.

PDF(214 KB)
PDF(214 KB)
中国酿造 ›› 2013, Vol. 32 ›› Issue (5) : 126-128.
研究报告

有机溶剂萃取分离水中L-色氨酸和L-谷氨酸

  • 张剑;杨新妮;杨平;高冰
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Separation and purification of L-tryptophan and L-glutamic acid in aqueous solution with organic solvent extraction

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摘要

以正辛醇为稀释剂、二(2-乙基己基)磷酸为萃取剂,研究了L-色氨酸和L-谷氨酸在有机相中的萃取分配行为.在pH=2.0时,L-色氨酸从水相转入有机相,实现L-谷氨酸与L-色氨酸的分离.使用等体积的1mol/L HCl溶液将负载有机相中的L-色氨酸反萃回水相,L-色氨酸的平均回收率为70.8%.2种氨基酸混合水溶液经有机溶剂2次萃取后,可得L-谷氨酸纯品,其平均回收率为97.6%.

Abstract

Extraction distribution behaviors of L-tryptophan and L-glutamic acid in organic phase were investigated by using n-caprylic alcohol and di(2-Ethylhexyl) phosphoric acid (D2EHPA) as deluent and extractant, respectively. L-tryptophan could be separated from the mixture of two amino acids in that it was extracted into D2EHPA phase from aqueous solution of a acidity at pH 2.0. The same volume of 1 mol/L HCl solution was adopted as stripping agent of L-tryptophan in the loaded organic phase. Average recovery of L-tryptophan was 70.8%. After the mixture of 2 amino acids had been extracted twice, single L-glutamic acid product could be available and its average recovery was 97.6%.

关键词

L-色氨酸 / L-谷氨酸 / 二(2-乙基己基)磷酸 / 溶剂萃取 / 分离纯化

Key words

L-tryptophan / L-glutamic acid / di(2-Ethylhexyl)Phosphoric acid / solvent extraction / separation and purification

引用本文

导出引用
张剑;杨新妮;杨平;高冰. 有机溶剂萃取分离水中L-色氨酸和L-谷氨酸[J]. 中国酿造, 2013, 32(5): 126-128
ZHANG Jian, YANG Xinni, YANG Ping, GAO Bing. Separation and purification of L-tryptophan and L-glutamic acid in aqueous solution with organic solvent extraction[J]. China Brewing, 2013, 32(5): 126-128
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