To detect Salmonella enteritidis rapidly, loop-mediated isothermal amplification (LAMP) method was developed. According to Salmonella enteritidis serotype specific gene lygD, LAMP inner and outer primer sets were designed. Specificity of LAMP primers were validated by assaying 10 different strains. Sensitivity of LAMP method was evaluated by serial gradient dilution of Salmonella enteritidis solution. LAMP used for the detection of egg samples. The detection limits of LAMP assay were 2.33×101cfu/ml for pure cultures and 2.67×101cfu/ml for analogy egg samples. LAMP method is a simple, rapid and feasible method for the detection of Salmonella enteritidis .