研究了利用RP-HPLC对干酪中黄曲霉毒素M1进行测定,分别采用乙腈、甲醇、丙酮作为提取溶剂对干酪样品中的黄曲霉毒素M1进行提取,利用一种亲水亲脂平衡柱萃取黄曲霉毒素M1,然后上机检测,采用质量分数为25％的乙腈水溶液为流动相,流速1 mL/min,利用荧光检测器进行检测.结果表明,采用乙腈作为干酪中黄曲霉毒素M1的提取试剂比其他2种溶剂更为适宜;OASIS HLB固相萃取净化柱对于黄曲霉毒素M1的分离纯化效果很好；干酪中的黄曲霉毒素在8mm内被快速检出,平均同收率较高,相对标准偏差(RSD)为1.4％～5.7％,最低检出限为0.037μg/kg.该方法是一种针对干酪中黄曲霉毒素M1的快速高效的检测方法.

 Using RP-HPLC technique to detect aflatoxin M1 in cheese. Adhibition acetonitrile, methanol and acetone extract respectively aflatoxin M1 in cheese. Then, aflatoxin M1 were extracted by Hydrophile-Lipophile Balance column to again, and analyzed by HPLC. The mobile phase was acetonitrile-water (25%). The flow-rate was 1mL/min. Fluorescence detector was applied. The experimental result indicated that the using of acetonitrile for extracting the aflatoxin M1 in cheese was a more suitable extraction way than others. The procedure of SPE was benefit of improving the purity of aflatoxin M1 . The aflatoxin M1 in cheese was rapidly detected in 8min, and the recovery rate of aflatoxin M1 was relatively high. The relative standard deviation was 1.4%~5.7%. The limit of detection was 0.037μg/kg. So this experimental method is a rapid, efficient detection way for the aflatoxin M1 in cheese.