分别用水、甲醇、乙醇、乙酸乙酯、石油醚对益智进行提取,采用滤纸片法比较不同溶剂提取物对大肠杆菌、金黄色葡萄球菌及黑曲霉的抑制作用；通过二倍稀释法确定益智的80％vol乙醇提取物对大肠杆菌、金黄色葡萄球菌及黑曲霉菌的最小抑菌浓度分别为3.00mg/mL、1.50mg/mL和24.00mg/mL；并确定热处理、紫外线、介质pH值对益智的80％vol乙醇提取物抑菌活性无显著影响.

The antimicrobial activity of the fructus Alpinia oxyphylla (AOF) was determined by filter paper method with different extraction solvent
such as water, methanol, ethanol, ethyl acetate, petroleum ether. With the minimal inhibiting concentration (MIC) detected by the broth two-fold agar
dilution method for Escherichia coli, Staphylococcus aureus and Aspergillus niger being 3.00mg/ml, 1.50mg/ml and 24.00mg/ml respectively, the re-
sults showed that the 80%vol ethanol-extract from the AOF represented significant antimicrobial activity. And good stabilities under different p H val-
ue, temperature and UV irradiation time were obtained.