The process of Flammulina velutipes yogurt were optimized by orthogonal test. The results showed that after mingling the juice of Flammulina velutipes and milk, coud significantly increase the total number of Lactobacillus, adding 10% extract of Flammulina velutipes, sugar 7%, yogurt inoculation 4%, under 41℃, the better quality Flammulina velutipes yogurt could be obtained after fermenting 4h.