从泡菜中筛选到一株高产共轭亚油酸(Conjugated Linoleic Acid,CLA)的菌株.经生理生化鉴定及16S rDNA序列分析,鉴定为嗜酸乳杆菌,命名为L44.为了提高南瓜籽油中共轭亚油酸的产量,对嗜酸乳杆菌的培养条件进行优化,采用5因素进行单因素试验设计,然后通过二次旋转组合设计进行优化.确定最优组合为培养时间24h,培养温度37℃,pH值7,接种量10％,装液量150mL,同时所得CLA产量从95.1425μg/mL提高为107.0989μg/mL.

A strain, L44, which could improve the output of conjugate lineleic acid (CLA）, was isolated from kraut. The strain was identified belongs to Lactobacillus acidophilus by a series of morphological and biochemical characteristics analysis and 16S rDNA, which was named as L44. Cultural conditions in a bid for higher production of conjugate lineleic acid of L44 were optimized by five factors single-factor design and quadratic regression orthogonal rotary test. The determined conditions were incubation for 24h at 37℃ with pH value 7, and inoculum size 10% and the filling rate of liquid was 150ml. The production of CLA increased up to 107.0989μg/ml from 95.1425μg/ml.