The nitrogenous components in dry rice wine with polypeptides were studied by chromatographic analysis to provide a theoretical basis for the functional properties. The results showed that the nitrogenous components of the products had the following characteristics: (1) the average contents of acid-soluble proteins, free amino acids, and polypeptides in dry rice wine reached 1002.5mg/100ml, 191.39mg/100ml (the proportion of 7 kinds of essential amino acids was 36.65% of the total amino acids) and 811.11mg/100ml, respectively. (2) The polypeptides in dry rice wine were small molecular peptides, and peptides with molecular weight lower than 500u accounted for 81.1%. The molecular weight patterns of dry rice wine were similar to that of the corn polypeptide standard patterns. Therefore, the products have a certain effect of nutrition and health.