为了获得赤藓糖醇高产菌株,以酿酒酵母NJWGYH30566为研究对象,利用N+注入进行诱变.注入N+的能量为15keV,剂量为20×1014cm-2、40× 1014cm-2、60× 1014cm-2、80×1014cm-2、100×1014cm-2、120×1014cm-2.结果表明最佳注入剂量为80×1014cm-2,选育出一株赤藓糖醇高产菌株H-32,赤藓糖醇产量提高17.52％,且副产物甘油降低26.9％,经连续10代遗传稳定性试验考察,其高产性能稳定,极大提高了生产效率.

N+ ion implantation was used to mutate Saccharomyces cerevisiae NJWGYH30566 for obtaining higher-yield erythritol strain. The original strain was mutated by 15keV N+ ion implantation with the dose of 20×1014cm-2, 40×1014cm-2, 60×1014cm-2, 80×1014cm-2, 100×1014cm-2, and 120×1014cm-2, respectively. Results showed that the optimal N+ ion dose was 80×1014cm-2, and a strain of high-yield erythritol was obtained and named as H-32. Erythritol yield of the mutant was increased by 17.52%, and by-product glycerol was decreased by 26.9% compared to those of the original strain. Subculture results showed that the mutant strains had high genetic stability.