A strain with high inulinase productivity was screened, and identified as Eladia saccula SA1201 through observation by morphologic and identification of 18S rRNA gene partical sequence. The fermentation conditions of the strain were optimized by orthogonal test. the optimal culture medium consisted of 30.0g/L inulin, 7.0g/L peptone, 5.0g/L NaCl, 3.0g/L K2HPO4·3H2O, initial pH value 6.0, medium volum 50ml/250ml, 30℃ and 160r/min for 3d.