To establish a method for determination of gastrodin,p-hydroxybenzyl alcohol and p-hydroxybenzaldehyde in ethanol extracts fromGastrodia tuber and culture medium of G.frondosa.HPLC was used in the study.The compounds were eluted using a Agilent TC-C18(4.6mm×250mm,5μm) column,and detected at 221 nm with a flow rate of 1ml/min.The column tem-perature was set at 30℃.Gradient solvent A was 0.1% phosphoric acid;solvent B was acetonitrile,starting from 3% B to 30% B for 0~35min,30%~70% B for 35min~45min.Under the above condition,good linearity was obtained for three substances with a correlation coefficient 0.9999,recoveries ranged between 96% and 102%,while the precision of the method was <5%(RSD).The content of three substances in culture medium of Grifola frondosa can be accurately determine simultaneously by this method.Lay the foundation for the further exploration of interaction ofG.frondosa metabolism and the ethanol extracts fromG.tuber.
XU Xiaobao;WU Tianxiang;ZHANG Yong;TAN Sha;WANG Na;FU Hongwei.
Determination of extracts from Gastrodia tuber fermented with Grifola frondosa by HPLC method[J]. China Brewing, 2012, 31(5): 182-185