白果富含银杏内酯和黄酮等多种活性成分以及银杏酚酸和氢氰酸等有毒物质,利用生物工程技术开发利用白果,降低白果毒性提高其功效关键是首先要将白果水解.该文通过单因素试验和控制变量法,确定了用双酶法水解白果工艺条件.所采用的酶为液化酶和糖化酶,其最佳的液化工艺条件为:液化温度为80℃,液化时间为20min,液化酶添加量为0.5％；最佳糖化工艺条件为:糖化温度为60℃,糖化时间为20min,糖化酶的添加量为0.25％,该课题的研究为进一步通过生物工程技术开发利用白果奠定了基础.

Ginkgo contains various of active ingredients such as ginkgolide and flaronoid,as well as some harmful ones,including ginkgolic acid and hydrogen cyanism.To make use of ginkgo by biotechnic,the key step of lowing toxicity and enhanceing its active is hydrolyzis.By single factor experiment and controlling variables method,the optimum conditions of liquefying and mashing in ginkgo liquor by two-enzyme were determined.When ginkgo was liquefied 20min by 0.5% liquefying enzyme in 80℃ and mashed 20min by 0.25% glucase in 60℃,the content of producing sugar would be the highest.This research laid a foundation for the further biotechnical development of ginkgo.