生物制麦中白地霉的筛选及其特性的研究

彭涛;刘琦;王千存;张怀予;路宏科;马文锦;陈兴叶;殷欣

中国酿造 ›› 2012, Vol. 31 ›› Issue (11) : 73-77.

PDF(1215 KB)
PDF(1215 KB)
中国酿造 ›› 2012, Vol. 31 ›› Issue (11) : 73-77.
研究报告

生物制麦中白地霉的筛选及其特性的研究

  • 彭涛;刘琦;王千存;张怀予;路宏科;马文锦;陈兴叶;殷欣
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Isolation and characteristics of Geotrichum candidum in the micromalting

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摘要

以甘啤3号大麦为原料,从中分离得到了2株生长能力较强的菌株,通过对该菌产生的β-葡聚糖酶、木聚糖酶、纤维素酶和糖化酶的酶活情况进行研究及生理生化鉴定得出该菌株为白地霉,分别为白地霉GQG28-1和白地霉GQG28-2.对筛选到的白地霉GQG28生长特性的初步研究表明,该菌最适生长温度为25℃,生长温度范围为5℃~37℃,其最适生长pH 5.6,最低生长pH值为3.2,最高生长pH值为11.0,利用该白地霉制得麦芽综合指标为206.15,所制麦芽糖化力为308.5WK、α-氨基氮为186mg/100g、浸出物相对质量分数为87.1%.

Abstract

Two strains of fungus were isolated from Gansu NO.3 beer barley. The two strains were primarily identified as Geotrichum candidum Link GQG28-1 and Geotrichum candidum Link GQG28-2, respectively, by identification of physiological and biochemical properties and the activities of β-glucanase, xylanase, protease and glucamylase produced. The results of growth characteristics of G. candidum Link GQG28 showed that the optimum growing temperature was 25℃ in the range of 5℃~37℃. The optimum pH was 5.6 and it could grow at pH 3.2-11.0. The practical malt quality was 206.15, the saccharification rate of malt was 308.5WK, the α-amino state nitrogen was 186mg/100g, and the extraction rate was 87.1% when G. candidum Link is used .

关键词

生物制麦 / 大麦 / 白地霉 / 酶活 / 特性

Key words

micromalting / barley / Geotrichum candidum Link / enzyme activities / characteristics

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彭涛;刘琦;王千存;张怀予;路宏科;马文锦;陈兴叶;殷欣. 生物制麦中白地霉的筛选及其特性的研究[J]. 中国酿造, 2012, 31(11): 73-77
Isolation and characteristics of Geotrichum candidum in the micromalting[J]. China Brewing, 2012, 31(11): 73-77
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