以高产诱变株紫红曲霉M144为出发菌株,以红色价和色调为指标,对液态发酵生产红曲红色素的培养基进行响应面法优化.首先采用析因实验设计确定液态发酵生产红曲红色素的红色价和色调的主要影响因子,然后通过Box-Behnken实验设计和响应面法进行优化.实验结果表明:玉米粉和豆粕粉是红色价的主要影响因子,玉米粉和MgSO4·7H2O是色调的主要影响因子,最优培养基配方为:玉米粉4.52％,豆粕粉2.32％,MgSO4·7H2O 1.55％,K2HPO4·3H2O 0.10％.在此条件下,红曲红色素的红色价为280.27U/mL,色调为1.09,比优化前分别提高了64.86％和10.10％.

The culture medium of monascus red pigments by Monascus purpureus M144 strain in submerged fermentation was optimized by Box- Behnken experiment and response surface methodology using red value and color tone as indicators. M. purpureus M144 strain was obtained by ultraviolet mutation and had good ability of producing red pigment. The results showed corn flour and bean cake flour were the main factors affecting red value, and corn flour and MgSO4·7H2O were the main factors affecting color tone. The optimal culture medium was as followed: corn flour 4.52%, bean cake flour 2.32%, MgSO4·7H2O 1.55% and K2HPO4·3H2O 0.10%. Under these conditions, the red value and color tone of pigments reached to 280.27U/ml and 1.09, which were 64.86% and 10.10% higher than those before optimization, respectively.