氨基酸液生产过程中污染菌的鉴定

刘思喜;刘天明;王红

中国酿造 ›› 2012, Vol. 31 ›› Issue (10) : 128-130.

PDF(672 KB)
PDF(672 KB)
中国酿造 ›› 2012, Vol. 31 ›› Issue (10) : 128-130.
研究报告

氨基酸液生产过程中污染菌的鉴定

  • 刘思喜;刘天明;王红
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Identification of contaminated microorganisms during the amino acid solution production

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摘要

从氨基酸液中分离出8株菌,命名为ZH-1~ZH-8.7株属于细菌,1株属于霉菌.利用16S rDNA区序列分析并结合形态学、生理学特征对细菌菌株进行鉴定.其中,ZH-4为Psychrobacter,ZH-5为Lysinibacilus,ZH-6为Aerococcus vinidians,ZH-7为Bacillus pumilus;ZH-3与Planococcus citreus,ZH-2与Kocuria palustris,ZH-1与Bacillus pumilus可能为近缘种,有待进一步鉴定.生理生化鉴定结果与模式菌株相吻合的菌株为ZH-2~ZH-7.18S rDNA区序列分析结合形态学特征鉴定ZH-8为Penicillium.ZH-1~ZH-8在GenBank的登录号依次为JQ819720~JQ819726,JX192597.

Abstract

Eight strains of microorganisms were isolated from the amino acid solution, which were labeled as ZH-1~ZH-8, from which seven were bacterium and one was fungal. Strains were identified based on the 16S rDNA sequence analysis, combined with morphological and physiological characterization analysis. The results showed that ZH-4 belongs to Psychrobacter sp., ZH-5 Lysinibacilus, ZH-6 Aerococcus viridians, ZH-7 Bacillus pumilus. Strains ZH-3 and Planococcus citreus, strain ZH-2 and Kocuria palustris, and strain ZH-1 and Bacillus pumilus may be colosed species, which need further identification. The physiological results indicated that strains of ZH-2 - ZH-7 were well coincident with model strain. Based on the 18S rDNA sequence analysis and morphological characterization,ZH-8 is identified as Penicillium. The accession number of the strains ZH-1~ZH-8 on GenBank is JQ819720~JQ819726 and JX192597.

关键词

氨基酸液 / 污染菌 / 16S rDNA / 18S rDNA / 鉴定

Key words

amino acid solution / contaminated microorganism / 16S rDNA / 18S rDNA / identification

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刘思喜;刘天明;王红. 氨基酸液生产过程中污染菌的鉴定[J]. 中国酿造, 2012, 31(10): 128-130
Identification of contaminated microorganisms during the amino acid solution production[J]. China Brewing, 2012, 31(10): 128-130
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