Eight strains of microorganisms were isolated from the amino acid solution, which were labeled as ZH-1~ZH-8, from which seven were bacterium and one was fungal. Strains were identified based on the 16S rDNA sequence analysis, combined with morphological and physiological characterization analysis. The results showed that ZH-4 belongs to Psychrobacter sp., ZH-5 Lysinibacilus, ZH-6 Aerococcus viridians, ZH-7 Bacillus pumilus. Strains ZH-3 and Planococcus citreus, strain ZH-2 and Kocuria palustris, and strain ZH-1 and Bacillus pumilus may be colosed species, which need further identification. The physiological results indicated that strains of ZH-2 - ZH-7 were well coincident with model strain. Based on the 18S rDNA sequence analysis and morphological characterization,ZH-8 is identified as Penicillium. The accession number of the strains ZH-1~ZH-8 on GenBank is JQ819720~JQ819726 and JX192597.