Some metal ions have great effects on production of the halogen alcohol dehalogenase by fermentation with recombinant E.coli.Firstly,optimal concentrations of some typical trace elements were obtained through the single factor experiments.Secondly,significant factors and their inflection points were determined by the two-level factorial experiments and steepest ascent experiments.The significant factors are ZnSO4·7H2O,CoCl2·6H2O and FeSO4·7H2O.Finally,Response surface methodology was utilized to determine the optimal ratio of the trace elements,which were FeSO4·7H2O 11.86g/L,ZnSO4·7H2O 2g/L,MnSO4·H2O 1g/L,CaCl2 0.05g/L,CoCl2·6H2O 1.2g/L,CuSO4·5H2O 0.8g/L,respectively.Compared with the control,the enzyme activity increased by 2-fold when the optimal ratio metal ions were added to the medium.
LI Jianhua;LI Xiao;ZHANG Ya;XU Neng;YAO Juan;LI Zhihong;YU Xuefeng.
Effects of metal ions on production of the halogen alcohol dehalogenase[J]. China Brewing, 2012, 31(3): 127-131 https://doi.org/10.3969/j.issn.0254-5071.2012.03.035