该研究以具有耐盐碱、防病、促生的复合微生物菌剂为研究对象,采用单因素试验和正交试验对复合微生物菌剂种子、发酵培养基成分及发酵工艺进行优化,以复合菌剂生物量（OD600 nm值）、固氮量、解钾量、溶有机磷量、溶无机磷量和抑菌率为考察指标,采用Topsis综合评价法确定最佳培养条件。结果表明,复合微生物菌剂最适种子培养基为2%酵母浸出膏、1%乳糖、1% MgSO4;最适发酵培养基为0.1%豆饼粉、1.5%玉米浆、1.5% CaCl2;最佳发酵工艺为pH 6、接种量9%、转速180 r/min、发酵温度35 ℃、发酵时间35 h。此优化发酵条件下,复合菌剂的OD600 nm值为1.38、固氮量为0.23 g/L、解钾量为101.00 mg/L、溶有机磷量为504.54 μg/mL、溶无机磷量为658.82 μg/mL、抑菌率为60.97%。
Abstract
Using the compound microbial agent with saline-alkali resistance, disease prevention and growth promotion as the research object, the composite microbial agent, fermentation medium composition and fermentation process were optimized by single factor tests and orthogonal tests. The biomass of compound microbial agent, the amount of nitrogen fixed, potassium solubilized, organic phosphorus solubilized, inorganic phosphorus solubilized and bacteria inhibitive rate were used as the investigation indexes. Topsis comprehensive evaluation method was used to determine the optimal culture conditions. The results showed that the optimal seed medium for the compound microbial agent was yeast extract 2%, lactose 1%, MgSO4 1%. The optimal fermentation medium was soybean cake powder 0.1%, corn pulp 1.5% and CaCl2 1.5%. The optimum fermentation process were as follows: pH 6, inoculum 9%, rotation speed 180 r/min, fermentation temperature 35 ℃ and time 35 h. Under these optimal conditions, the OD600 nm value of compound bacteria was 1.38, the amount of nitrogen fixed, potassium solubilized, organic phosphorus solubilized and inorganic phosphorus solubilized was 0.23 g/L, 101.00 mg/L, 504.54 μg/ml and 658.82 μg/ml, respectively, and bacteria inhibitive rate was 60.97%.
关键词
耐盐碱微生物 /
生防菌 /
复合微生物菌剂 /
发酵工艺 /
培养基 /
优化
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Key words
saline-tolerant microorganisms /
microbial antagonist /
compound microbial agent /
fermentation process /
medium /
optimization
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脚注
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基金
甘肃省农业科学院重点研发计划（2023GAAS22）; 甘肃省现代农业科技支撑体系区域创新中心重点科技项目（2022GAAS02）
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