Preparation of Andrias davidianus liver peptide wine and its protective effect on mouse liver after drinking
ZENG Yunhao, SU Wen, ZHENG Xiaoli, WANG Jinghua, CHEN Dejing
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(1.College of Biological Sciences and Engineering, Shaanxi University of Technology, Hanzhong 723001, China; 2.Key Laboratory of Resource Biology, Shaanxi University of Technology, Hanzhong 723001, China; 3.Hanzhong City Science and Technology Resource Coordination Center, Hanzhong 723001, China)
The giant salamander liver peptide (GSLP) wine was prepared with Andrias davidianus liver and Baijiu as raw materials. Liver peptides with different molecular mass were prepared by enzymatic hydrolysis, and the in vitro alcohol dehydrogenase (ADH) activation activity, in vitro antioxidant activity and solubility were measured. Mice were administrated with normal saline, Baijiu and A. davidianus liver peptide wine, respectively, and the related biochemical indexes in the liver and serum of mice were determined. The results showed that the liver peptide with molecular weight<3 000 Da (GSLP-Ⅲ) had the highest ADH activation rate (83.53%), the scavenging rates of 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH·), hydroxyl radical (·OH) and superoxide anion free radical (O2-·) were all>83.50%, the reducing power was the highest (the absorbance value was 0.44), and the solubility in Baijiu (53%vol) was high (94.35%). Compared with the mice in Baijiu group, the ADH, acetaldehyde dehydrogenase (ALDH), superoxide dismutase (SOD) activity and reduced glutathione (GSH) content of mice liver treated with A. davidianus liver peptide wine group increased by 66.58%, 26.84%, 15.84% and 36.92%, respectively. But the malondialdehyde (MDA) content, triglyceride (TG) content, alanine aminotransferase (ALT) activity and aspartate transaminase (AST) activity of serum decreased by 17.37%, 26.39%, 21.82% and 23.61%, respectively, indicating A. davidianus liver peptide wine had certain protective effect on mouse liver after drinking.
ZENG Yunhao, SU Wen, ZHENG Xiaoli, WANG Jinghua, CHEN Dejing.
Preparation of Andrias davidianus liver peptide wine and its protective effect on mouse liver after drinking[J]. China Brewing, 2023, 42(2): 120-125 https://doi.org/10.11882/j.issn.0254-5071.2023.02.020