将雄性昆明（KM）小鼠随机分为溶剂对照组、模型对照组和植物乳杆菌（Lactobacillus plantarum）HCS03-001菌粉低（1 g/（kg bw·d））、中（2 g/（kg bw·d））、高（4 g/（kg bw·d））剂量组，连续灌胃14 d后建立功能性便秘模型，考察植物乳杆菌HCS03-001对小鼠小肠墨汁推进率的影响。此外，将小鼠分为溶剂对照组及植物乳杆菌HCS03-001低、中、高剂量组，连续灌胃14 d，分别采集小鼠灌胃0 d和14 d的新鲜粪便进行培养和菌落计数，考察小鼠肠道内菌群的变化情况，旨在研究植物乳杆菌HCS03-001改善小鼠功能性便秘和调节肠道菌群的作用。结果表明，植物乳杆菌HCS03-001中、高剂量可显著提高小鼠小肠墨汁推进率（P＜0.05）；植物乳杆菌HCS03-001高剂量可降低产气荚膜梭菌（P＜0.05）、肠球菌（P＜0.05）、肠杆菌（P＞0.05）的活菌数，同时促进乳杆菌数的增殖（P＜0.05）。说明植物乳杆菌HCS03-001具有改善小鼠便秘、调节肠道菌群的功能。

Male Kunming (KM) mice were randomly divided into solvent control group, model control group, Lactobacillus plantarum HCS03-001 low- dose (1 g/(kg bw·d)), medium-dose (2 g/(kg bw·d)) and high-dose (4 g/(kg bw·d)) groups, the functional constipation model was established after continuous gavage for 14 d, and the effect of L. plantarum HCS03-001 on intestinal ink progradation rate of mice was investigated. In addition, the mice were divided into solvent control group and L. plantarum HCS03-001 low-dose, medium-dose and high-dose groups for continuous gavage for 14 d. The fresh feces of the mice on 0 d and 14 d were collected for culture and colony count, and the changes of intestinal flora of the mice were investigated, to study the effect of L. plantarum HCS03-001 on improving functional constipation and regulating intestinal flora in mice. The results showed that L. plantarum HCS03-001 at medium- and high-dose could significantly increase the intestinal ink progradation rate of mice (P＜0.05), and L. plantarum HCS03-001 at high-dose could significantly reduce the viable count of Clostridium perfringens (P＜0.05), Enterococcus (P＜0.05) and Enterobacter (P＞0.05), and promote the proliferation of Lactobacillus number (P＜0.05), indicating that L. plantarum HCS03-001 could improve constipation and regulate intestinal flora in mice.