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甲基营养型芽孢杆菌产3-羟基丁酮的微孔板发酵培养基优化
Optimization of microplate fermentation medium for acetoin-producing Bacillus methylotrophicus
为获得一种高效、快速的筛选高产3-羟基丁酮菌株的方法，首先对诱变后的34株突变株分别进行摇瓶发酵和48孔板发酵，验证两种方法之间的相关系数R为0.806 2，表明两种方法具有较好的一致性，48孔板可以用于产3-羟基丁酮菌株的大批量筛选。然后采用响应面分析法对48孔板发酵培养基组分进行优化。结果表明，在优化的条件下，3-羟基丁酮产量达到41.17 g/L，比优化前提高了30.08%，且3-羟基丁酮转化率由原来的27.31%提高到33.12%。
In order to obtain an efficient, fast method for screening strains with acetoin high-yield, 34 mutant strains obtained by mutagenesis were cultivated in shake flask and 48-well microplate fermentation, respectively. The correlation coefficient of the two fermentation methods was 0.806 2, which showed that they had a good consistency, and the 48-well microplate could be used for mass screening acetoin-producing strains. The fermentation medium components of 48-well microplate were optimized by response surface methodology. The results showed that under the optimum conditions, the acetoin yield was up to 41.17 g/L, which was 30.08% higher than that before optimization, and the conversion of acetoin increased from 27.31% to 33.12%.
甲基营养型芽孢杆菌 / 3-羟基丁酮 / 48孔板 / 响应面优化 {{custom_keyword}} /
Bacillus methylotrophicus / 3-hydroxybutanone / 48-well microplate / response surface methodology {{custom_keyword}} /
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