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嗜热脱氮芽孢杆菌产α-半乳糖苷酶影响因素的研究
Influencing factors of Geobacillus thermodenitrificans on α-galactosidase-production
该文对前期筛选出的嗜热脱氮芽孢杆菌YWX5产α-半乳糖苷酶的影响因素进行了初步的研究，通过测定α-半乳糖苷酶酶活，探究了培养基成分（包括碳源、氮源、无机盐）及培养条件（初始pH值、培养温度、培养时间）对该嗜热脱氮芽孢杆菌产α-半乳糖苷酶能力的影响。实验结果表明，对该菌产酶最有效的碳源为3%豆粕，氮源为0.5%硝酸钾，附加氮源为0.5%酵母浸出物；添加0.5%氯化钠和0.1%磷酸氢二钾有助于该菌产酶。另外，该菌最佳产酶培养温度为60 ℃，培养基最适初始pH在7.0～8.0，培养时间为65 h。
The influencing factors of the preliminarily screened Geobacillus thermodenitrificans YWX5 on α-galactosidase production were studied. By the α-galactosidase activity determination, the effect of medium composition (including carbon sources, nitrogen sources and initial pH), culture temperature and time on α-galactosidase production of G. thermodenitrificans YWX5 was investigated. The results showed the most effective carbon source was soybean meal 3%, the most effective nitrogen source was KNO3 0.5%, and enzyme production was promoted by supplementation with yeast extracts 0.5% as extra nitrogen source; it was also enhanced by supplementation with NaCl 0.5% and K2HPO4 0.1%. In addition, the optimal culture temperature, time and initial pH of the medium for the α-galactosidase-producing strain were 60 ℃, 65 h and 7.0-8.0, respectively.
嗜热脱氮芽孢杆菌；&alpha / -半乳糖苷酶；酶活；影响因素 {{custom_keyword}} /
Geobacillus thermodenitrificans / α-galactosidase / enzyme activity / influencing factors {{custom_keyword}} /
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广西科学基金（2014GXNSFAA118086）；广西科学基金（2015GXNSFBA139068）
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