
重组运动发酵单胞菌HYMX发酵葡萄糖产乙醇工艺的优化
Optimization of glucose fermentation technology for ethanol production by recombinant Zymomonas mobilis HYMX
用Tn5转座技术将七个基因(Pfu-sHSP,yfdZ,metB,xylA,xylB,tktA and talB)导入到原型菌CP4中,得到重组菌HYMX;并用响应面方法优化重组菌的发酵工艺。结果表明,最佳发酵工艺条件为葡萄糖质量浓度285 g/L,初始pH值为6.5,发酵时间63 h,发酵温度32 ℃,接种量10%,酵母提取物10 g/L。优化后最高乙醇产量为137.11 g/L,比优化前的最高乙醇产量130.9 g/L提高了4.53%,比原型菌的72.17 g/L提高了89.98%。
关键词:中图分类号:Q815 文献标识码:A 文章编号:0254-5071(2015)05-0118-05
doi:
Seven genes of Pfu-sHSP, yfdZ, metB, xylA, xylB, tktA and talB were introduced into prototype of Zymomonas mobilis CP4 via Tn5 transposition, and the fermentation technology of recombinant bacteria was optimized by response surface method. The optimum conditions for maximum ethanol production were as follows: glucose 285 g/L, initial pH 6.5, fermentation time 63 h, temperature 32 ℃, inoculum 10%, yeast extract 10 g/L. Under the optimized conditions, the ethanol yield of the strain was 137.11 g/L, 4.53% higher than the non-uptimized one (130.9 g/L), 89.98% higher than the prototype CP4 (72.17 g/L).
重组运动发酵单胞菌HYMX / 发酵 / 乙醇含量 / 响应面 {{custom_keyword}} /
recombinant Zymomonas mobilis HYMX / fermentation / ethanol content / response surface method {{custom_keyword}} /
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