
马铃薯淀粉废水高活性絮凝菌的分离鉴定
Isolation and identification of high-activity flocculation bacteria in potato starch wastewater
从马铃薯淀粉废水和黄河污水排放口附近的污泥中分离得到300株菌,对所分离的菌株进行分离纯化,初筛和复筛,得到6株絮凝活性在60%以上的细菌,对6株细菌进行形态特征分析,将复筛的6株细菌悬液按照等体积的比例进行复配,絮凝率最高的混合菌为N1和N2,达到96.41%,选取絮凝率80%以上的4组构建菌中的5株细菌,对其进行16S rDNA序列分析,鉴定结果为N1短杆菌属(Brevibacterium sp.)、N2短小芽孢杆菌(Bacillus pumilus)、N3希瓦氏菌属(Shewanella sp.)、N4赖氨酸芽孢杆茵(Lysinibacillus fusiformis)、N5解鸟氨酸拉乌尔菌(Raoultella ornithinolytica)。
Three hundred strains were isolated from potato starch wastewater and sludge in the discharge of nearby Yellow River sewage. The isolated strains were separated and purified, preliminary screened and secondary screened. 6 strains were attained, and their flocculating activity was more than 60%. On the morphological character analysis of the 6 strains, compound suspension of rescreening 6 strains was mixed according to the same volume ratio. The flocculation rate of mixed strain N1 and N2 was the highest, reaching 96.41%. 5 strains of flocculation rate of more than 80% from the 4 groups were selected to conduct 16S rDNA sequence analysis. The identification results showed that strain N1, N2, N3, N4 and N5 were Brevibacterium sp., Bacillus pumilus, Shewanella sp., Lysinibacillus fusiformis and Raoultella ornithinolytica, respectively.
马铃薯淀粉废水 / 絮凝菌 / 分离 / 鉴定 {{custom_keyword}} /
potato starch water / flocculation / isolation / identification {{custom_keyword}} /
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甘肃省应用技术研究与开发计划(1004TCYA018)
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