利用16S rDNA序列分析法,对分离自土壤产蛋白酶和淀粉酶及拮抗物质的芽孢杆菌B15进行菌种鉴定.通过序列比对及构建系统发育树,表明菌株B15与Bacillus amyloliquefaciens subsp.plantarum AS43.3相似性达99％,鉴定为解淀粉芽孢杆菌植物亚种.菌株B15经过2次紫外线诱变,蛋白酶产量提高到初始菌株的147.1％.试验发现诱变株的淀粉酶产量与蛋白酶产量呈正相关.

Bacillus sp.B15 which produces protease,amylase and antagonistic substance was identified by 16S rDNA sequence analysis.The homology of 16S rDNA sequence of the strain B15 was 99％ compared with Bacillus amyloliquefaciens subsp.plantarum AS43.3,and the strain B15 was identified as Bacillus amyloliquefaciens subsp.plantarum.The protease activity of the mutant strain which through the twice UV mutation was 147.1％ of the original strain B15.The amylase production was positively correlated with protease production in this study.